General Stability | Organism |
---|---|
MCCase extracted with 0.1 M Tris-HCl pH 8.0 is relatively unstable, enzyme is completely inactive 2-3 days after extraction, changing of pH or addition of bovine serum albumin fails to stabilize the enzyme | Glycine max |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetoacetyl-CoA | - |
Daucus carota | |
acetoacetyl-CoA | - |
Glycine max | |
acetoacetyl-CoA | - |
Hordeum vulgare | |
acetoacetyl-CoA | - |
Pisum sativum | |
acetoacetyl-CoA | - |
Solanum tuberosum | |
acetoacetyl-CoA | - |
Zea mays | |
Li+ | - |
Daucus carota | |
Li+ | - |
Glycine max | |
Li+ | - |
Hordeum vulgare | |
Li+ | - |
Pisum sativum | |
Li+ | - |
Solanum tuberosum | |
Li+ | - |
Zea mays | |
Na+ | - |
Daucus carota | |
Na+ | - |
Glycine max | |
Na+ | - |
Hordeum vulgare | |
Na+ | - |
Pisum sativum | |
Na+ | - |
Solanum tuberosum | |
Na+ | - |
Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | 0.05 | 3-methylcrotonoyl-CoA | pH 8.0, 37°C | Hordeum vulgare | |
0.01 | 0.05 | 3-methylcrotonoyl-CoA | pH 8.0, 37°C | Pisum sativum | |
0.01 | 0.05 | 3-methylcrotonoyl-CoA | pH 8.0, 37°C | Zea mays | |
0.01 | 0.05 | 3-methylcrotonoyl-CoA | pH 8.0, 37°C | Solanum tuberosum | |
0.01 | 0.05 | 3-methylcrotonoyl-CoA | pH 8.0, 37°C | Glycine max | |
0.01 | 0.05 | 3-methylcrotonoyl-CoA | pH 8.0, 37°C | Daucus carota | |
0.02 | - |
ATP | pH 8.0, 37°C | Hordeum vulgare | |
0.02 | - |
ATP | pH 8.0, 37°C | Pisum sativum | |
0.02 | - |
ATP | pH 8.0, 37°C | Zea mays | |
0.02 | - |
ATP | pH 8.0, 37°C | Solanum tuberosum | |
0.02 | - |
ATP | pH 8.0, 37°C | Glycine max | |
0.02 | - |
ATP | pH 8.0, 37°C | Daucus carota | |
0.82 | - |
3-methylcrotonoyl-CoA | pH 8.0, 37°C | Hordeum vulgare | |
0.82 | - |
3-methylcrotonoyl-CoA | pH 8.0, 37°C | Pisum sativum | |
0.82 | - |
3-methylcrotonoyl-CoA | pH 8.0, 37°C | Zea mays | |
0.82 | - |
3-methylcrotonoyl-CoA | pH 8.0, 37°C | Solanum tuberosum | |
0.82 | - |
3-methylcrotonoyl-CoA | pH 8.0, 37°C | Glycine max | |
0.82 | - |
3-methylcrotonoyl-CoA | pH 8.0, 37°C | Daucus carota |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Daucus carota | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | requirement for free Mg2+ ions can be partially replaced by Mn2+ or Co2+, but not by Zn2+ | Hordeum vulgare | |
Co2+ | requirement for free Mg2+ ions can be partially replaced by Mn2+ or Co2+, but not by Zn2+ | Pisum sativum | |
Co2+ | requirement for free Mg2+ ions can be partially replaced by Mn2+ or Co2+, but not by Zn2+ | Zea mays | |
Co2+ | requirement for free Mg2+ ions can be partially replaced by Mn2+ or Co2+, but not by Zn2+ | Solanum tuberosum | |
Co2+ | requirement for free Mg2+ ions can be partially replaced by Mn2+ or Co2+, but not by Zn2+ | Glycine max | |
Co2+ | requirement for free Mg2+ ions can be partially replaced by Mn2+ or Co2+, but not by Zn2+ | Daucus carota | |
Cs+ | activator | Hordeum vulgare | |
Cs+ | activator | Pisum sativum | |
Cs+ | activator | Zea mays | |
Cs+ | activator | Solanum tuberosum | |
Cs+ | activator | Glycine max | |
Cs+ | activator | Daucus carota | |
K+ | activator | Hordeum vulgare | |
K+ | activator | Pisum sativum | |
K+ | activator | Zea mays | |
