Crystallization (Comment) | Organism |
---|---|
X-ray crystal structure of AS-B complexed with glutamine and AMP | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic mechanism, kinetic model | Escherichia coli | |
0.26 | - |
ATP | - |
Escherichia coli | |
0.69 | - |
L-glutamine | glutamine-dependent synthetase activity | Escherichia coli | |
0.85 | - |
L-aspartate | - |
Escherichia coli | |
1.67 | - |
L-glutamine | glutaminase activity | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate + L-glutamine | Escherichia coli | glutamine is the in vivo nitrogen source | AMP + diphosphate + L-asparagine + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate | mechanism | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate + L-glutamine | ATP-dependent, mechanism including an enzyme-ATP-Asp-Gln quarternary complex, AS-B structure, two active sites | Escherichia coli | AMP + diphosphate + L-asparagine + L-glutamate | - |
ir | |
ATP + L-aspartate + L-glutamine | glutamine is the in vivo nitrogen source | Escherichia coli | AMP + diphosphate + L-asparagine + L-glutamate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.56 | - |
L-aspartate | - |
Escherichia coli | |
2.18 | - |
ATP | - |
Escherichia coli | |
2.73 | - |
L-glutamine | glutamine-dependent synthetase activity | Escherichia coli | |
2.94 | - |
ATP | - |
Escherichia coli | |
2.94 | - |
L-aspartate | - |
Escherichia coli | |
3 | 6 | L-glutamine | glutaminase activity | Escherichia coli | |
3 | 6 | L-glutamine | glutamine-dependent synthetase activity | Escherichia coli | |
3.38 | - |
L-glutamine | glutaminase activity | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Escherichia coli |