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Literature summary for 6.3.5.5 extracted from

  • McPhail, D.; Cheung, M.K.; Brown, J.; Shepherdson, M.
    Factors accelerating pyrimidine production in Deinococcus radiophilus (2009), Arch. Microbiol., 191, 73-82.
    View publication on PubMed
Search for more literature for 6.3.5.5

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Deinococcus radiophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-glutamine + HCO3- + H2O Deinococcus radiophilus
-
 ADP + phosphate + L-glutamate + carbamoyl phosphate
-
 ?
ATP + L-glutamine + HCO3- + H2O Deinococcus radiophilus NCIMB 10648
-
 ADP + phosphate + L-glutamate + carbamoyl phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Deinococcus radiophilus
-
 NCIMB 10648
-
Deinococcus radiophilus NCIMB 10648
-
 NCIMB 10648
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme partially Deinococcus radiophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-glutamine + HCO3- + H2O
-
 Deinococcus radiophilus ADP + phosphate + L-glutamate + carbamoyl phosphate
-
 ?
ATP + L-glutamine + HCO3- + H2O substrate-channelling, the enzyme is also active with NH3 as nitrogen source, cf. EC 6.3.4.16 Deinococcus radiophilus ADP + phosphate + L-glutamate + carbamoyl phosphate
-
 ?
ATP + L-glutamine + HCO3- + H2O
-
 Deinococcus radiophilus NCIMB 10648 ADP + phosphate + L-glutamate + carbamoyl phosphate
-
 ?
ATP + L-glutamine + HCO3- + H2O substrate-channelling, the enzyme is also active with NH3 as nitrogen source, cf. EC 6.3.4.16 Deinococcus radiophilus NCIMB 10648 ADP + phosphate + L-glutamate + carbamoyl phosphate
-
 ?
additional information assay method development based on the fixation of 14C from the substrate bicarbonate to give radioactive products. In the coupled assay the carbamoyl phosphate product of the enzyme is converted to carbamoyl aspartate in the presence of aspartate and aspartate transcarbamoylase, substrate-channelling between aspartate aminotransferase and CPSase, overview Deinococcus radiophilus ?
-
 ?
additional information assay method development based on the fixation of 14C from the substrate bicarbonate to give radioactive products. In the coupled assay the carbamoyl phosphate product of the enzyme is converted to carbamoyl aspartate in the presence of aspartate and aspartate transcarbamoylase, substrate-channelling between aspartate aminotransferase and CPSase, overview Deinococcus radiophilus NCIMB 10648 ?
-
 ?

Synonyms

Synonyms Comment Organism
carbamoyl phosphate synthetase
-
 Deinococcus radiophilus
CPSase
-
 Deinococcus radiophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Deinococcus radiophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Deinococcus radiophilus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Deinococcus radiophilus