Cloned (Comment) | Organism |
---|---|
- |
Pseudomonas aeruginosa |
- |
Methylobacillus flagellatus |
Crystallization (Comment) | Organism |
---|---|
crystal structures of biotin carboxylase domain deletion mutant and of mutant K419A/E421A/E422A reveal an alpha2beta4 stoichiometry | Methylobacillus flagellatus |
Protein Variants | Comment | Organism |
---|---|---|
A465R | mutation in the alpha subunit, abolishes the formation of the holoenzyme, catalytically inactive | Methylobacillus flagellatus |
A55T | mutation in the beta subunit, reduces activity by 50fold and interferes with biotin binding to the active site,mutant exhibits growth on pyruvate similar to the wild type | Pseudomonas aeruginosa |
D502A/E507A | mutation in the alpha subunit, mutant forms a stable holoenzyme, about 40% of wild-type activity | Methylobacillus flagellatus |
H476A/E478A | mutation in the alpha subunit, mutant forms a stable holoenzyme and is fully active | Methylobacillus flagellatus |
H476A/E478A/D502A/E507A | mutation in the alpha subunit, abolishes the formation of the holoenzyme, catalytically inactive | Methylobacillus flagellatus |
K419A/E421A/E422A | mutations bin the beta subunit designed to reduce the surface entropy. The mutations have no effect on the catalytic activity of the enzyme | Methylobacillus flagellatus |
K451stop | mutation in alpha subunit, disrupts holoenzyme formation | Pseudomonas aeruginosa |
K572A | mutation in the beta subunit, abolishes biotinylation and leads to growth defects in pyruvate that are similar to that of a gene deletion mutant | Pseudomonas aeruginosa |
Q452stop | mutation in the alpha subunit, abolishes the formation of the holoenzyme | Methylobacillus flagellatus |
R401E | mutation in the alpha subunit, mutant forms a stable holoenzyme, about 50% of wild-type activity | Methylobacillus flagellatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
340000 | - |
analytical ultracentrifugation | Methylobacillus flagellatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylobacillus flagellatus | Q1H157 | - |
- |
Methylobacillus flagellatus DSM 6875 | Q1H157 | - |
- |
Pseudomonas aeruginosa | - |
- |
- |
Synonyms | Comment | Organism |
---|---|---|
Mfla_1512 | - |
Methylobacillus flagellatus |
General Information | Comment | Organism |
---|---|---|
physiological function | a gene deletion mutant shows similar morphologies to those of the wild-typewhen succinate is supplied as the carbon source. When glucose or pyruvate is provided as the carbon source, the mutation prevents growth. In the colony morphology assay, the wild type initially produces a smooth colony, which forms wrinkle structures in the centre starting on day 3. The deletion mutant and K572A colonies begin to wrinkle on day 2 and exhibit a flatter morphology with several exaggerated wrinkles in a spoke formation | Pseudomonas aeruginosa |