Activating Compound | Comment | Organism | Structure |
---|---|---|---|
nicotinic acid adenine dinucleotide | stimulates activity 50fold | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
D593A | poor synthetase activity | Saccharomyces cerevisiae |
D593A/F622A | synthetase dead | Saccharomyces cerevisiae |
E177A | poor synthetase activity | Saccharomyces cerevisiae |
F622A | poor synthetase activity | Saccharomyces cerevisiae |
I111A | poor synthetase activity | Saccharomyces cerevisiae |
L529A/L604A | poor synthetase activity | Saccharomyces cerevisiae |
L604A | poor synthetase activity | Saccharomyces cerevisiae |
L604N | poor synthetase activity | Saccharomyces cerevisiae |
M621A | synthetase mutant that depresses all activities | Saccharomyces cerevisiae |
R112L | poor synthetase activity that inhibit substrate synergism | Saccharomyces cerevisiae |
R112S | poor synthetase activity that inhibit substrate synergism | Saccharomyces cerevisiae |
Y532A/Y601A | synthetase dead | Saccharomyces cerevisiae |
Y601A | poor synthetase activity | Saccharomyces cerevisiae |
Y601A/M621A | synthetase dead | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | - |
L-glutamine | mutant enzyme Y601A, at 37°C | Saccharomyces cerevisiae | |
3.5 | - |
L-glutamine | mutant enzyme Y532A/Y601A, at 37°C | Saccharomyces cerevisiae | |
5.3 | - |
L-glutamine | wild type enzyme, at 37°C | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0002 | - |
mutant enzyme R112L, at 37°C, with 2 mM ATP, 5 mM MgCl2, 50 mM Tris-HCl, pH 8.0, 56 mM KCl, and 0.2 mg/ml bovine serum albumin | Saccharomyces cerevisiae |
0.0011 | - |
mutant enzyme D593A/F622A, at 37°C, with 2 mM ATP, 5 mM MgCl2, 50 mM Tris-HCl, pH 8.0, 56 mM KCl, and 0.2 mg/ml bovine serum albumin | Saccharomyces cerevisiae |
0.0017 | - |
mutant enzyme Y532A/M621A, at 37°C, with 2 mM ATP, 5 mM MgCl2, 50 mM Tris-HCl, pH 8.0, 56 mM KCl, and 0.2 mg/ml bovine serum albumin | Saccharomyces cerevisiae |
0.0017 | - |
mutant enzyme Y532A/Y601A, at 37°C, with 2 mM ATP, 5 mM MgCl2, 50 mM Tris-HCl, pH 8.0, 56 mM KCl, and 0.2 mg/ml bovine serum albumin | Saccharomyces cerevisiae |
0.0087 | - |
mutant enzyme L604N, at 37°C, with 2 mM ATP, 5 mM MgCl2, 50 mM Tris-HCl, pH 8.0, 56 mM KCl, and 0.2 mg/ml bovine serum albumin | Saccharomyces cerevisiae |
0.015 | - |
mutant enzyme L604A/L519A, at 37°C, with 2 mM ATP, 5 mM MgCl2, 50 mM Tris-HCl, pH 8.0, 56 mM KCl, and 0.2 mg/ml bovine serum albumin | Saccharomyces cerevisiae |
0.024 | - |
mutant enzyme R112S, at 37°C, with 2 mM ATP, 5 mM MgCl2, 50 mM Tris-HCl, pH 8.0, 56 mM KCl, and 0.2 mg/ml bovine serum albumin | Saccharomyces cerevisiae |
0.0533 | - |
mutant enzyme I111A, at 37°C, with 2 mM ATP, 5 mM MgCl2, 50 mM Tris-HCl, pH 8.0, 56 mM KCl, and 0.2 mg/ml bovine serum albumin | Saccharomyces cerevisiae |
0.0931 | - |
mutant enzyme Y601A, at 37°C, with 2 mM ATP, 5 mM MgCl2, 50 mM Tris-HCl, pH 8.0, 56 mM KCl, and 0.2 mg/ml bovine serum albumin | Saccharomyces cerevisiae |
0.112 | - |
mutant enzyme E177A, at 37°C, with 2 mM ATP, 5 mM MgCl2, 50 mM Tris-HCl, pH 8.0, 56 mM KCl, and 0.2 mg/ml bovine serum albumin | Saccharomyces cerevisiae |
0.288 | - |
mutant enzyme F622A, at 37°C, with 2 mM ATP, 5 mM MgCl2, 50 mM Tris-HCl, pH 8.0, 56 mM KCl, and 0.2 mg/ml bovine serum albumin | Saccharomyces cerevisiae |
0.293 | - |
mutant enzyme L604A, at 37°C, with 2 mM ATP, 5 mM MgCl2, 50 mM Tris-HCl, pH 8.0, 56 mM KCl, and 0.2 mg/ml bovine serum albumin | Saccharomyces cerevisiae |
0.435 | - |
mutant enzyme D593A, at 37°C, with 2 mM ATP, 5 mM MgCl2, 50 mM Tris-HCl, pH 8.0, 56 mM KCl, and 0.2 mg/ml bovine serum albumin | Saccharomyces cerevisiae |
0.644 | - |
mutant enzyme M621A, at 37°C, with 2 mM ATP, 5 mM MgCl2, 50 mM Tris-HCl, pH 8.0, 56 mM KCl, and 0.2 mg/ml bovine serum albumin | Saccharomyces cerevisiae |
2.33 | - |
wild type enzyme, at 37°C, with 2 mM ATP, 5 mM MgCl2, 50 mM Tris-HCl, pH 8.0, 56 mM KCl, and 0.2 mg/ml bovine serum albumin | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + deamido-NAD+ + L-glutamine + H2O | - |
Saccharomyces cerevisiae | AMP + diphosphate + NAD+ + L-glutamate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glutamine-dependent NAD+ synthetase | - |
Saccharomyces cerevisiae |
Qns1 | couples a glutamine amidotransferase domain to a second active site that requires ammonia as a reactant | Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
L-glutamine | mutant enzyme Y532A/Y601A, at 37°C | Saccharomyces cerevisiae | |
0.16 | - |
L-glutamine | mutant enzyme Y601A, at 37°C | Saccharomyces cerevisiae | |
6.4 | - |
L-glutamine | wild type enzyme, at 37°C | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Saccharomyces cerevisiae |