Literature summary for 6.3.5.5 extracted from

Kothe, M.; Purcarea, C.; Guy, H.I.; Evans, D.R.; Powers-Lee, S.G.
Direct demonstration of carbamoyl phosphate formation on the C-terminal domain of carbamoyl phosphate synthetase (2005), Protein Sci., 14, 37-44.

Search for more literature for 6.3.5.5

Cloned(Commentary)

Cloned (Comment)	Organism
subcloning of each of the subunits into the pET-28b vector, expression in Escherichia coli	Methanocaldococcus jannaschii

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
56000	-	x * 56000 (subunit N) + x * 71000 (subunit C), SDS-PAGE	Methanocaldococcus jannaschii
71000	-	x * 56000 (subunit N) + x * 71000 (subunit C), SDS-PAGE	Methanocaldococcus jannaschii

Organism

Organism	UniProt	Comment	Textmining
Methanocaldococcus jannaschii	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2 ATP + L-Gln + HCO3-	cross-talk between domain N and C is required for product release from subunit C	Methanocaldococcus jannaschii	2 ADP + phosphate + L-Glu + carbamoyl phosphate	-	?

Subunits

Subunits	Comment	Organism
?	x * 56000 (subunit N) + x * 71000 (subunit C), SDS-PAGE	Methanocaldococcus jannaschii

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Release 2023.1 (January 2023)