Activating Compound | Comment | Organism | Structure |
---|---|---|---|
citrate | allosteric activation, presence of citrate leads to dose-dependent formation of dimers and tetramers | Homo sapiens |
Application | Comment | Organism |
---|---|---|
synthesis | expression in Bombyx mori using the silkworm NPV bacmid system. Recombinant protein shows biotinylation capacity,and exhibits posttranslational biotinylation and phosphorylation | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression in Bombyx mori | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | deletion of the N-terminal 148 hydrophobic amino acids to enhance the solubility of heterologous protein expressed in Bomby mori. Expression of the deletion mutant leads to a high yield of recombinant protein | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O00763 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | phosphorylation decreases ACC2 catalytic activity whereas dephosphorylation activates the enzymatic function by 2fold. Recombinant protein expressed in Bombyx mori exhibits posttranslational phosphorylation | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.786 | - |
recombinant N-terminal deletion mutant, pH 7.4, 37°C | Homo sapiens |
1.336 | - |
recombinant N-terminal deletion mutant treated with Lambda protein phosphatase, pH 7.4, 37°C | Homo sapiens |
1.363 | - |
recombinant N-terminal deletion mutant, pH 7.4, 37°C, presence of 5 mM citrate | Homo sapiens |
2.246 | - |
recombinant N-terminal deletion mutant, pH 7.4, 37°C, presence of 10 mM citrate | Homo sapiens |
4.186 | - |
recombinant N-terminal deletion mutant, pH 7.4, 37°C, presence of 20 mM citrate | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetyl-CoA + HCO3- | - |
Homo sapiens | ADP + phosphate + malonyl-CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ACACB | - |
Homo sapiens |
ACC2 | - |
Homo sapiens |