Literature summary for 6.3.5.4 extracted from

Xu, H.; Curtis, T.; Powers, S.; Raffan, S.; Gao, R.; Huang, J.; Heiner, M.; Gilbert, D.; Halford, N.
Genomic, biochemical, and modeling analyses of asparagine synthetases from wheat (2018), Front. Plant Sci., 8, 2237 .

Search for more literature for 6.3.5.4

Cloned(Commentary)

Cloned (Comment)	Organism
gene ASN1, a single copy gene located on chromosome 5 of each genome, genetic organization, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in Escherichia coli strain Rosetta DE3	Triticum aestivum
gene ASN2, a single copy gene located on chromosome 3 of each genome, variety Chinese Spring lacks a TaASN2 gene in the B genome, DNA and amino acid sequence determination and analysis, sequence comparisons, genetic organization, recombinant expression in Escherichia coli strain Rosetta DE3	Triticum aestivum
gene ASN3, two copies of TaASN3 are found on chromosome 1 of each genome, termed TaASN3.1 and TaASN3.2, DNA and amino acid sequence determination and analysis, sequence comparisons, genetic organization, recombinant expression in Escherichia coli strain Rosetta DE3	Triticum aestivum
gene ASN4, a single copy gene located on chromosome 4 of each genome, DNA and amino acid sequence determination and analysis, sequence comparisons	Triticum aestivum

Protein Variants

Protein Variants	Comment	Organism
additional information	variety Chinese Spring lacks a TaASN2 gene in the B genome	Triticum aestivum

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Triticum aestivum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + L-aspartate + L-glutamine + H2O	Triticum aestivum	-	AMP + diphosphate + L-asparagine + L-glutamate	-	?

Organism

Organism	UniProt	Comment	Textmining
Triticum aestivum	-	cv. Spark	-
Triticum aestivum	A0A1D5VHV8	cv. Spark	-
Triticum aestivum	A0A1D5YL35	cv. Spark	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	-	Triticum aestivum	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-aspartate + L-glutamine + H2O	-	Triticum aestivum	AMP + diphosphate + L-asparagine + L-glutamate	-	?
additional information	the enzyme continues to produce glutamate even when the synthesis of asparagine has ceased due to a lack of aspartate	Triticum aestivum	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 65060, ASN1, sequence calculation	Triticum aestivum
?	x * 65490, ASN1, sequence calculation	Triticum aestivum
?	x * 66240, ASN1, sequence calculation	Triticum aestivum

Synonyms

Synonyms	Comment	Organism
Asparagine synthetase	-	Triticum aestivum
TaASN1	-	Triticum aestivum
TaASN2	-	Triticum aestivum
TaASN4	-	Triticum aestivum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Triticum aestivum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.6	-	assay at	Triticum aestivum

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Triticum aestivum

General Information

General Information	Comment	Organism
physiological function	asparagine synthesis is catalyzed by the enzyme asparagine synthetase, and occurs by the ATP-dependent transfer of the amino group of glutamine to a molecule of aspartate to generate glutamate and asparagine	Triticum aestivum
physiological function	asparagine synthesis is catalyzed by the enzyme asparagine synthetase, and occurs by the ATP-dependent transfer of the amino group of glutamine to a molecule of aspartate to generate glutamate and asparagine, isozyme TaASN1 is an active asparagine synthetase, producing asparagine and glutamate from glutamine and aspartate, reaction modeling. There are large differences in the free asparagine concentration of grain from different wheat varieties	Triticum aestivum
physiological function	asparagine synthesis is catalyzed by the enzyme asparagine synthetase, and occurs by the ATP-dependent transfer of the amino group of glutamine to a molecule of aspartate to generate glutamate and asparagine, isozyme TaASN2 is an active asparagine synthetase, producing asparagine and glutamate from glutamine and aspartate, reaction modeling, There are also large differences in the free asparagine concentration of grain from different wheat varieties	Triticum aestivum

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Release 2023.1 (January 2023)