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Literature summary for 6.4.1.4 extracted from
- Methylcrotonyl-CoA carboxylase from barley (Hordeum vulgare). Purification, kinetic characterization, and senescence-related induction of activity (1998), J. Plant Physiol., 152, 213-221.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | activity is stimulated by continuous darkness | Hordeum vulgare |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetoacetyl-CoA | 95% inhibition | Hordeum vulgare |  |
| palmitoyl-CoA | 25% inhibition | Hordeum vulgare | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.8 | - |
bicarbonate | experimental conditions not available | Hordeum vulgare | |
| 21 | - |
ATP | experimental conditions not available | Hordeum vulgare | |
| 31 | - |
3-methylcrotonoyl-CoA | experimental conditions not available | Hordeum vulgare | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | carboxylation of methylcrotonyl-CoA is Mg2+-dependent | Hordeum vulgare | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
1 * 60000 + 1 * 85000 | Hordeum vulgare |
| 85000 | - |
1 * 60000 + 1 * 85000 | Hordeum vulgare |
| 660000 | - |
- |
Hordeum vulgare |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hordeum vulgare | - |
barley | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Hordeum vulgare |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 3-methylcrotonyl-CoA + HCO3- | - |
Hordeum vulgare | ADP + phosphate + 3-methylglutaconyl-CoA | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterodimer | 1 * 60000 + 1 * 85000 | Hordeum vulgare |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| methylcrotonyl-CoA carboxylase | - |
Hordeum vulgare |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Hordeum vulgare |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 8.3 | - |
Hordeum vulgare |
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Release 2023.1 (January 2023)
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