Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.3.5.2 extracted from

  • Bhat, J.Y.; Shastri, B.G.; Balaram, H.
    Kinetic and biochemical characterization of Plasmodium falciparum guanosine 5-monophosphate synthetase (2007), Biochem. J., 409, 263-273.
    View publication on PubMed
Search for more literature for 6.3.5.2

Cloned(Commentary)

Cloned (Comment) Organism
-
 Plasmodium falciparum

Inhibitors

Inhibitors Comment Organism Structure
6-thioguanine 0.5 mM, 27% inhibition Plasmodium falciparum
8-Azaguanine 0.5 mM, 40% inhibition Plasmodium falciparum
adenosine 0.5 mM, 37% inhibition Plasmodium falciparum
adenosine 5'-(beta,gamma-imido)triphosphate non-competitive versus XMP, competitive versus ATP Plasmodium falciparum
AMP 0.5 mM, 22% inhibitiion; 0.5 mM, 22% inhibition Plasmodium falciparum
AMP-PNP potent inhibitor, competitive and non-competitive inhibitor with respect to ATP and XMP Plasmodium falciparum
diphosphate non-competitive versus XMP, competitive versus ATP, non-competitive versus L-Gln; potent inhibitor, competitive and non-competitive inhibition with respect to ATP and XMP Plasmodium falciparum
GDP 0.5 mM, 42% inhibition Plasmodium falciparum
GMP competitive versus XMP, uncompetitive versus ATP, non-competitive versus L-Gln; potent inhibitor, competitive and uncompetitive inhibition with respect to XMP and ATP Plasmodium falciparum
GTP 0.5 mM, 40% inhibition Plasmodium falciparum
guanosine 0.5 mM, 50% inhibition Plasmodium falciparum
IMP 0.5 mM, 10% inhibition Plasmodium falciparum
K+
-
 Plasmodium falciparum
Li+
-
 Plasmodium falciparum
additional information not inhibitory at 0.5 mM: hypoxanthine, chloroadenosine, xanthosine, inosine, decoyinine Plasmodium falciparum
Na+ 25% loss of activity at 24 mM, 90% loss of activity at 200 mM Plasmodium falciparum
phosphate
-
 Plasmodium falciparum
Psicofuranine 0.5 mM, 25% inhibition Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0168
-
 XMP pH 8.5, 37°C Plasmodium falciparum
0.0168
-
 XMP 37°C, pH 8.5 Plasmodium falciparum
0.26
-
 ATP pH 8.5, 37°C Plasmodium falciparum
0.26
-
 ATP 37°C, pH 8.5 Plasmodium falciparum
0.472
-
 L-Gln pH 8.5, 37°C Plasmodium falciparum
0.472
-
 L-glutamine 37°C, pH 8.5 Plasmodium falciparum
5.4
-
 NH4+ 37°C, pH 8.5 Plasmodium falciparum

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ replacement of Mg2+ with Mn2+ leads to 85% drop in activity Plasmodium falciparum
Mg2+ required for catalysis Plasmodium falciparum
Mg2+ half-maximal activity at 2.1 mM, Hill-coefficient of 4.4 Plasmodium falciparum
Mn2+ replacement of Mg2+ with Mn2+ leads to 70% drop in activity Plasmodium falciparum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 x * 65000, SDS-PAGE Plasmodium falciparum
160000
-
 gel filtration Plasmodium falciparum

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Plasmodium falciparum

Reaction

Reaction Comment Organism Reaction ID
ATP + XMP + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate steady-state ordered binding of ATP followed by XMP to the ATPase domain with glutamine binding in a random manner to the GAT domain Plasmodium falciparum
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + xanthosine 5'-phosphate + L-glutamine + H2O steady state ordered binding of the substrates ATP and XMP to the ATPase domain, with ATP being the first substrate to bind followed by XMP Plasmodium falciparum AMP + diphosphate + GMP + L-glutamate
-
 ?
ATP + XMP + L-Gln
-
 Plasmodium falciparum AMP + diphosphate + GMP + L-Glu
-
 ?
ATP + XMP + NH4+ NH4+ in form of (NH4)2SO4 Plasmodium falciparum AMP + diphosphate + GMP
-
 ?

Subunits

Subunits Comment Organism
? x * 65000, SDS-PAGE Plasmodium falciparum

Synonyms

Synonyms Comment Organism
GMPS
-
 Plasmodium falciparum
Guanosine 5-monophosphate synthetase
-
 Plasmodium falciparum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.43
-
 XMP pH 8.5, 37°C Plasmodium falciparum
0.43
-
 ATP 37°C, pH 8.5 Plasmodium falciparum
0.43
-
 L-glutamine 37°C, pH 8.5 Plasmodium falciparum
0.43
-
 XMP 37°C, pH 8.5 Plasmodium falciparum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0364
-
 diphosphate 37°C, pH 8.5, with ATP as variable substrate Plasmodium falciparum
0.0364
-
 diphosphate substrate ATP, pH 8.5, 37°C Plasmodium falciparum
0.038
-
 GMP 37°C, pH 8.5, with XMP as variable substrate Plasmodium falciparum
0.038
-
 GMP substrate XMP, pH 8.5, 37°C Plasmodium falciparum
0.179
-
 GMP 37°C, pH 8.5, with ATP as variable substrate Plasmodium falciparum
0.179
-
 GMP substrate ATP, pH 8.5, 37°C Plasmodium falciparum
0.21
-
 AMP-PNP 37°C, pH 8.5, with ATP as variable substrate Plasmodium falciparum
0.21
-
 adenosine 5'-(beta,gamma-imido)triphosphate substrate ATP, pH 8.5, 37°C Plasmodium falciparum
0.39
-
 GMP 37°C, pH 8.5, with glutamine as variable substrate Plasmodium falciparum
0.39
-
 GMP substrate L-Gln, pH 8.5, 37°C Plasmodium falciparum
0.443
-
 diphosphate 37°C, pH 8.5, with XMP as variable substrate Plasmodium falciparum
0.443
-
 diphosphate substrate XMP, pH 8.5, 37°C Plasmodium falciparum
0.611
-
 AMP-PNP 37°C, pH 8.5, with XMP as variable substrate Plasmodium falciparum
0.611
-
 adenosine 5'-(beta,gamma-imido)triphosphate substrate XMP, pH 8.5, 37°C Plasmodium falciparum
0.933
-
 diphosphate substrate L-Gln, pH 8.5, 37°C Plasmodium falciparum
933
-
 diphosphate 37°C, pH 8.5, with glutamine as variable substrate Plasmodium falciparum

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.5
-
 37°C, pH 8.5 Plasmodium falciparum guanosine
2.7
-
 37°C, pH 8.5 Plasmodium falciparum phosphate