Cloned (Comment) | Organism |
---|---|
HvAS1, from leaves, located to the long arm of chromosome 5, sequencing | Hordeum vulgare |
HvAS2, from leaves, located to the short arm of chromosome 3, sequencing | Hordeum vulgare |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
x * 65500, amino acid sequence calculation, x * 65000, Western blot analysis, HvAS1 | Hordeum vulgare |
65200 | - |
x * 65200, HvAS2, amino acid sequence calculation | Hordeum vulgare |
65500 | - |
x * 65500, amino acid sequence calculation, x * 65000, Western blot analysis, HvAS1 | Hordeum vulgare |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Hordeum vulgare | under normal growth conditions HvAS1 gene seems to be important in roots where nitrogen is assimilated into asparagine for long-distance transport within the plant | ? | - |
? | |
additional information | Hordeum vulgare | under normal growth conditions HvAS2 acts as a housekeeping gene in the leaves | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hordeum vulgare | Q84LA5 | cv. Alexis, HvAS2, two AS genes: HvAS1 and HvAS2 | - |
Hordeum vulgare | Q93XP9 | cv. Alexis, HvAS1, two AS genes: HvAS1 and HvAS2 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | no posttranslational regulation of HvAS1 | Hordeum vulgare |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | expression of HvAS1, higher mRNA levels in younger leaves than in older leaves, induced by dark treatment, induction seems to require a dramatic change in the C/N ratio since no diurnal variation is observed and up-regulation of transcription only occurs after 10 h of darkness | Hordeum vulgare | - |
leaf | expression of HvAS2 | Hordeum vulgare | - |
additional information | expression pattern, dark treatment of plants increases expression of HvAS1 | Hordeum vulgare | - |
additional information | expression pattern, no expression of HvAS2 in roots | Hordeum vulgare | - |
root | low-level expression of HvAS1, unaffected by light | Hordeum vulgare | - |
stem | expression of HvAS1 | Hordeum vulgare | - |
stem | expression of HvAS2 | Hordeum vulgare | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate + L-glutamine | - |
Hordeum vulgare | AMP + diphosphate + L-asparagine + L-glutamate | - |
? | |
additional information | under normal growth conditions HvAS1 gene seems to be important in roots where nitrogen is assimilated into asparagine for long-distance transport within the plant | Hordeum vulgare | ? | - |
? | |
additional information | under normal growth conditions HvAS2 acts as a housekeeping gene in the leaves | Hordeum vulgare | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 65200, HvAS2, amino acid sequence calculation | Hordeum vulgare |
? | x * 65500, amino acid sequence calculation, x * 65000, Western blot analysis, HvAS1 | Hordeum vulgare |
Synonyms | Comment | Organism |
---|---|---|
AS | - |
Hordeum vulgare |
HvAS1 protein | Hordeum vulgare | Hordeum vulgare |
HvAS2 protein | Hordeum vulgare | Hordeum vulgare |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Hordeum vulgare | HvAS2, theoretical pI | - |
5.92 |
Hordeum vulgare | HvAS1, theoretical pI | - |
6.14 |