Cloned (Comment) | Organism |
---|---|
gene PpAS1, DNA and amino acid sequence analysis, sequence comparisons, phylogenetic analysis, genetic regulation of AS1 transcription, overview | Pinus pinaster |
gene PpAS2, DNA and amino acid sequence analysis, sequence comparisons, phylogenetic analysis | Pinus pinaster |
Protein Variants | Comment | Organism |
---|---|---|
additional information | PpAS2 transcript abundance is not affected by any nitrogen treatments or by water stress, PpAS2 expression is essentially constitutive | Pinus pinaster |
up | gene PpAS2 expression is upregulated during drought, but the level of PpAS2 transcripts is not altered by darkness | Pinus pinaster |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66500 | - |
x * 66500, about, sequence calculation | Pinus pinaster |
66800 | - |
x * 66800, about, sequence calculation | Pinus pinaster |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate + L-glutamine + H2O | Pinus pinaster | - |
AMP + diphosphate + L-asparagine + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pinus pinaster | D9IXC8 | AS2; gene PpAS2 | - |
Pinus pinaster | E7EAQ2 | AS1; gene PpAS1 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cotyledon | - |
Pinus pinaster | - |
hypocotyl | - |
Pinus pinaster | - |
additional information | comparative expression analysis of isozymes during pine development, quantitative real time-PCR expression analysis, overview. PpAS2 expression is essentially constitutive | Pinus pinaster | - |
additional information | comparative expressionanalysis of isozymes during pine development, quantitative real time-PCR expression analysis, overview | Pinus pinaster | - |
root | - |
Pinus pinaster | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate + L-glutamine + H2O | - |
Pinus pinaster | AMP + diphosphate + L-asparagine + L-glutamate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 66500, about, sequence calculation | Pinus pinaster |
? | x * 66800, about, sequence calculation | Pinus pinaster |
Synonyms | Comment | Organism |
---|---|---|
Asparagine synthetase | - |
Pinus pinaster |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Pinus pinaster |
Organism | Comment | Expression |
---|---|---|
Pinus pinaster | gene PpAS1 expression is upregulated during drought, and in the dark in all organs. PpAS1 transcripts accumulate with seedlings that are grown under high concentrations of nitrate or ammonium. Ability of PtMYB1 to activate the transcription of PpAS1 in vivo, PtMYB1 negatively regulates PpAS1 promoter in pine protoplasts | up |
General Information | Comment | Organism |
---|---|---|
evolution | genes PpAS1 and PpAS2 encode both class II asparagine synthetases suggesting an ancient origin of the genes in plants. Amino acid residues essential for aspartate, AMP, and glutamine binding are conserved | Pinus pinaster |
evolution | genes PpAS1 and PpAS2 encode both class II asparagine synthetases suggesting an ancient origin of the genes in plants. Amino acid residues essential for aspartate, AMP, and glutamine binding are conserved, except valine268, included in the AMP binding site, which has been replaced by an isoleucine in the AS2 sequence | Pinus pinaster |
physiological function | the expression of PpAS1 is regulated by developmental and environmental factors | Pinus pinaster |