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Literature summary for 6.3.5.6 extracted from

  • Fischer, F.; Huot, J.L.; Lorber, B.; Diss, G.; Hendrickson, T.L.; Becker, H.D.; Lapointe, J.; Kern, D.
    The asparagine-transamidosome from Helicobacter pylori: a dual-kinetic mode in non-discriminating aspartyl-tRNA synthetase safeguards the genetic code (2012), Nucleic Acids Res., 40, 4965-4976.
    View publication on PubMedView publication on EuropePMC
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0021
-
 Asp-tRNAAsn in 100 mM Na-HEPES buffer pH 7.2, 30 mM KCl, 12 mM MgCl2, at 37°C Helicobacter pylori

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + Asp-tRNAAsn + L-glutamine + H2O Helicobacter pylori
-
 ADP + phosphate + Asn-tRNAAsn + L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Helicobacter pylori
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-cellulose column chromatography and Ni-NTA column chromatography Helicobacter pylori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + Asp-tRNAAsn + L-glutamine + H2O
-
 Helicobacter pylori ADP + phosphate + Asn-tRNAAsn + L-glutamate
-
 ?

Synonyms

Synonyms Comment Organism
GatCAB
-
 Helicobacter pylori

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.25
-
 Asp-tRNAAsn in 100 mM Na-HEPES buffer pH 7.2, 30 mM KCl, 12 mM MgCl2, at 37°C Helicobacter pylori

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Helicobacter pylori