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Literature summary for 6.4.1.4 extracted from

  • Wurtele, E.S.; Nikolau, B.J.
    Characterization of 3-methylcrotonyl-CoA carboxylase from plants (2000), Methods Enzymol., 324, 280-292.
    View publication on PubMed

General Stability

General Stability Organism
MCCase extracted with 0.1 M Tris-HCl pH 8.0 is relatively unstable, enzyme is completely inactive 2-3 days after extraction, changing of pH or addition of bovine serum albumin fails to stabilize the enzyme Glycine max

Inhibitors

Inhibitors Comment Organism Structure
acetoacetyl-CoA
-
Daucus carota
acetoacetyl-CoA
-
Glycine max
acetoacetyl-CoA
-
Hordeum vulgare
acetoacetyl-CoA
-
Pisum sativum
acetoacetyl-CoA
-
Solanum tuberosum
acetoacetyl-CoA
-
Zea mays
Li+
-
Daucus carota
Li+
-
Glycine max
Li+
-
Hordeum vulgare
Li+
-
Pisum sativum
Li+
-
Solanum tuberosum
Li+
-
Zea mays
Na+
-
Daucus carota
Na+
-
Glycine max
Na+
-
Hordeum vulgare
Na+
-
Pisum sativum
Na+
-
Solanum tuberosum
Na+
-
Zea mays

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01 0.05 3-methylcrotonoyl-CoA pH 8.0, 37°C Hordeum vulgare
0.01 0.05 3-methylcrotonoyl-CoA pH 8.0, 37°C Pisum sativum
0.01 0.05 3-methylcrotonoyl-CoA pH 8.0, 37°C Zea mays
0.01 0.05 3-methylcrotonoyl-CoA pH 8.0, 37°C Solanum tuberosum
0.01 0.05 3-methylcrotonoyl-CoA pH 8.0, 37°C Glycine max
0.01 0.05 3-methylcrotonoyl-CoA pH 8.0, 37°C Daucus carota
0.02
-
ATP pH 8.0, 37°C Hordeum vulgare
0.02
-
ATP pH 8.0, 37°C Pisum sativum
0.02
-
ATP pH 8.0, 37°C Zea mays
0.02
-
ATP pH 8.0, 37°C Solanum tuberosum
0.02
-
ATP pH 8.0, 37°C Glycine max
0.02
-
ATP pH 8.0, 37°C Daucus carota
0.82
-
3-methylcrotonoyl-CoA pH 8.0, 37°C Hordeum vulgare
0.82
-
3-methylcrotonoyl-CoA pH 8.0, 37°C Pisum sativum
0.82
-
3-methylcrotonoyl-CoA pH 8.0, 37°C Zea mays
0.82
-
3-methylcrotonoyl-CoA pH 8.0, 37°C Solanum tuberosum
0.82
-
3-methylcrotonoyl-CoA pH 8.0, 37°C Glycine max
0.82
-
3-methylcrotonoyl-CoA pH 8.0, 37°C Daucus carota

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Daucus carota 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ requirement for free Mg2+ ions can be partially replaced by Mn2+ or Co2+, but not by Zn2+ Hordeum vulgare
Co2+ requirement for free Mg2+ ions can be partially replaced by Mn2+ or Co2+, but not by Zn2+ Pisum sativum
Co2+ requirement for free Mg2+ ions can be partially replaced by Mn2+ or Co2+, but not by Zn2+ Zea mays
Co2+ requirement for free Mg2+ ions can be partially replaced by Mn2+ or Co2+, but not by Zn2+ Solanum tuberosum
Co2+ requirement for free Mg2+ ions can be partially replaced by Mn2+ or Co2+, but not by Zn2+ Glycine max
Co2+ requirement for free Mg2+ ions can be partially replaced by Mn2+ or Co2+, but not by Zn2+ Daucus carota
Cs+ activator Hordeum vulgare
Cs+ activator Pisum sativum
Cs+ activator Zea mays
Cs+ activator Solanum tuberosum
Cs+ activator Glycine max
Cs+ activator Daucus carota
K+ activator Hordeum vulgare
K+ activator Pisum sativum
K+ activator Zea mays
K+ activator Solanum tuberosum
K+ activator Glycine max
K+ activator Daucus carota
Mg2+ requires free Mg2+ ions for activation, in excess of thatrequired to complex ATP Hordeum vulgare
Mg2+ requires free Mg2+ ions for activation, in excess of thatrequired to complex ATP Pisum sativum
Mg2+ requires free Mg2+ ions for activation, in excess of thatrequired to complex ATP Zea mays
Mg2+ requires free Mg2+ ions for activation, in excess of thatrequired to complex ATP Solanum tuberosum
Mg2+ requires free Mg2+ ions for activation, in excess of thatrequired to complex ATP Glycine max
Mg2+ requires free Mg2+ ions for activation, in excess of thatrequired to complex ATP Daucus carota
Mn2+ requirement for free Mg2+ ions can be partially replaced by Mn2+ or Co2+, but not by Zn2+ Hordeum vulgare
Mn2+ requirement for free Mg2+ ions can be partially replaced by Mn2+ or Co2+, but not by Zn2+ Pisum sativum
Mn2+ requirement for free Mg2+ ions can be partially replaced by Mn2+ or Co2+, but not by Zn2+ Zea mays
Mn2+ requirement for free Mg2+ ions can be partially replaced by Mn2+ or Co2+, but not by Zn2+ Solanum tuberosum
Mn2+ requirement for free Mg2+ ions can be partially replaced by Mn2+ or Co2+, but not by Zn2+ Glycine max
Mn2+ requirement for free Mg2+ ions can be partially replaced by Mn2+ or Co2+, but not by Zn2+ Daucus carota
NH4+ activator Hordeum vulgare
NH4+ activator Pisum sativum
NH4+ activator Zea mays
NH4+ activator Solanum tuberosum
NH4+ activator Glycine max
NH4+ activator Daucus carota
Rb+ activator Hordeum vulgare
Rb+ activator Pisum sativum
Rb+ activator Zea mays
Rb+ activator Solanum tuberosum
Rb+ activator Glycine max
Rb+ activator Daucus carota

