Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Avidin | complete inhibition, prevented by incubation of avidin with biotin prior to its addition | Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0084 | - |
ATP | - |
Zea mays | |
0.064 | - |
geranoyl-CoA | - |
Zea mays | |
0.58 | - |
HCO3- | - |
Zea mays |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plastid | - |
Zea mays | 9536 | - |
plastid | 84% of GCCase activity is recovered in plastids | Daucus carota | 9536 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | absolute requirement | Zea mays |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
122000 | - |
x * 122000, biotin-containing subunit, SDS-PAGE, Western blot analysis | Zea mays |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + geranoyl-CoA + HCO3- | Zea mays | plays an important role in isoprenoid catabolism | ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA | - |
? | |
ATP + geranoyl-CoA + HCO3- | Glycine max | plays an important role in isoprenoid catabolism | ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA | - |
? | |
ATP + geranoyl-CoA + HCO3- | Daucus carota | plays an important role in isoprenoid catabolism | ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Daucus carota | - |
- |
- |
Glycine max | - |
- |
- |
Zea mays | - |
- |
- |
Purification (Comment) | Organism |
---|---|
184fold, from leaves | Zea mays |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
specific activities in different developing embryos, effect of cotyledon development and cell growth on GCCase activity | Daucus carota |
additional information | - |
specific activities in several tissues of 4 and 15 days old seedlings | Glycine max |
additional information | - |
specific activities in several tissues of 7 days old seedlings | Zea mays |
0.138 | - |
- |
Zea mays |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + geranoyl-CoA + HCO3- | ATP-dependent carboxylation of the methyl group that is proximal to the carboxyl end of geranoyl-CoA | Zea mays | ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA | formation of gamma-carboxygeranoyl-CoA | ? | |
ATP + geranoyl-CoA + HCO3- | ATP-dependent carboxylation of the methyl group that is proximal to the carboxyl end of geranoyl-CoA | Glycine max | ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA | formation of gamma-carboxygeranoyl-CoA | ? | |
ATP + geranoyl-CoA + HCO3- | ATP-dependent carboxylation of the methyl group that is proximal to the carboxyl end of geranoyl-CoA | Daucus carota | ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA | formation of gamma-carboxygeranoyl-CoA | ? | |
ATP + geranoyl-CoA + HCO3- | plays an important role in isoprenoid catabolism | Zea mays | ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA | - |
? | |
ATP + geranoyl-CoA + HCO3- | plays an important role in isoprenoid catabolism | Glycine max | ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA | - |
? | |
ATP + geranoyl-CoA + HCO3- | plays an important role in isoprenoid catabolism | Daucus carota | ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA | - |
? | |
additional information | not: methylcrotonyl-CoA | Zea mays | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 122000, biotin-containing subunit, SDS-PAGE, Western blot analysis | Zea mays |
Synonyms | Comment | Organism |
---|---|---|
GCCase | - |
Zea mays |
GCCase | - |
Glycine max |
GCCase | - |
Daucus carota |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.3 | - |
- |
Zea mays |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
active over a broad pH-range | Zea mays |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
biotin | biotin-dependent enzyme | Zea mays | |
biotin | biotin-dependent enzyme | Glycine max | |
biotin | biotin-dependent enzyme | Daucus carota |