Any feedback?
Literature summary for 6.4.1.2 extracted from
- Acetyl-coenzyme A carboxylase from avocado (Persea americana) plastids and spinach (Spinacia oleracea) chloroplasts (1980), Biochem. J., 187, 667-676.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| citrate | activates | Persea americana |  |
| citrate | maximal activation at 3.0 mM, 2fold | Persea americana | |
| isocitrate | maximal activation at 3 mM | Spinacia oleracea | |
| isocitrate | maximal activation at 3 mM | Persea americana | |
General Stability
| General Stability | Organism |
|---|---|
| bovine serum albumin, HCO3-, citrate, and glycerol required for stabilization | Persea americana |
| requires bovine serum albumin, HCO3-, citrate, and glycerol for stabilization | Spinacia oleracea |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| citrate | above 4.0 mM | Persea americana | |
| CuSO4 | - |
Persea americana | |
| diisopropyl phosphorofluoridate | - |
Persea americana | |
| iodoacetamide | - |
Persea americana | |
| Phenyl chloromercuribenzoate | - |
Persea americana | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | effect of citrate or isocitrate on Km-values | Spinacia oleracea | |
| additional information | - |
additional information | effect of citrate or isocitrate on Km-values | Persea americana |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Spinacia oleracea | 9507 | - |
| chloroplast | lamellae | Persea americana | 9507 | - |
| plastid | - |
Persea americana | 9536 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | Mg2+ or Mn2+ essential for activity | Spinacia oleracea | |
| Mg2+ | Mg2+ or Mn2+ essential for activity | Persea americana | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 12000 | - |
x * 12000 + x * 47000 + x * 57000 + x * 72000, SDS-PAGE + 1% mercaptoethanol | Persea americana |
| 47000 | - |
x * 12000 + x * 47000 + x * 57000 + x * 72000, SDS-PAGE + 1% mercaptoethanol | Persea americana |
| 57000 | - |
x * 12000 + x * 47000 + x * 57000 + x * 72000, SDS-PAGE + 1% mercaptoethanol | Persea americana |
| 72000 | - |
x * 12000 + x * 47000 + x * 57000 + x * 72000, SDS-PAGE + 1% mercaptoethanol | Persea americana |
| 650000 | - |
gel filtration | Persea americana |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Persea americana | - |
- |
- |
| Spinacia oleracea | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mesocarp | - |
Persea americana | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 0.1 M potassium phosphate, 0.5 mM EDTA, pH 7.3, 1 mM DTT, 10 mM trisodium citrate, 15 mM NaHCO3, 0.1% bovine serum albumin, 15% v/v glycerol, stable for 2 months | Persea americana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + acetyl-CoA + HCO3- | - |
Spinacia oleracea | ADP + phosphate + malonyl-CoA | - |
? | |
| ATP + acetyl-CoA + HCO3- | - |
Persea americana | ADP + phosphate + malonyl-CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 12000 + x * 47000 + x * 57000 + x * 72000, SDS-PAGE + 1% mercaptoethanol | Persea americana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Persea americana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Persea americana |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
