Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Vibrio cholerae serotype O1 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha,beta-methylene-ATP | - |
Vibrio cholerae serotype O1 | |
Asn | - |
Vibrio cholerae serotype O1 | |
diphosphate | - |
Vibrio cholerae serotype O1 | |
L-cysteinesulfinic acid | - |
Vibrio cholerae serotype O1 | |
L-glutamic acid gamma-methyl ester | - |
Vibrio cholerae serotype O1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio cholerae serotype O1 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate + L-glutamine | the reaction sequence begins with the ordered addition of ATP and aspartate. Diphosphate is released, followed by the addition of ammonia and the release of asparagine and AMP. Glutamine is simultaneously hydrolyzed at a second site and the ammonia intermediate diffuses through an interdomain protein tunnel from the site of production to the site of utilization. The data are also consistent with the dead-end binding of asparagine to the glutamine binding site and diphosphate with free enzyme. The rate of hydrolysis of glutamine is largely independent of the activation of aspartate and thus the reaction rates at the two active sites are essentially uncoupled from one another | Vibrio cholerae serotype O1 | AMP + diphosphate + L-asparagine + L-glutamate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AsnB | - |
Vibrio cholerae serotype O1 |
Asparagine synthetase B | - |
Vibrio cholerae serotype O1 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.8 | - |
ATP | - |
Vibrio cholerae serotype O1 | |
5.8 | - |
L-asparagine | - |
Vibrio cholerae serotype O1 | |
5.8 | - |
glutamine | - |
Vibrio cholerae serotype O1 |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.021 | - |
diphosphate | Kis, versus ATP | Vibrio cholerae serotype O1 | |
0.023 | - |
Asn | Kis, versus Gln | Vibrio cholerae serotype O1 | |
0.65 | - |
diphosphate | Kii, versus Asp | Vibrio cholerae serotype O1 | |
0.85 | - |
diphosphate | Kii, versus NH3 or versus Gln | Vibrio cholerae serotype O1 | |
0.85 | - |
diphosphate | Kis, versus Asp | Vibrio cholerae serotype O1 | |
1.1 | - |
L-cysteine sulfinic acid | Kis, versus Asp | Vibrio cholerae serotype O1 | |
1.1 | - |
alpha,beta-methylene-ATP | Kis, versus ATP | Vibrio cholerae serotype O1 | |
2.2 | - |
L-cysteine sulfinic acid | Kii, versus NH3 | Vibrio cholerae serotype O1 | |
2.5 | - |
L-cysteine sulfinic acid | Kii, versus ATP | Vibrio cholerae serotype O1 | |
2.6 | - |
alpha,beta-methylene-ATP | Kis or Kii, versus Asp | Vibrio cholerae serotype O1 | |
3.2 | - |
alpha,beta-methylene-ATP | Kii, versus Gln | Vibrio cholerae serotype O1 | |
3.7 | - |
alpha,beta-methylene-ATP | Kii, versus NH3 | Vibrio cholerae serotype O1 | |
6.4 | - |
L-cysteine sulfinic acid | Kii, versus Gln | Vibrio cholerae serotype O1 | |
6.5 | - |
L-glutamic acid gamma-methyl ester | Kis, versus NH3 | Vibrio cholerae serotype O1 | |
7.2 | - |
L-cysteine sulfinic acid | Kis, versus Gln | Vibrio cholerae serotype O1 | |
8.9 | - |
L-glutamic acid gamma-methyl ester | Kis, versus Gln | Vibrio cholerae serotype O1 | |
27 | - |
L-glutamic acid gamma-methyl ester | Kii, versus Asp | Vibrio cholerae serotype O1 | |
29 | - |
L-glutamic acid gamma-methyl ester | Kii, versus ATP | Vibrio cholerae serotype O1 |