Any feedback?
Literature summary for 6.3.5.7 extracted from
- The archaeal transamidosome for RNA-dependent glutamine biosynthesis (2010), Nucleic Acids Res., 38, 5774-5783.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli as a His-tagged fusion protein | Methanothermobacter thermautotrophicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0017 | - |
Glu-tRNAGln | 37°C, pH not specified in the publication | Methanothermobacter thermautotrophicus |  |
| 0.0023 | - |
Glu-tRNAGln | 37°C, pH not specified in the publication, in the presence of glutamyl-tRNA synthetase (0.002 mM) | Methanothermobacter thermautotrophicus | |
| 0.0024 | - |
Glu-tRNAGln | 37°C, pH not specified in the publication, in the presence of bovine serum albumin (0.002 mM) | Methanothermobacter thermautotrophicus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanothermobacter thermautotrophicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using Ni-NTA chromatography | Methanothermobacter thermautotrophicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + Glu-tRNAGln + L-glutamine | - |
Methanothermobacter thermautotrophicus | ADP + phosphate + Gln-tRNAGln + L-glutamate | - |
? | |
| additional information | the association of archaeal glutamyl-tRNA synthetase (ND-GluRS) with GatDE sequesters the tRNA synthetase for Gln-tRNAGln formation, with GatDE reducing the affinity of glutamyl-tRNA synthetase (ND-GluRS) for tRNAGlu 13fold | Methanothermobacter thermautotrophicus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | gel filtration, GatDE forms a homodimer with the binding interface between the D-subunits | Methanothermobacter thermautotrophicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GatDE | - |
Methanothermobacter thermautotrophicus |
| Glu-amidotransferase | - |
Methanothermobacter thermautotrophicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Methanothermobacter thermautotrophicus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.9 | - |
Glu-tRNAGln | 37°C, pH not specified in the publication | Methanothermobacter thermautotrophicus | |
| 0.9 | - |
Glu-tRNAGln | 37°C, pH not specified in the publication, in the presence of bovine serum albumin (0.002 mM) | Methanothermobacter thermautotrophicus | |
| 1.2 | - |
Glu-tRNAGln | 37°C, pH not specified in the publication, in the presence of glutamyl-tRNA synthetase (0.002 mM) | Methanothermobacter thermautotrophicus | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 400 | - |
Glu-tRNAGln | 37°C, pH not specified in the publication, in the presence of bovine serum albumin (0.002 mM) | Methanothermobacter thermautotrophicus | |
| 500 | - |
Glu-tRNAGln | 37°C, pH not specified in the publication | Methanothermobacter thermautotrophicus | |
| 600 | - |
Glu-tRNAGln | 37°C, pH not specified in the publication, in the presence of glutamyl-tRNA synthetase (0.002 mM) | Methanothermobacter thermautotrophicus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
