Cloned (Comment) | Organism |
---|---|
C-terminally tagged enzyme, baculovirus-based expression system, the recombinant enzyme is correctly processed, exhibits high activity and is stable on prolonged storage at -80°C | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
ATP | pH 8, reaction with glutamine, C-terminally tagged recombinant enzyme | Homo sapiens | |
0.11 | - |
ATP | pH 8, reaction with NH3, C-terminally tagged recombinant enzyme | Homo sapiens | |
0.38 | - |
aspartic acid | pH 8, reaction with glutamine, C-terminally tagged recombinant enzyme | Homo sapiens | |
1.3 | - |
L-aspartic acid | pH 8, reaction with NH3, C-terminally tagged recombinant enzyme | Homo sapiens | |
1.7 | - |
NH3 | pH 8, C-terminally tagged recombinant enzyme | Homo sapiens | |
1.9 | - |
L-glutamine | pH 8, C-terminally tagged recombinant enzyme | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
T-cell | - |
Homo sapiens | - |
Storage Stability | Organism |
---|---|
-80°C, recombinant C-terminally tagged enzyme is stable on prolonged storage | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Asp + L-Gln | - |
Homo sapiens | AMP + diphosphate + Asn + Glu | - |
? | |
ATP + L-Asp + NH3 | - |
Homo sapiens | AMP + diphosphate + Asn | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
L-aspartic acid | pH 8, reaction with glutamine, C-terminally tagged recombinant enzyme | Homo sapiens | |
1.6 | - |
ATP | pH 8, reaction with NH3, C-terminally tagged recombinant enzyme | Homo sapiens | |
1.7 | - |
glutamine | pH 8, C-terminally tagged recombinant enzyme | Homo sapiens | |
1.7 | - |
ATP | pH 8, reaction with glutamine, C-terminally tagged recombinant enzyme | Homo sapiens | |
1.7 | - |
L-aspartic acid | pH 8, reaction with NH3, C-terminally tagged recombinant enzyme | Homo sapiens | |
1.8 | - |
NH3 | pH 8, C-terminally tagged recombinant enzyme | Homo sapiens |