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Literature summary for 6.4.1.5 extracted from
- 3-Methylcrotonyl-CoA and geranyl-CoA carboxylases from Pseudomonas citronellolis (1981), Methods Enzymol., 71, 791-799.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 63000 | - |
4 * 63000 + 4 * 75000, biotin-containing subunits, SDS-PAGE | Pseudomonas citronellolis |
| 75000 | - |
4 * 63000 + 4 * 75000, biotin-containing subunits, SDS-PAGE | Pseudomonas citronellolis |
| 520000 | 580000 | gel filtration, native PAGE | Pseudomonas citronellolis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas citronellolis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas citronellolis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8.38 | - |
- |
Pseudomonas citronellolis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, stable for several months | Pseudomonas citronellolis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 3-methylcrotonyl-CoA + HCO3- | 4-8% of the activity relative to (Z)-geranoyl-CoA | Pseudomonas citronellolis | ? | - |
? |  |
| ATP + ethylcrotonyl-CoA + HCO3- | cis-ethylcrotonyl-CoA and trans-ethylcrotonyl-CoA | Pseudomonas citronellolis | ? | - |
? | |
| ATP + farnesyl-CoA + HCO3- | 36% of the activity relative to (Z)-geranoyl-CoA | Pseudomonas citronellolis | ? | - |
? | |
| ATP + geranoyl-CoA + HCO3- | (Z)-geranoyl-CoA, no activity with (E)-geranoyl-CoA | Pseudomonas citronellolis | ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA | - |
? | |
| additional information | the enzyme utilizes 5-15-carbon acyl-CoA substrates | Pseudomonas citronellolis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| octamer | 4 * 63000 + 4 * 75000, biotin-containing subunits, SDS-PAGE | Pseudomonas citronellolis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
50% loss of activity after 1 min | Pseudomonas citronellolis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| biotin | enzyme contains 1 mol of biotin per 148000-157000 g of protein | Pseudomonas citronellolis | |
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Release 2023.1 (January 2023)
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