K+ | activator | Solanum tuberosum | |
K+ | activator | Glycine max | |
K+ | activator | Daucus carota | |
Mg2+ | requires free Mg2+ ions for activation, in excess of thatrequired to complex ATP | Hordeum vulgare | |
Mg2+ | requires free Mg2+ ions for activation, in excess of thatrequired to complex ATP | Pisum sativum | |
Mg2+ | requires free Mg2+ ions for activation, in excess of thatrequired to complex ATP | Zea mays | |
Mg2+ | requires free Mg2+ ions for activation, in excess of thatrequired to complex ATP | Solanum tuberosum | |
Mg2+ | requires free Mg2+ ions for activation, in excess of thatrequired to complex ATP | Glycine max | |
Mg2+ | requires free Mg2+ ions for activation, in excess of thatrequired to complex ATP | Daucus carota | |
Mn2+ | requirement for free Mg2+ ions can be partially replaced by Mn2+ or Co2+, but not by Zn2+ | Hordeum vulgare | |
Mn2+ | requirement for free Mg2+ ions can be partially replaced by Mn2+ or Co2+, but not by Zn2+ | Pisum sativum | |
Mn2+ | requirement for free Mg2+ ions can be partially replaced by Mn2+ or Co2+, but not by Zn2+ | Zea mays | |
Mn2+ | requirement for free Mg2+ ions can be partially replaced by Mn2+ or Co2+, but not by Zn2+ | Solanum tuberosum | |
Mn2+ | requirement for free Mg2+ ions can be partially replaced by Mn2+ or Co2+, but not by Zn2+ | Glycine max | |
Mn2+ | requirement for free Mg2+ ions can be partially replaced by Mn2+ or Co2+, but not by Zn2+ | Daucus carota | |
NH4+ | activator | Hordeum vulgare | |
NH4+ | activator | Pisum sativum | |
NH4+ | activator | Zea mays | |
NH4+ | activator | Solanum tuberosum | |
NH4+ | activator | Glycine max | |
NH4+ | activator | Daucus carota | |
Rb+ | activator | Hordeum vulgare | |
Rb+ | activator | Pisum sativum | |
Rb+ | activator | Zea mays | |
Rb+ | activator | Solanum tuberosum | |
Rb+ | activator | Glycine max | |
Rb+ | activator | Daucus carota |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
4 * 80000 + 4 * 60000, SDS-PAGE | Pisum sativum |
60000 | - |
4 * 80000 + 4 * 60000, SDS-PAGE | Solanum tuberosum |
60000 | - |
6 * 800000 + 6 * 60000 | Zea mays |
60000 | - |
6 * 800000 + 6 * 60000 | Glycine max |
60000 | - |
6 * 800000 + 6 * 60000 | Daucus carota |
60000 | - |
6 * 800000 + 6 * 60000 | Solanum lycopersicum |
80000 | - |
4 * 80000 + 4 * 60000, SDS-PAGE | Pisum sativum |
80000 | - |
4 * 80000 + 4 * 60000, SDS-PAGE | Solanum tuberosum |
500000 | - |
gel filtration | Pisum sativum |
500000 | - |
gel filtration | Solanum tuberosum |
800000 | - |
6 * 800000 + 6 * 60000 | Zea mays |
800000 | - |
6 * 800000 + 6 * 60000 | Glycine max |
800000 | - |
6 * 800000 + 6 * 60000 | Daucus carota |
800000 | - |
6 * 800000 + 6 * 60000 | Solanum lycopersicum |
800000 | 900000 | nondenaturing PAGE | Zea mays |
800000 | 900000 | nondenaturing PAGE | Glycine max |
800000 | 900000 | nondenaturing PAGE | Daucus carota |
800000 | 900000 | nondenaturing PAGE | Solanum lycopersicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-methylcrotonyl-CoA + HCO3- | Hordeum vulgare | in addition to leucine catabolism required for the operation of the mevalonate shunt | ADP + phosphate + 3-methylglutaconyl-CoA | - |