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
4 * 80000 + 4 * 60000, SDS-PAGE Pisum sativum
60000
-
4 * 80000 + 4 * 60000, SDS-PAGE Solanum tuberosum
60000
-
6 * 800000 + 6 * 60000 Zea mays
60000
-
6 * 800000 + 6 * 60000 Glycine max
60000
-
6 * 800000 + 6 * 60000 Daucus carota
60000
-
6 * 800000 + 6 * 60000 Solanum lycopersicum
80000
-
4 * 80000 + 4 * 60000, SDS-PAGE Pisum sativum
80000
-
4 * 80000 + 4 * 60000, SDS-PAGE Solanum tuberosum
500000
-
gel filtration Pisum sativum
500000
-
gel filtration Solanum tuberosum
800000
-
6 * 800000 + 6 * 60000 Zea mays
800000
-
6 * 800000 + 6 * 60000 Glycine max
800000
-
6 * 800000 + 6 * 60000 Daucus carota
800000
-
6 * 800000 + 6 * 60000 Solanum lycopersicum
800000 900000 nondenaturing PAGE Zea mays
800000 900000 nondenaturing PAGE Glycine max
800000 900000 nondenaturing PAGE Daucus carota
800000 900000 nondenaturing PAGE Solanum lycopersicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 3-methylcrotonyl-CoA + HCO3- Hordeum vulgare in addition to leucine catabolism required for the operation of the mevalonate shunt ADP + phosphate + 3-methylglutaconyl-CoA
-
ir
ATP + 3-methylcrotonyl-CoA + HCO3- Pisum sativum in addition to leucine catabolism required for the operation of the mevalonate shunt ADP + phosphate + 3-methylglutaconyl-CoA
-
ir
ATP + 3-methylcrotonyl-CoA + HCO3- Zea mays in addition to leucine catabolism required for the operation of the mevalonate shunt ADP + phosphate + 3-methylglutaconyl-CoA
-
ir
ATP + 3-methylcrotonyl-CoA + HCO3- Solanum tuberosum in addition to leucine catabolism required for the operation of the mevalonate shunt ADP + phosphate + 3-methylglutaconyl-CoA
-
ir
ATP + 3-methylcrotonyl-CoA + HCO3- Glycine max in addition to leucine catabolism required for the operation of the mevalonate shunt ADP + phosphate + 3-methylglutaconyl-CoA
-
ir
ATP + 3-methylcrotonyl-CoA + HCO3- Daucus carota in addition to leucine catabolism required for the operation of the mevalonate shunt ADP + phosphate + 3-methylglutaconyl-CoA
-
ir
ATP + 3-methylcrotonyl-CoA + HCO3- Solanum lycopersicum in addition to leucine catabolism required for the operation of the mevalonate shunt ADP + phosphate + 3-methylglutaconyl-CoA
-
ir

Organism

Organism UniProt Comment Textmining
Daucus carota
-
carrot
-
Glycine max
-
soybean, cv. Corsoy 79
-
Hordeum vulgare
-
barley
-
Pisum sativum
-
pea
-
Solanum lycopersicum
-
tomato
-
Solanum tuberosum
-
potato
-
Zea mays
-
maize
-

Purification (Commentary)

Purification (Comment) Organism
-
Hordeum vulgare
-
Pisum sativum
-
Zea mays
-
Solanum tuberosum
-
Glycine max
-
Daucus carota
-
Solanum lycopersicum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.2062
-
-
Zea mays
0.3367
-
-
Glycine max

Storage Stability

Storage Stability Organism
-20°C, purified rnzyme can be stored up to 6 months with minimal loss of activity Zea mays

