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Literature summary for 6.4.1.4 extracted from
- 3-Methylcrotonyl-CoA carboxylase from bovine kidney. EC 6.4.1.4. 3-Methylcrotonyl-CoA: carbon dioxide ligase (ADP-forming) (1981), Methods Enzymol., 71, 800-804.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.078 | - |
3-methylcrotonoyl-CoA | kidney enzyme | Bos taurus |  |
| 0.082 | - |
ATP | kidney enzyme | Bos taurus | |
| 1.8 | - |
HCO3- | kidney enzyme | Bos taurus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrial inner membrane | - |
Bos taurus | 5743 | - |
| mitochondrion | - |
Bos taurus | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | activated 4 to 5fold by K+ or NH4+, 50-100 mM, kidney enzyme | Bos taurus | |
| NH4+ | activated 4 to 5fold by K+ or NH4+, 50-100 mM, kidney enzyme | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 835000 | - |
PAGE, gel filtration | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Homo sapiens | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| kidney enzyme | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adrenal gland | - |
Bos taurus | - |
| kidney | - |
Rattus norvegicus | - |
| kidney | - |
Bos taurus | - |
| liver | - |
Rattus norvegicus | - |
| liver | - |
Bos taurus | - |
| lung | - |
Bos taurus | - |
| placenta | - |
Homo sapiens | - |
| skeletal muscle | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.84 | - |
kidney enzyme | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 3-methylcrotonoyl-CoA + HCO3- | - |
Homo sapiens | ADP + phosphate + 3-methylglutaconyl-CoA | - |
? | |
| ATP + 3-methylcrotonoyl-CoA + HCO3- | - |
Rattus norvegicus | ADP + phosphate + 3-methylglutaconyl-CoA | - |
? | |
| ATP + 3-methylcrotonoyl-CoA + HCO3- | - |
Bos taurus | ADP + phosphate + 3-methylglutaconyl-CoA | - |
? | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| biotin | enzyme contains biotin | Homo sapiens | |
| biotin | enzyme contains biotin | Rattus norvegicus | |
| biotin | enzyme contains biotin | Bos taurus | |
| biotin | contains 1 mol biotin per 157000 MW AB protomeric structure | Bos taurus | |
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Release 2023.1 (January 2023)
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