ir | |
ATP + 3-methylcrotonyl-CoA + HCO3- | Pisum sativum | in addition to leucine catabolism required for the operation of the mevalonate shunt | ADP + phosphate + 3-methylglutaconyl-CoA | - |
ir | |
ATP + 3-methylcrotonyl-CoA + HCO3- | Zea mays | in addition to leucine catabolism required for the operation of the mevalonate shunt | ADP + phosphate + 3-methylglutaconyl-CoA | - |
ir | |
ATP + 3-methylcrotonyl-CoA + HCO3- | Solanum tuberosum | in addition to leucine catabolism required for the operation of the mevalonate shunt | ADP + phosphate + 3-methylglutaconyl-CoA | - |
ir | |
ATP + 3-methylcrotonyl-CoA + HCO3- | Glycine max | in addition to leucine catabolism required for the operation of the mevalonate shunt | ADP + phosphate + 3-methylglutaconyl-CoA | - |
ir | |
ATP + 3-methylcrotonyl-CoA + HCO3- | Daucus carota | in addition to leucine catabolism required for the operation of the mevalonate shunt | ADP + phosphate + 3-methylglutaconyl-CoA | - |
ir | |
ATP + 3-methylcrotonyl-CoA + HCO3- | Solanum lycopersicum | in addition to leucine catabolism required for the operation of the mevalonate shunt | ADP + phosphate + 3-methylglutaconyl-CoA | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Daucus carota | - |
carrot | - |
Glycine max | - |
soybean, cv. Corsoy 79 | - |
Hordeum vulgare | - |
barley | - |
Pisum sativum | - |
pea | - |
Solanum lycopersicum | - |
tomato | - |
Solanum tuberosum | - |
potato | - |
Zea mays | - |
maize | - |
Purification (Comment) | Organism |
---|---|
- |
Hordeum vulgare |
- |
Pisum sativum |
- |
Zea mays |
- |
Solanum tuberosum |
- |
Glycine max |
- |
Daucus carota |
- |
Solanum lycopersicum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.2062 | - |
- |
Zea mays |
0.3367 | - |
- |
Glycine max |
Storage Stability | Organism |
---|---|
-20°C, purified rnzyme can be stored up to 6 months with minimal loss of activity | Zea mays |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-methylcrotonyl-CoA + HCO3- | - |
Hordeum vulgare | ADP + phosphate + 3-methylglutaconyl-CoA | - |
ir | |
ATP + 3-methylcrotonyl-CoA + HCO3- | - |
Pisum sativum | ADP + phosphate + 3-methylglutaconyl-CoA | - |
ir | |
ATP + 3-methylcrotonyl-CoA + HCO3- | - |
Zea mays | ADP + phosphate + 3-methylglutaconyl-CoA | - |
ir | |
ATP + 3-methylcrotonyl-CoA + HCO3- | - |
Solanum tuberosum | ADP + phosphate + 3-methylglutaconyl-CoA | - |
ir | |
ATP + 3-methylcrotonyl-CoA + HCO3- | - |
Glycine max | ADP + phosphate + 3-methylglutaconyl-CoA | - |
ir | |
ATP + 3-methylcrotonyl-CoA + HCO3- | - |
Daucus carota | ADP + phosphate + 3-methylglutaconyl-CoA | - |
ir | |
ATP + 3-methylcrotonyl-CoA + HCO3- | - |
Solanum lycopersicum | ADP + phosphate + 3-methylglutaconyl-CoA | - |
ir | |
ATP + 3-methylcrotonyl-CoA + HCO3- | in addition to leucine catabolism required for the operation of the mevalonate shunt | Hordeum vulgare | ADP + phosphate + 3-methylglutaconyl-CoA | - |
ir | |
ATP + 3-methylcrotonyl-CoA + HCO3- | in addition to leucine catabolism required for the operation of the mevalonate shunt | Pisum sativum | ADP + phosphate + 3-methylglutaconyl-CoA | - |
ir | |
