Protein Variants | Comment | Organism |
---|---|---|
A126M | mutation in large subunit significantly decreases synthesis of carbamoyl phosphate without completely inactivating the enzyme | Escherichia coli |
N301K | mutation in large subunit significantly decreases synthesis of carbamoyl phosphate without completely inactivating the enzyme | Escherichia coli |
P360L | mutation in large subunit significantly decreases synthesis of carbamoyl phosphate without completely inactivating the enzyme | Escherichia coli |
Q262P | mutation causes marked enzyme instability, mutation in large subunit significantly decreases synthesis of carbamoyl phosphate without completely inactivating the enzyme | Escherichia coli |
R169H | mutation in large subunit significantly decreases synthesis of carbamoyl phosphate without completely inactivating the enzyme | Escherichia coli |
R675L | mutation in large subunit significantly decreases synthesis of carbamoyl phosphate without completely inactivating the enzyme | Escherichia coli |
S789P | mutation in large subunit significantly decreases synthesis of carbamoyl phosphate without completely inactivating the enzyme | Escherichia coli |
V640R | mutation in large subunit significantly decreases synthesis of carbamoyl phosphate without completely inactivating the enzyme | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
ATP | pH 8.0, 37°C, wild-type enzyme | Escherichia coli | |
0.32 | - |
ATP | pH 8.0, 25°C, wild-type enzyme | Escherichia coli | |
0.45 | - |
ATP | pH 8.0, 25°C, mutant enzyme Q262P | Escherichia coli | |
0.64 | - |
HCO3- | pH 8.0, 37°C, mutant enzyme P360L | Escherichia coli | |
0.76 | - |
HCO3- | pH 8.0, 25°C, mutant enzyme Q262P | Escherichia coli | |
0.76 | - |
ATP | pH 8.0, 37°C, mutant enzyme R675L | Escherichia coli | |
0.87 | - |
ATP | pH 8.0, 37°C, mutant enzyme A126M | Escherichia coli | |
1.14 | - |
ATP | pH 8.0, 37°C, mutant enzyme R169H | Escherichia coli | |
1.33 | - |
HCO3- | pH 8.0, 25°C, wild-type enzyme | Escherichia coli | |
1.9 | - |
HCO3- | pH 8.0, 37°C, wild-type enzyme | Escherichia coli | |
3.02 | - |
ATP | pH 8.0, 37°C, mutant enzyme V640R | Escherichia coli | |
3.65 | - |
HCO3- | pH 8.0, 37°C, mutant enzyme V640R | Escherichia coli | |
4.46 | - |
HCO3- | pH 8.0, 37°C, mutant enzyme R675L | Escherichia coli | |
8.13 | - |
ATP | pH 8.0, 37°C, mutant enzyme S789P | Escherichia coli | |
8.36 | - |
ATP | pH 8.0, 37°C, mutant enzyme P360L | Escherichia coli | |
12.6 | - |
HCO3- | pH 8.0, 37°C, mutant enzyme R169H | Escherichia coli | |
23.8 | - |
HCO3- | pH 8.0, 37°C, mutant enzyme S789P | Escherichia coli | |
32.5 | - |
HCO3- | pH 8.0, 37°C, mutant enzyme A126M | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ATP + L-Gln + HCO3- | - |
Escherichia coli | 2 ADP + phosphate + L-Glu + carbamoyl phosphate | - |
? |