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Literature summary for 6.3.4.5 extracted from

  • Goto, M.; Omi, R.; Miyahara, I.; Sugahara, M.; Hirotsu, K.
    Structures of argininosuccinate synthetase in enzyme-ATP substrates and enzyme-AMP product forms. Stereochemistry of the catalytic reaction (2003), J. Biol. Chem., 278, 22964-22971.
    View publication on PubMed
Search for more literature for 6.3.4.5

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3)pLysE Thermus thermophilus

Crystallization (Commentary)

Crystallization (Comment) Organism
structures of enzyme complexed with intact ATP and substrates citrulline and aspartate, complexed with AMP and argininosuccinate, and complexed with AMP-PNP, arginine, aspartate and Mg2+, vapor diffusion method, X-ray analysis Thermus thermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for maximal activity, in octahedral coordination with 3 oxygen atoms of the triphosphate group and 3 water molecules Thermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44815
-
 4 * 44815, recombinant tAsS, subunit/domain structure, amino acid sequence Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-citrulline + L-aspartate Thermus thermophilus seventh step of the arginine biosynthesis, second step of the urea cycle AMP + diphosphate + L-argininosuccinate
-
 ?
ATP + L-citrulline + L-aspartate Thermus thermophilus HB8 / ATCC 27634 / DSM 579 seventh step of the arginine biosynthesis, second step of the urea cycle AMP + diphosphate + L-argininosuccinate
-
 ?

Organism

Organism UniProt Comment Textmining
Thermus thermophilus P59846
-
-
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 P59846
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermus thermophilus

Reaction

Reaction Comment Organism Reaction ID
ATP + L-citrulline + L-aspartate = AMP + diphosphate + 2-(Nomega-L-arginino)succinate detailed catalytic mechanism, stereochemistry Thermus thermophilus
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.06
-
 20°C Thermus thermophilus
0.92
-
 70°C Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-citrulline + L-aspartate ATP-dependent condensation of citrulline with aspartate, detailed mechanism via citrullyl-AMP intermediate, stereochemistry of catalysis, no conformational change upon binding of ATP, active site structure Thermus thermophilus AMP + diphosphate + L-argininosuccinate
-
 r
ATP + L-citrulline + L-aspartate seventh step of the arginine biosynthesis, second step of the urea cycle Thermus thermophilus AMP + diphosphate + L-argininosuccinate
-
 ?
ATP + L-citrulline + L-aspartate ATP-dependent condensation of citrulline with aspartate, detailed mechanism via citrullyl-AMP intermediate, stereochemistry of catalysis, no conformational change upon binding of ATP, active site structure Thermus thermophilus HB8 / ATCC 27634 / DSM 579 AMP + diphosphate + L-argininosuccinate
-
 r
ATP + L-citrulline + L-aspartate seventh step of the arginine biosynthesis, second step of the urea cycle Thermus thermophilus HB8 / ATCC 27634 / DSM 579 AMP + diphosphate + L-argininosuccinate
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 44815, recombinant tAsS, subunit/domain structure, amino acid sequence Thermus thermophilus

Synonyms

Synonyms Comment Organism
Argininosuccinate synthetase
-
 Thermus thermophilus
ASS
-
 Thermus thermophilus
tAsS
-
 Thermus thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 assay at Thermus thermophilus