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Literature summary for 6.3.4.6 extracted from

  • Zhao, J.; Zhu, L.; Fan, C.; Wu, Y.; Xiang, S.
    Structure and function of urea amidolyase (2018), Biosci. Rep., 38, pii: BSR20171617.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Kluyveromyces lactis

Crystallization (Commentary)

Crystallization (Comment) Organism
deletion mutant lacking the biotin-carboxyl carrier protein domain, to 6.5 A resolution. Space group P212121, with four monomers per asymmetric unit Kluyveromyces lactis

Protein Variants

Protein Variants Comment Organism
G559E/G572E mutation renders the protein monomeric Kluyveromyces lactis
K1605A mutation in active site of the carboxylyase. A mixture of mutants K1605A and S177A is as efficient as wild-type Kluyveromyces lactis
S177A mutation in the active site of the allophanate hydrolase. A mixture of mutants K1605A and S177A is as efficient as wild-type Kluyveromyces lactis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.203
-
Urea mutant S177A, pH 8.0, temperature not specified in the publication Kluyveromyces lactis
0.216
-
Urea mixture of mutants S177A and mutant K1605A, pH 8.0, temperature not specified in the publication Kluyveromyces lactis
0.246
-
Urea isolated urea carboxyxlase domain plus allophanate hydrolase domain, pH 8.0, temperature not specified in the publication Kluyveromyces lactis
0.255
-
Urea wild-type, pH 8.0, temperature not specified in the publication Kluyveromyces lactis
0.299
-
Urea mutant K1605A, pH 8.0, temperature not specified in the publication Kluyveromyces lactis
0.361
-
Urea mutant G559E/G572E, pH 8.0, temperature not specified in the publication Kluyveromyces lactis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
500000
-
gel filtration Kluyveromyces lactis

Organism

Organism UniProt Comment Textmining
Kluyveromyces lactis Q6CP22 enzyme is composed of urea carboxylase domain plus allophanate hydrolase domain, EC 3.5.1.54
-
Kluyveromyces lactis DSM 70799 Q6CP22 enzyme is composed of urea carboxylase domain plus allophanate hydrolase domain, EC 3.5.1.54
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + urea + HCO3-
-
Kluyveromyces lactis ADP + phosphate + urea-1-carboxylate
-
?
ATP + urea + HCO3-
-
Kluyveromyces lactis DSM 70799 ADP + phosphate + urea-1-carboxylate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 202000, SDS-PAGE Kluyveromyces lactis
More holo-enzyme formation is essential for optimal activity Kluyveromyces lactis

Synonyms

Synonyms Comment Organism
KLLA0_E08119g
-
Kluyveromyces lactis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.78
-
Urea mutant S177A, pH 8.0, temperature not specified in the publication Kluyveromyces lactis
1.33
-
Urea mutant K1605A, pH 8.0, temperature not specified in the publication Kluyveromyces lactis
3
-
Urea mutant G559E/G572E, pH 8.0, temperature not specified in the publication Kluyveromyces lactis
10.07
-
Urea isolated urea carboxyxlase domain plus allophanate hydrolase domain, pH 8.0, temperature not specified in the publication Kluyveromyces lactis
12.68
-
Urea mixture of mutants S177A and mutant K1605A, pH 8.0, temperature not specified in the publication Kluyveromyces lactis
13.9
-
Urea wild-type, pH 8.0, temperature not specified in the publication Kluyveromyces lactis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.87
-
Urea mutant S177A, pH 8.0, temperature not specified in the publication Kluyveromyces lactis
4.45
-
Urea mutant K1605A, pH 8.0, temperature not specified in the publication Kluyveromyces lactis
8.31
-
Urea mutant G559E/G572E, pH 8.0, temperature not specified in the publication Kluyveromyces lactis
40.9
-
Urea isolated urea carboxyxlase domain plus allophanate hydrolase domain, pH 8.0, temperature not specified in the publication Kluyveromyces lactis
54.6
-
Urea wild-type, pH 8.0, temperature not specified in the publication Kluyveromyces lactis
58.7
-
Urea mixture of mutants S177A and mutant K1605A, pH 8.0, temperature not specified in the publication Kluyveromyces lactis