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Literature summary for 6.4.1.2 extracted from

  • Chuakrut, S.; Arai, H.; Ishii, M.; Igarashi, Y.
    Characterization of a bifunctional archaeal acyl coenzyme A carboxylase (2003), J. Bacteriol., 185, 938-947.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.4.1.2

Activating Compound

Activating Compound Comment Organism Structure
biotin biotin-dependent enzyme Acidianus brierleyi
palmitoyl-CoA slight stimulative effect on acetyl-CoA carboxylase (116%) and propionyl-CoA carboxylase (135%) Acidianus brierleyi

Cloned(Commentary)

Cloned (Comment) Organism
-
 Acidianus brierleyi

Inhibitors

Inhibitors Comment Organism Structure
ATP above 2.0 mM Acidianus brierleyi
citrate 3 mM, more than 50% inhibition Acidianus brierleyi
CoA 1 mM, 11-13% inhibition of acetyl-CoA carboxylase Acidianus brierleyi
DTT 5-10 mM, 12% inhibition Acidianus brierleyi
K+
-
 Acidianus brierleyi
malonyl-CoA 0.5 mM, 50-57% inhibition of acetyl-CoA carboxylase Acidianus brierleyi
methylmalonyl-CoA 0.5 mM, 79% inhibition of acetyl-CoA carboxylase Acidianus brierleyi
Mg2+ above 4.0 mM Acidianus brierleyi
Mn2+ above 2.0 mM Acidianus brierleyi
Na+
-
 Acidianus brierleyi
succinyl-CoA 0.5 mM, 84% inhibition of acetyl-CoA carboxylase Acidianus brierleyi
sulfate 25 mM, more than 50% inhibition Acidianus brierleyi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17
-
 acetyl-CoA pH 7.5, 70°C Acidianus brierleyi

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 26% of the maximum activity is observed when Co2+ (10 to 15 mM) is used as a divalent cation Acidianus brierleyi
Mg2+ maximum acetyl-CoA carboxylase activity is obtained at 4.0 mM Acidianus brierleyi
Mn2+ 1.0 mM Mn2+ gives 82% of the maximum activity obtained with the Mg2+ ion Acidianus brierleyi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
 4 * 62000 + 4 * 59000 + 4 * 20000, SDS-PAGE Acidianus brierleyi
59000
-
 4 * 62000 + 4 * 59000 + 4 * 20000, SDS-PAGE Acidianus brierleyi
62000
-
 4 * 62000 + 4 * 59000 + 4 * 20000, SDS-PAGE Acidianus brierleyi
540000
-
-
 Acidianus brierleyi

Organism

Organism UniProt Comment Textmining
Acidianus brierleyi Q877I4 and Q877I3 and Q877I5 Q877I4: biotin carboxyl carrier protein of acetyl-CoA carboxylase, Q877I3: carboxyltransferase subunit of acetyl-CoA carboxylase, Q877I5: biotin carboxylase subunit of acetyl-CoA carboxylase
-

Purification (Commentary)

Purification (Comment) Organism
-
 Acidianus brierleyi

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
14.5
-
 pH 7.5, 70°C, acetyl-CoA carboxylase activity Acidianus brierleyi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + acetyl-CoA + HCO3- the bifunctional enzyme also catalyzes the carboxylation of propionyl-CoA. Vmax/Km for carboxylation of propanoyl-CoA is 1.6fold higher than Vmax/Km for carboxylation of acetyl-CoA. Quite specific for ATP. No activity with ADP, AMP, GTP, CTP, UTP, or dTTP (0.2 mM) Acidianus brierleyi ADP + phosphate + malonyl-CoA
-
 ?
ATP + acetyl-CoA + HCO3- n-butyryl-CoA (0.5 mM) is carboxylated at 4.6% compared to carboxylation of acetyl-CoA or propionyl-CoA Acidianus brierleyi ?
-
 ?
additional information neither succinyl-CoA, palmitoyl-CoA, acetate (plus 0.5 mM CoASH), propionate (plus 0.5 mM CoASH), nor pyruvate is carboxylated by the enzyme Acidianus brierleyi ?
-
 ?

Subunits

Subunits Comment Organism
dodecamer 4 * 62000 + 4 * 59000 + 4 * 20000, SDS-PAGE Acidianus brierleyi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Acidianus brierleyi
70
-
 assay at Acidianus brierleyi

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 75 50°C: about 60% of maximal activity, 75°C: about 60% of maximal activity Acidianus brierleyi

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 2 h, 40% loss of activity Acidianus brierleyi
60
-
 30 min, 50% loss of activity Acidianus brierleyi
70
-
 30 min, 90% loss of activity Acidianus brierleyi
80
-
 30 min, 95% loss of activity Acidianus brierleyi
90
-
 10 min, complete loss of activity Acidianus brierleyi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4 6.9 at 70°C Acidianus brierleyi
7.5
-
 assay at Acidianus brierleyi