Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Leptospira interrogans |
Protein Variants | Comment | Organism |
---|---|---|
A431C | kcat (propionyl-CoA) decreased compared to wild-type, Km (propionyl-CoA) increased compared to wild-type. kcat (acetyl-CoA) increased compared to wild-type, Km (propionyl-CoA) similar to wild-type | Leptospira interrogans |
A431D | mutant enzyme shows no activity | Leptospira interrogans |
A431I | kcat (propionyl-CoA) decreased compared to wild-type, Km (propionyl-CoA) increased compared to wild-type. kcat (acetyl-CoA) increased compared to wild-type, Km (propionyl-CoA) similar to wild-type | Leptospira interrogans |
A431L | mutant enzyme shows no activity | Leptospira interrogans |
Y430H/A431I | mutant enzyme shows no activity | Leptospira interrogans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | 1 | propionyl-CoA | pH 8, 30°C, wild-type | Leptospira interrogans | |
0.24 | - |
propionyl-CoA | pH 8, 30°C, mutant A431C | Leptospira interrogans | |
1.3 | - |
propionyl-CoA | pH 8, 30°C, mutant A431I | Leptospira interrogans | |
4.6 | - |
acetyl-CoA | pH 8, 30°C, wild-type | Leptospira interrogans | |
4.8 | - |
acetyl-CoA | pH 8, 30°C, mutant A431C | Leptospira interrogans | |
5 | - |
acetyl-CoA | pH 8, 30°C, mutant A431I | Leptospira interrogans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
669000 | - |
alpha4beta4 quaternary structure | Leptospira interrogans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leptospira interrogans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Leptospira interrogans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetyl-CoA + HCO3- | - |
Leptospira interrogans | ADP + phosphate + malonyl-CoA | - |
? | |
ATP + propionyl-CoA + HCO3- | preferred substrate | Leptospira interrogans | ADP + phosphate + (S)-methylmalonyl-CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LA_2736-LA_2735 | - |
Leptospira interrogans |
PCC | - |
Leptospira interrogans |
pccBC | - |
Leptospira interrogans |
Propionyl-CoA carboxylase | - |
Leptospira interrogans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Leptospira interrogans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.47 | - |
propionyl-CoA | pH 8, 30°C, mutant A431I | Leptospira interrogans | |
0.66 | - |
acetyl-CoA | pH 8, 30°C, wild-type | Leptospira interrogans | |
1.4 | - |
acetyl-CoA | pH 8, 30°C, mutant A431I | Leptospira interrogans | |
1.46 | - |
propionyl-CoA | pH 8, 30°C, mutant A431C | Leptospira interrogans | |
2.86 | - |
propionyl-CoA | pH 8, 30°C, wild-type | Leptospira interrogans | |
4 | - |
acetyl-CoA | pH 8, 30°C, mutant A431C | Leptospira interrogans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Leptospira interrogans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.144 | - |
acetyl-CoA | pH 8, 30°C, wild-type | Leptospira interrogans | |
0.274 | - |
acetyl-CoA | pH 8, 30°C, mutant A431I | Leptospira interrogans | |
0.367 | - |
propionyl-CoA | pH 8, 30°C, mutant A431I | Leptospira interrogans | |
0.826 | - |
acetyl-CoA | pH 8, 30°C, mutant A431C | Leptospira interrogans | |
6.09 | - |
propionyl-CoA | pH 8, 30°C, mutant A431C | Leptospira interrogans | |
26 | - |
propionyl-CoA | pH 8, 30°C, wild-type | Leptospira interrogans |