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Literature summary for 6.3.5.13 extracted from

  • Morlot, C.; Straume, D.; Peters, K.; Hegnar, O.; Simon, N.; Villard, A.; Contreras-Martel, C.; Leisico, F.; Breukink, E.; Gravier-Pelletier, C.; Le Corre, L.; Vollmer, W.; Pietrancosta, N.; Havarstein, L.; Zapun, A.
    Structure of the essential peptidoglycan amidotransferase MurT/GatD complex from Streptococcus pneumoniae (2018), Nat. Commun., 9, 3180 .
    View publication on PubMedView publication on EuropePMC
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Crystallization (Commentary)

Crystallization (Comment) Organism
structure of the MurT/GatD complex at 3.0 A resolution. MurT has central and C-terminal domains similar to Mur ligases with a cysteine-rich insertion, which probably binds zinc, contributing to the interface with GatD. GatD is a glutaminase providing ammonia that is likely channeled to the MurT active site through a cavity network Streptococcus pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
 ATP pH 7.5, 30°C Streptococcus pneumoniae
0.078
-
 L-glutamine pH 7.5, 30°C Streptococcus pneumoniae
0.22
-
 beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-ditrans,octacis-undecaprenol pH 7.5, 30°C Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-ditrans,octacis-undecaprenol + L-glutamine + H2O Streptococcus pneumoniae
-
 ADP + phosphate + beta-D-GlcNAc-(1->4)-MurNAc-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenol + L-glutamate
-
 ?
ATP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-ditrans,octacis-undecaprenol + L-glutamine + H2O Streptococcus pneumoniae ATCC BAA-255
-
 ADP + phosphate + beta-D-GlcNAc-(1->4)-MurNAc-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenol + L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Streptococcus pneumoniae Q8DNZ9 and Q8DNZ8 Q8DNZ9 i.e. subunit MurT, Q8DNZ8 i.e. subunit GatD
-
Streptococcus pneumoniae ATCC BAA-255 Q8DNZ9 and Q8DNZ8 Q8DNZ9 i.e. subunit MurT, Q8DNZ8 i.e. subunit GatD
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-ditrans,octacis-undecaprenol + L-glutamine + H2O
-
 Streptococcus pneumoniae ADP + phosphate + beta-D-GlcNAc-(1->4)-MurNAc-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenol + L-glutamate
-
 ?
ATP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-ditrans,octacis-undecaprenol + L-glutamine + H2O
-
 Streptococcus pneumoniae ATCC BAA-255 ADP + phosphate + beta-D-GlcNAc-(1->4)-MurNAc-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenol + L-glutamate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4
-
 beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-ditrans,octacis-undecaprenol pH 7.5, 30°C Streptococcus pneumoniae