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Literature summary for 6.3.5.1 extracted from

  • LaRonde-LeBlanc, N.; Resto, M.; Gerratana, B.
    Regulation of active site coupling in glutamine-dependent NAD(+) synthetase (2009), Nat. Struct. Mol. Biol., 16, 421-429.
    View publication on PubMed
Search for more literature for 6.3.5.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment) Organism
native enzyme and selenomethionine-derivative, to 2.35 and 3.0 A resolution, respectively. Homooctameric subunit organization suggesting a tight dependence of catalysis on the quaternary structure, a 40 A intersubunit ammonia tunnel and structural elements that may be involved in the transfer of information between catalytic sites Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
C176A activity smilar to wild-type Mycobacterium tuberculosis
D656A strong decrease in catalytic efficiency Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.089
-
 NH3 mutant D656A, ammonia-dependent reaction Mycobacterium tuberculosis
0.12
-
 ATP wild-type, glutamine-dependent reaction Mycobacterium tuberculosis
0.13
-
 deamido-NAD+ wild-type, glutamine-dependent reaction Mycobacterium tuberculosis
0.6
-
 ATP mutant C176A, ammonia-dependent reaction Mycobacterium tuberculosis
0.65
-
 deamido-NAD+ mutant C176A, ammonia-dependent reaction Mycobacterium tuberculosis
0.7
-
 deamido-NAD+ wild-type, ammonia-dependent reaction Mycobacterium tuberculosis
1.3
-
 L-Gln wild-type, glutamine-dependent reaction Mycobacterium tuberculosis
1.4
-
 ATP wild-type, ammonia-dependent reaction Mycobacterium tuberculosis
7.8
-
 L-Gln mutant D656A, glutamine-dependent reaction Mycobacterium tuberculosis
15
-
 NH3 mutant C176A, ammonia-dependent reaction Mycobacterium tuberculosis
20
-
 L-Gln wild-type, ammonia-dependent reaction Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + deamido-NAD+ + L-Gln
-
 Mycobacterium tuberculosis AMP + diphosphate + NAD+ + L-Glu
-
 ?
ATP + deamido-NAD+ + NH3 + H2O
-
 Mycobacterium tuberculosis AMP + diphosphate + NAD+
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0045
-
 NH3 mutant D656A, ammonia-dependent reaction Mycobacterium tuberculosis
0.012
-
 L-Gln mutant D656A, glutamine-dependent reaction Mycobacterium tuberculosis
0.5
-
 deamido-NAD+ wild-type, glutamine-dependent reaction Mycobacterium tuberculosis
0.55
-
 L-Gln wild-type, glutamine-dependent reaction Mycobacterium tuberculosis
0.6
-
 ATP wild-type, glutamine-dependent reaction Mycobacterium tuberculosis
2.6
-
 deamido-NAD+ wild-type, ammonia-dependent reaction Mycobacterium tuberculosis
2.9
-
 ATP wild-type, ammonia-dependent reaction Mycobacterium tuberculosis
3
-
 ATP mutant C176A, ammonia-dependent reaction Mycobacterium tuberculosis
3
-
 L-Gln wild-type, ammonia-dependent reaction Mycobacterium tuberculosis
3.3
-
 NH3 mutant C176A, ammonia-dependent reaction Mycobacterium tuberculosis
3.7
-
 deamido-NAD+ mutant C176A, ammonia-dependent reaction Mycobacterium tuberculosis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0015
-
 L-Glu mutant D656A, glutamine-dependent reaction Mycobacterium tuberculosis
0.051
-
 NH3 mutant D656A, ammonia-dependent reaction Mycobacterium tuberculosis
0.15
-
 L-Glu wild-type, ammonia-dependent reaction Mycobacterium tuberculosis
0.22
-
 NH3 mutant C176A, ammonia-dependent reaction Mycobacterium tuberculosis
0.42
-
 L-Glu wild-type, glutamine-dependent reaction Mycobacterium tuberculosis
2.1
-
 ATP wild-type, ammonia-dependent reaction Mycobacterium tuberculosis
3.7
-
 deamido-NAD+ wild-type, ammonia-dependent reaction Mycobacterium tuberculosis
3.8
-
 deamido-NAD+ wild-type, glutamine-dependent reaction Mycobacterium tuberculosis
5
-
 ATP mutant C176A, ammonia-dependent reaction Mycobacterium tuberculosis
5
-
 ATP wild-type, glutamine-dependent reaction Mycobacterium tuberculosis
5.7
-
 deamido-NAD+ mutant C176A, ammonia-dependent reaction Mycobacterium tuberculosis