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Literature summary for 6.4.1.2 extracted from
- Purification and characterization of acetyl-CoA carboxylase from diatom Cyclotella cryptica (1990), Plant Physiol., 92, 73-78.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| citrate | no effect | Cyclotella cryptica |  |
General Stability
| General Stability | Organism |
|---|---|
| citrate, slightly stabilizes | Cyclotella cryptica |
| NaCl or KCl required for stability | Cyclotella cryptica |
| sulfhydryl reducing agent, e.g. DTT required for stability | Cyclotella cryptica |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Cyclotella cryptica | |
| malonyl-CoA | - |
Cyclotella cryptica | |
| palmitate | - |
Cyclotella cryptica | |
| palmitoyl-CoA | - |
Cyclotella cryptica | |
| phosphate | - |
Cyclotella cryptica | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.065 | - |
ATP | - |
Cyclotella cryptica | |
| 0.233 | - |
acetyl-CoA | - |
Cyclotella cryptica | |
| 0.75 | - |
HCO3- | - |
Cyclotella cryptica | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | maximal activity at 2 mM Mg2+ in presence of 1 mM ATP | Cyclotella cryptica | |
| Mn2+ | 20% of activation relative to Mg2+, at 2 mM | Cyclotella cryptica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 185000 | - |
4 * 185000, SDS-PAGE | Cyclotella cryptica |
| 740000 | - |
gel filtration | Cyclotella cryptica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cyclotella cryptica | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Cyclotella cryptica |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 14.6 | - |
- |
Cyclotella cryptica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + acetyl-CoA + HCO3- | - |
Cyclotella cryptica | ADP + phosphate + malonyl-CoA | - |
? | |
| ATP + acetyl-CoA + hydrogencarbonate | ATP in form of MgATP2- | Cyclotella cryptica | ADP + phosphate + malonyl-CoA | - |
? | |
| ATP + propionyl-CoA + HCO3- | 10% of the activity relative to acetyl-CoA | Cyclotella cryptica | ADP + phosphate + methylmalonyl-CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 185000, SDS-PAGE | Cyclotella cryptica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.2 | - |
- |
Cyclotella cryptica |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
4°C, 96% loss of activiyt after 48 h | Cyclotella cryptica |
| 6.5 | - |
maximal stability. 4°C, 3% loss of activity after 48 h | Cyclotella cryptica |
| 7.5 | - |
4°C, 85% loss of activity after 48 h | Cyclotella cryptica |
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