Literature summary for 6.3.4.5 extracted from

Lakhal-Naouar, I.; Jardim, A.; Strasser, R.; Luo, S.; Kozakai, Y.; Nakhasi, H.L.; Duncan, R.C.
Leishmania donovani argininosuccinate synthase is an active enzyme associated with parasite pathogenesis (2012), PLoS Negl. Trop. Dis., 6, e1849.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 (DE3) pLysS cells	Leishmania donovani

Protein Variants

Protein Variants	Comment	Organism
G128S	inactive	Leishmania donovani

Inhibitors

Inhibitors	Comment	Organism	Structure
N-methylaspartic acid	at 1 mM methyl-DL-aspartic acid the enzyme activity is reduced by about 60%, while at 5 mM the catalytic activity is completely abolished	Leishmania donovani

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	the majority of the enzyme is in the cytosol	Leishmania donovani	5829	-
vesicle	only a small proportion of the total cellular enzyme is localized in vesicles	Leishmania donovani	31982	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
46000	-	x * 46000, calculated from amino acid sequence	Leishmania donovani
49000	-	x * 49000, recombinant enzyme, SDS-PAGE	Leishmania donovani

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + L-citrulline + L-aspartate	Leishmania donovani	-	AMP + diphosphate + 2-(Nomega-L-arginino)succinate	-	?

Organism

Organism	UniProt	Comment	Textmining
Leishmania donovani	H9BG20	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA agarose column chromatography	Leishmania donovani

Source Tissue

Source Tissue	Comment	Organism	Textmining
amastigote	more abundantly expressed in amastigotes than in promastigotes	Leishmania donovani	-
promastigote	-	Leishmania donovani	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-citrulline + L-aspartate	-	Leishmania donovani	AMP + diphosphate + 2-(Nomega-L-arginino)succinate	-	?

Subunits

Subunits	Comment	Organism
?	x * 49000, recombinant enzyme, SDS-PAGE	Leishmania donovani
?	x * 46000, calculated from amino acid sequence	Leishmania donovani

Synonyms

Synonyms	Comment	Organism
ASS	-	Leishmania donovani

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Leishmania donovani

pI Value

Organism	Comment	pI Value Maximum	pI Value
Leishmania donovani	calculated from amino acid sequence	-	5.8

General Information

General Information	Comment	Organism
physiological function	the enzyme is essential for parasite survival and growth in the mammalian host	Leishmania donovani

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Release 2023.1 (January 2023)