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Literature summary for 6.3.5.4 extracted from

  • Canales, J.; Rueda-Lopez, M.; Craven-Bartle, B.; Avila, C.; Canovas, F.M.
    Novel insights into regulation of asparagine synthetase in conifers (2012), Front. Plant Sci., 3, 100.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene PpAS1, DNA and amino acid sequence analysis, sequence comparisons, phylogenetic analysis, genetic regulation of AS1 transcription, overview Pinus pinaster
gene PpAS2, DNA and amino acid sequence analysis, sequence comparisons, phylogenetic analysis Pinus pinaster

Protein Variants

Protein Variants Comment Organism
additional information PpAS2 transcript abundance is not affected by any nitrogen treatments or by water stress, PpAS2 expression is essentially constitutive Pinus pinaster
up gene PpAS2 expression is upregulated during drought, but the level of PpAS2 transcripts is not altered by darkness Pinus pinaster

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66500
-
x * 66500, about, sequence calculation Pinus pinaster
66800
-
x * 66800, about, sequence calculation Pinus pinaster

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-aspartate + L-glutamine + H2O Pinus pinaster
-
AMP + diphosphate + L-asparagine + L-glutamate
-
?

Organism

Organism UniProt Comment Textmining
Pinus pinaster D9IXC8 AS2; gene PpAS2
-
Pinus pinaster E7EAQ2 AS1; gene PpAS1
-

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon
-
Pinus pinaster
-
hypocotyl
-
Pinus pinaster
-
additional information comparative expression analysis of isozymes during pine development, quantitative real time-PCR expression analysis, overview. PpAS2 expression is essentially constitutive Pinus pinaster
-
additional information comparative expressionanalysis of isozymes during pine development, quantitative real time-PCR expression analysis, overview Pinus pinaster
-
root
-
Pinus pinaster
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-aspartate + L-glutamine + H2O
-
Pinus pinaster AMP + diphosphate + L-asparagine + L-glutamate
-
?

Subunits

Subunits Comment Organism
? x * 66500, about, sequence calculation Pinus pinaster
? x * 66800, about, sequence calculation Pinus pinaster

Synonyms

Synonyms Comment Organism
Asparagine synthetase
-
Pinus pinaster

Cofactor

Cofactor Comment Organism Structure
ATP
-
Pinus pinaster

Expression

Organism Comment Expression
Pinus pinaster gene PpAS1 expression is upregulated during drought, and in the dark in all organs. PpAS1 transcripts accumulate with seedlings that are grown under high concentrations of nitrate or ammonium. Ability of PtMYB1 to activate the transcription of PpAS1 in vivo, PtMYB1 negatively regulates PpAS1 promoter in pine protoplasts up

General Information

General Information Comment Organism
evolution genes PpAS1 and PpAS2 encode both class II asparagine synthetases suggesting an ancient origin of the genes in plants. Amino acid residues essential for aspartate, AMP, and glutamine binding are conserved Pinus pinaster
evolution genes PpAS1 and PpAS2 encode both class II asparagine synthetases suggesting an ancient origin of the genes in plants. Amino acid residues essential for aspartate, AMP, and glutamine binding are conserved, except valine268, included in the AMP binding site, which has been replaced by an isoleucine in the AS2 sequence Pinus pinaster
physiological function the expression of PpAS1 is regulated by developmental and environmental factors Pinus pinaster