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 3-methylcrotonyl-CoA + HCO3-
-
Hordeum vulgare ADP + phosphate + 3-methylglutaconyl-CoA
-
ir
ATP + 3-methylcrotonyl-CoA + HCO3-
-
Pisum sativum ADP + phosphate + 3-methylglutaconyl-CoA
-
ir
ATP + 3-methylcrotonyl-CoA + HCO3-
-
Zea mays ADP + phosphate + 3-methylglutaconyl-CoA
-
ir
ATP + 3-methylcrotonyl-CoA + HCO3-
-
Solanum tuberosum ADP + phosphate + 3-methylglutaconyl-CoA
-
ir
ATP + 3-methylcrotonyl-CoA + HCO3-
-
Glycine max ADP + phosphate + 3-methylglutaconyl-CoA
-
ir
ATP + 3-methylcrotonyl-CoA + HCO3-
-
Daucus carota ADP + phosphate + 3-methylglutaconyl-CoA
-
ir
ATP + 3-methylcrotonyl-CoA + HCO3-
-
Solanum lycopersicum ADP + phosphate + 3-methylglutaconyl-CoA
-
ir
ATP + 3-methylcrotonyl-CoA + HCO3- in addition to leucine catabolism required for the operation of the mevalonate shunt Hordeum vulgare ADP + phosphate + 3-methylglutaconyl-CoA
-
ir
ATP + 3-methylcrotonyl-CoA + HCO3- in addition to leucine catabolism required for the operation of the mevalonate shunt Pisum sativum ADP + phosphate + 3-methylglutaconyl-CoA
-
ir
ATP + 3-methylcrotonyl-CoA + HCO3- in addition to leucine catabolism required for the operation of the mevalonate shunt Zea mays ADP + phosphate + 3-methylglutaconyl-CoA
-
ir
ATP + 3-methylcrotonyl-CoA + HCO3- in addition to leucine catabolism required for the operation of the mevalonate shunt Solanum tuberosum ADP + phosphate + 3-methylglutaconyl-CoA
-
ir
ATP + 3-methylcrotonyl-CoA + HCO3- in addition to leucine catabolism required for the operation of the mevalonate shunt Glycine max ADP + phosphate + 3-methylglutaconyl-CoA
-
ir
ATP + 3-methylcrotonyl-CoA + HCO3- in addition to leucine catabolism required for the operation of the mevalonate shunt Daucus carota ADP + phosphate + 3-methylglutaconyl-CoA
-
ir
ATP + 3-methylcrotonyl-CoA + HCO3- in addition to leucine catabolism required for the operation of the mevalonate shunt Solanum lycopersicum ADP + phosphate + 3-methylglutaconyl-CoA
-
ir
ATP + crotonyl-CoA + HCO3- much poorer substrate Hordeum vulgare ADP + phosphate + glutaconyl-CoA
-
ir
ATP + crotonyl-CoA + HCO3- much poorer substrate Pisum sativum ADP + phosphate + glutaconyl-CoA
-
ir
ATP + crotonyl-CoA + HCO3- much poorer substrate Zea mays ADP + phosphate + glutaconyl-CoA
-
ir
ATP + crotonyl-CoA + HCO3- much poorer substrate Solanum tuberosum ADP + phosphate + glutaconyl-CoA
-
ir
ATP + crotonyl-CoA + HCO3- much poorer substrate Glycine max ADP + phosphate + glutaconyl-CoA
-
ir
ATP + crotonyl-CoA + HCO3- much poorer substrate Daucus carota ADP + phosphate + glutaconyl-CoA
-
ir
additional information acetoacetyl-CoA is no substrate Hordeum vulgare ?
-
?
additional information acetoacetyl-CoA is no substrate Pisum sativum ?
-
?
additional information acetoacetyl-CoA is no substrate Zea mays ?
-
?
additional information acetoacetyl-CoA is no substrate Solanum tuberosum ?
-
?
additional information acetoacetyl-CoA is no substrate Glycine max ?
-
?
additional information acetoacetyl-CoA is no substrate Daucus carota ?
-
?

Subunits

Subunits Comment Organism
dodecamer 6 * 800000 + 6 * 60000 Zea mays
dodecamer 6 * 800000 + 6 * 60000 Glycine max
dodecamer 6 * 800000 + 6 * 60000 Daucus carota
dodecamer 6 * 800000 + 6 * 60000 Solanum lycopersicum
octamer 4 * 80000 + 4 * 60000, SDS-PAGE Pisum sativum
octamer 4 * 80000 + 4 * 60000, SDS-PAGE Solanum tuberosum

Synonyms

Synonyms Comment Organism
3-methylcrotonyl-CoA carboxylase
-
Hordeum vulgare
3-methylcrotonyl-CoA carboxylase
-
Pisum sativum
3-methylcrotonyl-CoA carboxylase
-
Zea mays
3-methylcrotonyl-CoA carboxylase
-
Solanum tuberosum
3-methylcrotonyl-CoA carboxylase
-
Glycine max
3-methylcrotonyl-CoA carboxylase
-
Daucus carota
3-methylcrotonyl-CoA carboxylase
-
Solanum lycopersicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5
-
Hordeum vulgare
8 8.5
-
Pisum sativum
8 8.5
-
Zea mays
8 8.5
-
Solanum tuberosum
8 8.5
-
Glycine max
8 8.5
-
Daucus carota