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Literature summary for 6.3.5.6 extracted from

  • Sheppard, K.; Akochy, P.M.; Salazar, J.C.; Soell, D.
    The Helicobacter pylori amidotransferase GatCAB is equally efficient in glutamine-dependent transamidation of Asp-tRNAAsn and Glu-tRNAGln (2007), J. Biol. Chem., 282, 11866-11873.
    View publication on PubMed
Search for more literature for 6.3.5.6

Protein Variants

Protein Variants Comment Organism
S128T mutant protein retains significant glutaminase activity and transamidase activity in the presence of Gln Helicobacter pylori
S152A mutant is glutaminase inactive Helicobacter pylori
S152T mutant is glutaminase inactive Helicobacter pylori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00095
-
 Asp-tRNAAsn 37°C, pH 7.2, amidotransferase activity Helicobacter pylori
0.00118
-
 Asp-tRNAGln 37°C, pH 7.2, amidotransferase activity Helicobacter pylori
0.0207
-
 Gln 37°C, pH 7.2, amidotransferase activity Helicobacter pylori
0.0224
-
 L-aspartyl-tRNAAsn 37°C, pH 7.2, amidotransferase activity Helicobacter pylori
0.0402
-
 Gln 37°C, cosubstrate Asp-tRNAAsn + ATP, glutaminase activity Helicobacter pylori
0.0509
-
 Gln 37°C, cosubstrate Glu-tRNAGln + ATP, glutaminase activity Helicobacter pylori
0.2068
-
 ATP 37°C, pH 7.2, amidotransferase activity Helicobacter pylori

Organism

Organism UniProt Comment Textmining
Helicobacter pylori
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Helicobacter pylori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + Asp-tRNAAsn + L-glutamine the enzyme transamidates Asp-tRNAAsn and Glu-tRNAGln with similar efficiency Helicobacter pylori ADP + phosphate + Asn-tRNAAsn + L-glutamate
-
 ?
ATP + Glu-tRNAGln + L-glutamine the enzyme transamidates Asp-tRNAAsn and Glu-tRNAGln with similar efficiency. GatCAB uses the amide donor glutamine 129fold more efficiently than asparagine Helicobacter pylori ADP + phosphate + Gln-tRNAGln + L-glutamate
-
 ?
ATP + L-aspartyl-tRNAAsn + L-glutamine + H2O
-
 Helicobacter pylori ADP + phosphate + L-asparaginyl-tRNAAsn + L-glutamate
-
 ?

Synonyms

Synonyms Comment Organism
GatCAB
-
 Helicobacter pylori

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.027
-
 L-aspartyl-tRNAAsn 37°C, pH 7.2, amidotransferase activity Helicobacter pylori
0.052 2.1 Gln 37°C, pH 7.2, cosubstrate: Glu-tRNAGln Helicobacter pylori
1.3
-
 Asp-tRNAAsn 37°C, pH 7.2, amidotransferase activity Helicobacter pylori
3 6 Gln 37°C, pH 7.2, amidotransferase activity Helicobacter pylori
3.49
-
 Gln 37°C, pH 7.2, amidotransferase activity Helicobacter pylori
3.61
-
 Asp-tRNAGln 37°C, pH 7.2, amidotransferase activity Helicobacter pylori
6.1
-
 ATP 37°C, pH 7.2, amidotransferase activity Helicobacter pylori
10.29
-
 Gln 37°C, pH 7.2, cosubstrate: Glu-tRNAGln Helicobacter pylori
11.8
-
 Gln 37°C, pH 7.2, cosubstrate Asp-tRNAAsn + ATP, glutaminase activity Helicobacter pylori