ATP + 3-methylcrotonyl-CoA + HCO3- | in addition to leucine catabolism required for the operation of the mevalonate shunt | Zea mays | ADP + phosphate + 3-methylglutaconyl-CoA | - |
ir | |
ATP + 3-methylcrotonyl-CoA + HCO3- | in addition to leucine catabolism required for the operation of the mevalonate shunt | Solanum tuberosum | ADP + phosphate + 3-methylglutaconyl-CoA | - |
ir | |
ATP + 3-methylcrotonyl-CoA + HCO3- | in addition to leucine catabolism required for the operation of the mevalonate shunt | Glycine max | ADP + phosphate + 3-methylglutaconyl-CoA | - |
ir | |
ATP + 3-methylcrotonyl-CoA + HCO3- | in addition to leucine catabolism required for the operation of the mevalonate shunt | Daucus carota | ADP + phosphate + 3-methylglutaconyl-CoA | - |
ir | |
ATP + 3-methylcrotonyl-CoA + HCO3- | in addition to leucine catabolism required for the operation of the mevalonate shunt | Solanum lycopersicum | ADP + phosphate + 3-methylglutaconyl-CoA | - |
ir | |
ATP + crotonyl-CoA + HCO3- | much poorer substrate | Hordeum vulgare | ADP + phosphate + glutaconyl-CoA | - |
ir | |
ATP + crotonyl-CoA + HCO3- | much poorer substrate | Pisum sativum | ADP + phosphate + glutaconyl-CoA | - |
ir | |
ATP + crotonyl-CoA + HCO3- | much poorer substrate | Zea mays | ADP + phosphate + glutaconyl-CoA | - |
ir | |
ATP + crotonyl-CoA + HCO3- | much poorer substrate | Solanum tuberosum | ADP + phosphate + glutaconyl-CoA | - |
ir | |
ATP + crotonyl-CoA + HCO3- | much poorer substrate | Glycine max | ADP + phosphate + glutaconyl-CoA | - |
ir | |
ATP + crotonyl-CoA + HCO3- | much poorer substrate | Daucus carota | ADP + phosphate + glutaconyl-CoA | - |
ir | |
additional information | acetoacetyl-CoA is no substrate | Hordeum vulgare | ? | - |
? | |
additional information | acetoacetyl-CoA is no substrate | Pisum sativum | ? | - |
? | |
additional information | acetoacetyl-CoA is no substrate | Zea mays | ? | - |
? | |
additional information | acetoacetyl-CoA is no substrate | Solanum tuberosum | ? | - |
? | |
additional information | acetoacetyl-CoA is no substrate | Glycine max | ? | - |
? | |
additional information | acetoacetyl-CoA is no substrate | Daucus carota | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dodecamer | 6 * 800000 + 6 * 60000 | Zea mays |
dodecamer | 6 * 800000 + 6 * 60000 | Glycine max |
dodecamer | 6 * 800000 + 6 * 60000 | Daucus carota |
dodecamer | 6 * 800000 + 6 * 60000 | Solanum lycopersicum |
octamer | 4 * 80000 + 4 * 60000, SDS-PAGE | Pisum sativum |
octamer | 4 * 80000 + 4 * 60000, SDS-PAGE | Solanum tuberosum |
Synonyms | Comment | Organism |
---|---|---|
3-methylcrotonyl-CoA carboxylase | - |
Hordeum vulgare |
3-methylcrotonyl-CoA carboxylase | - |
Pisum sativum |
3-methylcrotonyl-CoA carboxylase | - |
Zea mays |
3-methylcrotonyl-CoA carboxylase | - |
Solanum tuberosum |
3-methylcrotonyl-CoA carboxylase | - |
Glycine max |
3-methylcrotonyl-CoA carboxylase | - |
Daucus carota |
3-methylcrotonyl-CoA carboxylase | - |
Solanum lycopersicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | - |
Hordeum vulgare |
8 | 8.5 | - |
Pisum sativum |
8 | 8.5 | - |
Zea mays |
8 | 8.5 | - |
Solanum tuberosum |
8 | 8.5 | - |
Glycine max |
8 | 8.5 | - |
Daucus carota |