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Literature summary for 6.4.1.6 extracted from
- Assay and properties of acetone carboxylase, a novel enzyme involved in acetone-dependent growth and CO2 fixation in Rhodobacter capsulatus and other photosynthetic and denitrifying bacteria (1997), Microbiology, 143, 755-766.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| acetyl-CoA | - |
Paracoccus pantotrophus |  |
| acetyl-CoA | dependent on, gives more reproducible and larger degree of stimulation than CoA | Rhodobacter capsulatus | |
| CoA | stimulates acetone-dependent CO2 fixation | Rhodobacter capsulatus | |
| NAD(P)H | - |
Rhodobacter capsulatus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | in high concentrations | Rhodobacter capsulatus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
acetone | - |
Rhodobacter capsulatus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required for assay, stimulating | Rhodobacter capsulatus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
x * 70000 + x * 85000, also 60000 Da and small amounts of 35000 Da subunits exist, SDS-PAGE | Rhodobacter capsulatus |
| 85000 | - |
x * 70000 + x * 85000, also 60000 Da and small amounts of 35000 Da subunits exist, SDS-PAGE | Rhodobacter capsulatus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetone + CO2 + ATP + H2O | Rhodobacter capsulatus | initial step in acetone metabolism | acetoacetate + AMP + phosphate | - |
? | |
| acetone + CO2 + ATP + H2O | Rhodobacter capsulatus PAS100 | initial step in acetone metabolism | acetoacetate + AMP + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paracoccus pantotrophus | - |
LMD82.1 | - |
| Paracoccus pantotrophus LMD82.1 | - |
LMD82.1 | - |
| Rhodobacter capsulatus | - |
PAS100 | - |
| Rhodobacter capsulatus PAS100 | - |
PAS100 | - |
| Rhodomicrobium vannielii | - |
RB4 | - |
| Rhodomicrobium vannielii RB4 | - |
RB4 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ion-exchange chromatography | Rhodobacter capsulatus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| acetone + CO2 + ATP + 2 H2O = acetoacetate + AMP + 2 phosphate | biotin-independent, activity is strongly induced by growth on acetone | Rhodobacter capsulatus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
a few hundred pmol per minute and mg protein | Rhodobacter capsulatus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C | Rhodobacter capsulatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetone + CO2 + ATP + H2O | - |
Rhodomicrobium vannielii | acetoacetate + AMP + phosphate | - |
? | |
| acetone + CO2 + ATP + H2O | - |
Paracoccus pantotrophus | acetoacetate + AMP + phosphate | - |
? | |
| acetone + CO2 + ATP + H2O | phosphate buffer, CoA and magnesium ions needed | Rhodobacter capsulatus | acetoacetate + AMP + phosphate | - |
? | |
| acetone + CO2 + ATP + H2O | initial step in acetone metabolism | Rhodobacter capsulatus | acetoacetate + AMP + phosphate | - |
? | |
| acetone + CO2 + ATP + H2O | phosphate buffer, CoA and magnesium ions needed | Rhodobacter capsulatus PAS100 | acetoacetate + AMP + phosphate | - |
? | |
| acetone + CO2 + ATP + H2O | initial step in acetone metabolism | Rhodobacter capsulatus PAS100 | acetoacetate + AMP + phosphate | - |
? | |
| acetone + CO2 + ATP + H2O | - |
Rhodomicrobium vannielii RB4 | acetoacetate + AMP + phosphate | - |
? | |
| acetone + CO2 + ATP + H2O | - |
Paracoccus pantotrophus LMD82.1 | acetoacetate + AMP + phosphate | - |
? | |
| butanone + CO2 + ATP + H2O | - |
Rhodobacter capsulatus | 3-oxopentanoate + AMP + phosphate | - |
? | |
| butanone + CO2 + ATP + H2O | - |
Rhodobacter capsulatus PAS100 | 3-oxopentanoate + AMP + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 70000 + x * 85000, also 60000 Da and small amounts of 35000 Da subunits exist, SDS-PAGE | Rhodobacter capsulatus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Rhodobacter capsulatus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
- |
Rhodobacter capsulatus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Rhodobacter capsulatus | |
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