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Literature summary for 6.3.5.2 extracted from

  • Oliver, J.C.; Gudihal, R.; Burgner, J.W.; Pedley, A.M.; Zwierko, A.T.; Davisson, V.J.; Linger, R.S.
    Conformational changes involving ammonia tunnel formation and allosteric control in GMP synthetase (2014), Arch. Biochem. Biophys., 545, 22-32 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.5.2

Crystallization (Commentary)

Crystallization (Comment) Organism
structural model of GMPS in a closed, active state. The salt bridge between residues H186 and E383 functions as a connection between the two active sites Escherichia coli

Protein Variants

Protein Variants Comment Organism
E383A 8fold decrease in glutaminase activity Escherichia coli
H186A 30-50 fold decrease in Km value for glutamine Escherichia coli
H186A/E383A mutant exhibits poor solubility and basal glutaminase activity Escherichia coli
H186E/E383H interchange of the amino acids of the salt bridge, significant decrease in catalytic activity Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
 XMP mutant H186A/E383A, overall reaction, pH 8.5, 37°C Escherichia coli
0.008
-
 XMP mutant H186E/E383H, overall reaction, pH 8.5, 37°C Escherichia coli
0.022
-
 XMP mutant E383A, overall reaction, pH 8.5, 37°C Escherichia coli
0.043
-
 XMP wild-type, release of NH3, pH 8.5, 37°C Escherichia coli
0.045
-
 XMP mutant H186A, overall reaction, pH 8.5, 37°C Escherichia coli
0.05
-
 XMP mutant H186A, release of NH3, pH 8.5, 37°C Escherichia coli
0.05
-
 XMP mutant H186E/E383H, release of NH3, pH 8.5, 37°C Escherichia coli
0.053
-
 XMP wild-type, overall reaction, pH 8.5, 37°C Escherichia coli
0.056
-
 XMP mutant E383A, release of NH3, pH 8.5, 37°C Escherichia coli
0.074
-
 XMP mutant H186A/E383A, release of NH3, pH 8.5, 37°C Escherichia coli
1.6
-
 L-glutamine wild-type, pH 8.5, 37°C Escherichia coli
3.2
-
 L-glutamine mutant E383A, pH 8.5, 37°C Escherichia coli
67
-
 L-glutamine mutant H186E/E383H, pH 8.5, 37°C Escherichia coli
80
-
 L-glutamine mutant H186A, pH 8.5, 37°C Escherichia coli
99
-
 L-glutamine mutant H186A/E383A, pH 8.5, 37°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P04079
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + XMP + L-glutamine + H2O
-
 Escherichia coli AMP + diphosphate + GMP + L-glutamate
-
 ?
ATP + XMP + NH3
-
 Escherichia coli AMP + diphosphate + GMP
-
 ?
L-glutamine + H2O
-
 Escherichia coli L-glutamate + NH3
-
 ?

Synonyms

Synonyms Comment Organism
GMPS
-
 Escherichia coli
GuaA
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.081
-
 XMP mutant H186E/E383H, overall reaction, pH 8.5, 37°C Escherichia coli
0.085
-
 XMP mutant H186A/E383A, overall reaction, pH 8.5, 37°C Escherichia coli
3.2
-
 XMP mutant E383A, overall reaction, pH 8.5, 37°C Escherichia coli
3.2
-
 L-glutamine mutant E383A, pH 8.5, 37°C Escherichia coli
4.7
-
 L-glutamine mutant H186E/E383H, pH 8.5, 37°C Escherichia coli
4.8
-
 L-glutamine mutant H186A/E383A, pH 8.5, 37°C Escherichia coli
6.1
-
 XMP mutant H186E/E383H, release of NH3, pH 8.5, 37°C Escherichia coli
7
-
 XMP mutant E383A, release of NH3, pH 8.5, 37°C Escherichia coli
9
-
 XMP mutant H186A/E383A, release of NH3, pH 8.5, 37°C Escherichia coli
13.3
-
 XMP wild-type, release of NH3, pH 8.5, 37°C Escherichia coli
13.7
-
 L-glutamine wild-type, pH 8.5, 37°C Escherichia coli
14
-
 XMP mutant H186A, overall reaction, pH 8.5, 37°C Escherichia coli
15.6
-
 XMP mutant H186A, release of NH3, pH 8.5, 37°C Escherichia coli
16
-
 L-glutamine mutant H186A, pH 8.5, 37°C Escherichia coli
23
-
 XMP wild-type, overall reaction, pH 8.5, 37°C Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.049
-
 L-glutamine mutant H186A/E383A, pH 8.5, 37°C Escherichia coli
0.07
-
 L-glutamine mutant H186E/E383H, pH 8.5, 37°C Escherichia coli
0.21
-
 L-glutamine mutant H186A, pH 8.5, 37°C Escherichia coli
1
-
 L-glutamine mutant E383A, pH 8.5, 37°C Escherichia coli
8
-
 L-glutamine wild-type, pH 8.5, 37°C Escherichia coli
100
-
 XMP mutant H186E/E383H, overall reaction, pH 8.5, 37°C Escherichia coli
110
-
 XMP mutant H186A/E383A, overall reaction, pH 8.5, 37°C Escherichia coli
120
-
 XMP mutant E383A, release of NH3, pH 8.5, 37°C Escherichia coli
120
-
 XMP mutant H186A/E383A, release of NH3, pH 8.5, 37°C Escherichia coli
120
-
 XMP mutant H186E/E383H, release of NH3, pH 8.5, 37°C Escherichia coli
140
-
 XMP mutant E383A, overall reaction, pH 8.5, 37°C Escherichia coli
310
-
 XMP mutant H186A, overall reaction, pH 8.5, 37°C Escherichia coli
310
-
 XMP mutant H186A, release of NH3, pH 8.5, 37°C Escherichia coli
310
-
 XMP wild-type, release of NH3, pH 8.5, 37°C Escherichia coli
430
-
 XMP wild-type, overall reaction, pH 8.5, 37°C Escherichia coli