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Literature summary for 6.3.5.4 extracted from

  • Ta, T.C.; MacDowall, F.D.H.; Faris, M.A.
    Asparagine synthetase from root nodules of alfalfa (1989), Biochem. Cell Biol., 67, 455-459.
No PubMed abstract available
Search for more literature for 6.3.5.4

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.16
-
 Gln
-
 Medicago sativa
2.7
-
 NH4+
-
 Medicago sativa

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Medicago sativa 5737
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Medicago sativa Asn, the end product of the mass action of symbiotic NH4+ synthesis, is the principal N-transport-compound of many temperate legumes ?
-
 ?

Organism

Organism UniProt Comment Textmining
Medicago sativa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Medicago sativa

Source Tissue

Source Tissue Comment Organism Textmining
root nodule
-
 Medicago sativa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Medicago sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Asp + L-Gln the ratio of Gln- to NH4+-dependent activity is 2.5 Medicago sativa AMP + diphosphate + Asn + Glu
-
 ?
ATP + L-Asp + NH3 the ratio of Gln-dependent to NH4+-dependent activity is 2.5 Medicago sativa AMP + diphosphate + Asn
-
 ?
additional information Asn, the end product of the mass action of symbiotic NH4+ synthesis, is the principal N-transport-compound of many temperate legumes Medicago sativa ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8 8.2
-
 Medicago sativa