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Literature summary for 6.4.1.2 extracted from
- Acetyl coenzyme A carboxylase from rat liver (1975), Methods Enzymol., 35B, 3-11.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| citrate | activates | Rattus norvegicus |  |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
General Stability
| General Stability | Organism |
|---|---|
| loss of activity after several freeze/thaw-cycles | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| long-chain acyl-CoA thioesters | - |
Rattus norvegicus | |
| malonyl-CoA | - |
Rattus norvegicus | |
| oleoyl-CoA | - |
Rattus norvegicus | |
| palmitoyl-CoA | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Rattus norvegicus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| phosphate | contains 9.83 mM of phosphate per mg of protein | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 110000 | - |
x * 110000, ultracentrifugation in presence of 6 M guanidine hydrochloride and 0.1 M 2-mercaptoethanol | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 14.6 | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 5°C, under N2, 20 mM sodium citrate, 10 mM potassium phosphate buffer, pH 7.5, 1 mM EDTA, 1 mM DTT, protein concentration greater than 2 mg/ml, stable for 1 week | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + acetyl-CoA + HCO3- | - |
Rattus norvegicus | ADP + phosphate + malonyl-CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 110000, ultracentrifugation in presence of 6 M guanidine hydrochloride and 0.1 M 2-mercaptoethanol | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.65 | - |
- |
Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | 8 | about 90% of maximal activity at pH 7.4 and 8.0 | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| biotin | contains 0.91 mol of biotin per dimer of MW 215000 | Rattus norvegicus | |
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Release 2023.1 (January 2023)
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