Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli | Salmonella enterica subsp. enterica serovar Typhimurium |
Protein Variants | Comment | Organism |
---|---|---|
D45N | less than 0.2% of amidation activity with glutamine and with NH4+, compared to wild-type values | Salmonella enterica subsp. enterica serovar Typhimurium |
D48N | less than 0.3% of amidation activity with glutamine and with NH4+, compared to wild-type values | Salmonella enterica subsp. enterica serovar Typhimurium |
D97N | 32.4% of wild-type amidation activity with glutamine, 16.5% of wild-type amidation activity with NH4+ | Salmonella enterica subsp. enterica serovar Typhimurium |
E90Q | less than 0.02% of amidation activity with glutamine and with NH4+, compared to wild-type values | Salmonella enterica subsp. enterica serovar Typhimurium |
L47A | 10.6% of wild-type amidation activity with glutamine, 44% of wild-type amidation activity with NH4+, mutation specifically decreases the affinity of the enzyme to the cobyrinic acid c-monoamide | Salmonella enterica subsp. enterica serovar Typhimurium |
Y46A | 3.8% of wild-type amidation activity with glutamine, 2% of wild-type amidation activity with NH4+, mutation specifically decreases the affinity of the enzyme to the cobyrinic acid c-monoamide | Salmonella enterica subsp. enterica serovar Typhimurium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0003 | - |
cobyrinic acid c-monoamide | mutant enzyme D97N | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.00041 | - |
cobyrinic acid c-monoamide | wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.00074 | - |
cobyrinic acid | pH 7.7, wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.0027 | - |
cobyrinic acid c-monoamide | mutant enzyme L47A | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.0027 | - |
ATP | pH 7.7, wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.011 | - |
cobyrinic acid c-monoamide | mutant enzyme Y46A | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.053 | - |
glutamine | pH 7.7, wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | |
26.2 | - |
NH4+ | pH 7.7, wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
1 * 50000, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
55000 | - |
gel filtration | Salmonella enterica subsp. enterica serovar Typhimurium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cobyrinic acid + ATP + glutamine | model for catalytic mechanism in which CbiA catalyzes the amidation of the c-carboxylate and then the intermediate is released into solution and binds to the same catalytic site for the amidation of the a-carboxylate | Salmonella enterica subsp. enterica serovar Typhimurium | cobyrinic acid a,c-diamide + ADP + phosphate + glutamate | - |
? | |
cobyrinic acid + ATP + NH4+ | model for catalytic mechanism in which CbiA catalyzes the amidation of the c-carboxylate and then the intermediate is released into solution and binds to the same catalytic site for the amidation of the a-carboxylate | Salmonella enterica subsp. enterica serovar Typhimurium | cobyrinic acid a,c-diamide + ADP + phosphate | - |
? | |
cobyrinic acid c-monoamide + ATP + glutamine | - |
Salmonella enterica subsp. enterica serovar Typhimurium | cobyrinic acid a,c-diamide + ADP + phosphate + glutamate | - |
? | |
cobyrinic acid c-monoamide + ATP + NH4+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | cobyrinic acid a,c-diamide + ADP + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 50000, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
Synonyms | Comment | Organism |
---|---|---|
CbiA | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
cobyrinic acid c-monoamide | mutant enzyme Y46A | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.04 | - |
cobyrinic acid c-monoamide | mutant enzyme D97N | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.057 | - |
cobyrinic acid c-monoamide | mutant enzyme L47A | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.13 | - |
ATP | pH 7.7, wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.16 | - |
glutamine | pH 7.7, wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.16 | - |
cobyrinic acid | pH 7.7, wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.18 | - |
NH4+ | pH 7.7, wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.2 | - |
cobyrinic acid c-monoamide | wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | 8 | - |
Salmonella enterica subsp. enterica serovar Typhimurium |