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Literature summary for 6.4.1.2 extracted from

  • Reverdatto, S.; Beilinson, V.; Nielsen, N.C.
    A multisubunit acetyl coenzyme A carboxylase from soybean (1999), Plant Physiol., 119, 961-978.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.4.1.2

Cloned(Commentary)

Cloned (Comment) Organism
DNA of different subunits imported into isolated chloroplast from Pisum sativum, proteins expressed in Escherichia coli accumulate in inclusion bodies Glycine max

Localization

Localization Comment Organism GeneOntology No. Textmining
plastid
-
 Glycine max 9536
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
600000
-
 greater than 600000 Da, gel filtration, complex from alpha and beta subunit of caroxyltransferase Glycine max
800000
-
 greater than 800000 Da, gel filtration, complex from biotin carboxylase and biotin carboxyl carrier protein Glycine max

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + acetyl-CoA + HCO3- Pisum sativum key enzyme of fatty acid biosynthesis ADP + phosphate + malonyl-CoA
-
 ?
ATP + acetyl-CoA + HCO3- Glycine max key enzyme of fatty acid biosynthesis ADP + phosphate + malonyl-CoA
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
guanidine inclusion bodies soluble in 6 M urea Glycine max
urea inclusion bodies soluble in 6 M urea Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max
-
-
-
Pisum sativum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + acetyl-CoA + HCO3-
-
 Pisum sativum ADP + phosphate + malonyl-CoA
-
 ?
ATP + acetyl-CoA + HCO3-
-
 Glycine max ADP + phosphate + malonyl-CoA
-
 ?
ATP + acetyl-CoA + HCO3- key enzyme of fatty acid biosynthesis Pisum sativum ADP + phosphate + malonyl-CoA
-
 ?
ATP + acetyl-CoA + HCO3- key enzyme of fatty acid biosynthesis Glycine max ADP + phosphate + malonyl-CoA
-
 ?

Subunits

Subunits Comment Organism
More multisubunit enzyme consisting of biotin carboxylase, biotin carboxyl carrier protein and the alpha and beta subunits of carboxyltransferase Pisum sativum
More multisubunit enzyme consisting of biotin carboxylase, biotin carboxyl carrier protein and the alpha and beta subunits of carboxyltransferase, protein dissociates into two complexes in presence of 0.5 M KCl, when subunit complexes of biotin carboxylase and biotin carboxyl carrier protein and alpha and beta subunits of carboxyltransferase are expressed separately the activity of full size protein can be achieved by mixing the enzyme subunit complexes Glycine max

Cofactor

Cofactor Comment Organism Structure
biotin enzyme-bound Pisum sativum
biotin enzyme-bound Glycine max