Activating Compound | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Rhizobium etli |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Rhizobium etli |
Protein Variants | Comment | Organism |
---|---|---|
Q552A | the mutation results in loss of the ability to catalyse pyruvate carboxylation, biotin-dependent decarboxylation of oxaloacetate and proton exchange between pyruvate and water | Rhizobium etli |
Q552N | the mutation results in loss of the ability to catalyse pyruvate carboxylation, biotin-dependent decarboxylation of oxaloacetate and proton exchange between pyruvate and water | Rhizobium etli |
R548A | the mutation results in loss of the ability to catalyse pyruvate carboxylation, biotin-dependent decarboxylation of oxaloacetate and proton exchange between pyruvate and water | Rhizobium etli |
R548K | the mutation results in loss of the ability to catalyse pyruvate carboxylation (2% residual activity), biotin-dependent decarboxylation of oxaloacetate and proton exchange between pyruvate and water | Rhizobium etli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
oxamate | - |
Rhizobium etli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Rhizobium etli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
472000 | - |
analytical ultracentrifugation | Rhizobium etli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhizobium etli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HisPur cobalt resin column chromatography | Rhizobium etli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate + HCO3- + H+ | - |
Rhizobium etli | ADP + oxaloacetate + phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
homotetramer | analytical ultracentrifugation | Rhizobium etli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.28 | - |
pyruvate | mutant enzyme R548K, in 100 mM Tris-HCl pH 7.8, 30°C | Rhizobium etli | |
13.9 | - |
pyruvate | wild type enzyme, in 100 mM Tris-HCl pH 7.8, 30°C | Rhizobium etli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Rhizobium etli | |
biotin | - |
Rhizobium etli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
8 | - |
oxamate | mutant enzyme Q552N, in 100 mM Tris-HCl pH 7.8, 30°C | Rhizobium etli | |
10 | - |
oxamate | mutant enzyme R548K, in 100 mM Tris-HCl pH 7.8, 30°C | Rhizobium etli | |
19 | - |
oxamate | wild type enzyme, in 100 mM Tris-HCl pH 7.8, 30°C | Rhizobium etli |