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Literature summary for 6.4.1.1 extracted from
- Cloning and characterization of the pyruvate carboxylase from Sinorhizobium meliloti Rm1021 (2001), Arch. Microbiol., 176, 355-363.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| acetyl-CoA | activates, half-maximal activity at 0.23 mM | Sinorhizobium meliloti |  |
| propionyl-CoA | activates less efficiently than acetyl-CoA | Sinorhizobium meliloti | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Sinorhizobium meliloti |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-oxoglutarate | less than 10% inhibition at 5 mM | Sinorhizobium meliloti | |
| ADP | 27% inhibition at 5 mM | Sinorhizobium meliloti | |
| L-aspartate | 6.4% inhibition at 10 mM | Sinorhizobium meliloti | |
| L-glutamate | less than 10% inhibition at 5 mM | Sinorhizobium meliloti | |
| NaCl | slight inhibition at 20 mM | Sinorhizobium meliloti | |
| oxaloacetate | inhibition below 1 mM | Sinorhizobium meliloti | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.26 | - |
pyruvate | pH 8 | Sinorhizobium meliloti | |
| 0.66 | - |
ATP | pH 8 | Sinorhizobium meliloti | |
| 5.6 | - |
HCO3- | pH 8 | Sinorhizobium meliloti | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | 1.6fold stimulation at 20 mM | Sinorhizobium meliloti | |
| MgCl2 | 7.5 mM | Sinorhizobium meliloti | |
| MnCl2 | 7.5 mM, can partially replace MgCl2 | Sinorhizobium meliloti | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 120000 | - |
4 * 120000, SDS-PAGE | Sinorhizobium meliloti |
| 500000 | - |
gel filtration | Sinorhizobium meliloti |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + pyruvate + HCO3- | Sinorhizobium meliloti | - |
ADP + phosphate + oxaloacetate | - |
? | |
| ATP + pyruvate + HCO3- | Sinorhizobium meliloti Rm1021 | - |
ADP + phosphate + oxaloacetate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sinorhizobium meliloti | - |
Rm1021 | - |
| Sinorhizobium meliloti Rm1021 | - |
Rm1021 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + pyruvate + HCO3- | - |
Sinorhizobium meliloti | ADP + phosphate + oxaloacetate | - |
? | |
| ATP + pyruvate + HCO3- | - |
Sinorhizobium meliloti Rm1021 | ADP + phosphate + oxaloacetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 120000, SDS-PAGE | Sinorhizobium meliloti |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| biotin | activity is dependent on biotin availability | Sinorhizobium meliloti | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.22 | - |
oxaloacetate | pH 8 | Sinorhizobium meliloti | |
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