Literature summary for 6.4.1.1 extracted from

Jitrapakdee, S.; Surinya, K.H.; Adina-Zada, A.; Polyak, S.W.; Stojkoski, C.; Smyth, R.; Booker, G.W.; Cleland, W.W.; Attwood, P.V.; Wallace, J.C.
Conserved Glu40 and Glu433 of the biotin carboxylase domain of yeast pyruvate carboxylase I isoenzyme are essential for the association of tetramers (2007), Int. J. Biochem. Cell Biol., 39, 2120-2134.

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Cloned(Commentary)

Cloned (Comment)	Organism
coexpression of pyruvate carboxylase 1 isozyme (Pyc1) with an N-terminal myc tag, together with constructs encoding either the biotin carboxylase domain or the transcarboxylase-biotin carboxyl carrier domain, each with an N-terminal 9-histidine tag	Saccharomyces cerevisiae

Protein Variants

Protein Variants	Comment	Organism
E40R	predominant form of mutant E40R is the monomer. Coexpression of mutant forms with wild type Pyc1 shows that mutations causes severe loss of interaction with wild type Pyc1	Saccharomyces cerevisiae
E433R	predominant form of mutant E433R is the monomer. Coexpression of mutant forms with wild type Pyc1 shows that mutations causes severe loss of interaction with wild type Pyc1	Saccharomyces cerevisiae
R36E	the R36E is much more susceptible to tetramer dissociation and inactivation than the wild type enzyme. Coexpression of mutant forms with wild type Pyc1 shows that the R36E mutation had no effect on the interaction of these subunits with those of wild type Pyc1	Saccharomyces cerevisiae
R36E/E433R	predominant form of mutant R36E/E433R is the monomer. Coexpression of mutant forms with wild type Pyc1 shows that mutations causes severe loss of interaction with wild type Pyc1	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	P11154	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Saccharomyces cerevisiae

Subunits

Subunits	Comment	Organism
tetramer	Glu40 and Glu433 play essential roles in subunit interactions	Saccharomyces cerevisiae

Synonyms

Synonyms	Comment	Organism
Pyc1	-	Saccharomyces cerevisiae
pyruvate carboxylase 1	-	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)