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Literature summary for 6.4.1.2 extracted from

  • Chuakrut, S.; Arai, H.; Ishii, M.; Igarashi, Y.
    Characterization of a bifunctional archaeal acyl coenzyme A carboxylase (2003), J. Bacteriol., 185, 938-947 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information NADP+, NADPH, 3-hydroxypropionate, succinate, fumarate, malate, and glyoxylate have no significant effect on acetyl-CoA carboxylase or propionyl-CoA carboxylase activities Acidianus brierleyi
palmitoyl-CoA 0.5 mM palmitoyl-CoA has a slight stimulative effect on acetyl-CoA carboxylase (116%) and propionyl-CoA carboxylase (135%) activities Acidianus brierleyi

Inhibitors

Inhibitors Comment Organism Structure
ATP an excess of ATP (2.0 mM) has an inhibitory effect on the enzymatic activities Acidianus brierleyi
citrate citrate inhibits the enzyme activity by more than 50% at concentrations of 3 mM Acidianus brierleyi
Co2+ 26% residual activity at 10-15 mM Acidianus brierleyi
CoASH inhibits acetyl-CoA carboxylase activity 50 to 57% and propionyl-CoA carboxylase activity 11 to 13% Acidianus brierleyi
dithiothreitol dithiothreitol inhibits the enzyme slightly, by approximately 12%, at a final concentration of 5 to 10 mM Acidianus brierleyi
K+ the enzyme shows less than 50% activity when the concentration is higher than 40 mM Acidianus brierleyi
malonyl-CoA inhibits acetyl-CoA carboxylase activity 50 to 57% and propionyl-CoA carboxylase activity 11 to 13% Acidianus brierleyi
methylmalonyl-CoA 60% inhibition at 0.5 mM; 79% inhibition at 0.5 mM Acidianus brierleyi
Mg2+ an excess of Mg2+ (higher than 4.0 mM) has an inhibitory effect on the enzymatic activities Acidianus brierleyi
Mn2+ an excess of Mn2+ (higher than 2.0 mM) has an inhibitory effect on the enzymatic activities Acidianus brierleyi
Na+ the enzyme shows less than 50% activity when the concentration is higher than 40 mM Acidianus brierleyi
succinyl-CoA 57% inhibition at 0.5 mM; 84% inhibition at 0.5 mM Acidianus brierleyi
sulfate sulfate inhibits the enzyme activity by more than 50% at concentrations of 25 mM Acidianus brierleyi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
ATP with acetyl-CoA as cosubstrate, at pH 7.5 and 70°C Acidianus brierleyi
0.05
-
ATP with propionyl-CoA as cosubstrate, at pH 7.5 and 70°C Acidianus brierleyi
0.1
-
propionyl-CoA at pH 7.5 and 70°C Acidianus brierleyi
0.17
-
acetyl-CoA at pH 7.5 and 70°C Acidianus brierleyi

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, maximum activity at 4 mM Acidianus brierleyi
additional information Fe2+, Cu2+, Ca2+, and Ni2+ (0.5 to 20 mM) have no effect on enzyme activity Acidianus brierleyi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
540000
-
gel filtration Acidianus brierleyi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + acetyl-CoA + HCO3- Acidianus brierleyi
-
ADP + phosphate + malonyl-CoA
-
?
ATP + propionyl-CoA + HCO3- Acidianus brierleyi
-
ADP + phosphate + methylmalonyl-CoA
-
?

Organism

Organism UniProt Comment Textmining
Acidianus brierleyi Q877I4 and Q877I5 and Q877I3
-
-

Purification (Commentary)

Purification (Comment) Organism
Streptavidin column chromatography and Superose 6 gel filtration Acidianus brierleyi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + acetyl-CoA + HCO3-
-
Acidianus brierleyi ADP + phosphate + malonyl-CoA
-
?
ATP + propionyl-CoA + HCO3-
-
Acidianus brierleyi ADP + phosphate + methylmalonyl-CoA
-
?
additional information neither succinyl-CoA, palmitoyl-CoA, acetate (plus 0.5 mM CoASH), propionate (plus 0.5 mM CoASH), nor pyruvate is carboxylated by the enzyme Acidianus brierleyi ?
-
?
n-butyryl-CoA + HCO3- 4.6% activity compared to acetyl-CoA or propionyl-CoA Acidianus brierleyi ?
-
?

Subunits

Subunits Comment Organism
homotrimer 4 * 62000 + 4 * 59000 + 4 * 20000, SDS-PAGE Acidianus brierleyi

Synonyms

Synonyms Comment Organism
ACCB
-
Acidianus brierleyi
AccC
-
Acidianus brierleyi
acyl coenzyme A carboxylase
-
Acidianus brierleyi
acyl-CoA carboxylase
-
Acidianus brierleyi
PccB
-
Acidianus brierleyi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60 70
-
Acidianus brierleyi

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 90 the activity of the enzyme gradually decreases at 50°C, with a residual activity of around 60% after 2 h of incubation. At 60°C, the residual activity of the enzyme is 50% after incubation for 35 to 40 min, whereas the residual activities at 70 and 80°C are less than 15%. The enzyme completely loses its activity within 10 min when it is incubated at 90°C or above Acidianus brierleyi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4 6.9
-
Acidianus brierleyi

Cofactor

Cofactor Comment Organism Structure
ATP the enzyme exhibits the maximum activity when the ATP concentration is 1.0-1.5 mM. Neither ADP, AMP, GTP, CTP, UTP, nor dTTP (0.2 mM) can substitute for ATP Acidianus brierleyi