Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-Arg | non-competitive with respect to Asp and MgATP2- | Saccharomyces cerevisiae | |
L-argininosuccinate | - |
Saccharomyces cerevisiae | |
phosphate | competitive with respect to citrulline and Asp | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
4 * 49000, SDS-PAGE in presence of 2-mercaptoethanol | Saccharomyces cerevisiae |
228000 | - |
gel filtration | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-citrulline + L-Asp | Saccharomyces cerevisiae | anabolic function in biosynthesis of Arg and catabolic function as the first enzyme of citrulline utilization as nitrogen source | ? | - |
? | |
ATP + L-citrulline + L-Asp | Saccharomyces cerevisiae 1278b | anabolic function in biosynthesis of Arg and catabolic function as the first enzyme of citrulline utilization as nitrogen source | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
mutant MG409 | - |
Saccharomyces cerevisiae 1278b | - |
mutant MG409 | - |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + L-citrulline + L-aspartate = AMP + diphosphate + 2-(Nomega-L-arginino)succinate | rapid-equilibrium random mechanism | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.54 | - |
- |
Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
-20°C, stable for months | Saccharomyces cerevisiae |
0-4°C, without (NH4)2SO4, stable for about 2 weeks | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-citrulline + L-Asp | r | Saccharomyces cerevisiae | AMP + diphosphate + L-argininosuccinate | - |
? | |
ATP + L-citrulline + L-Asp | r | Saccharomyces cerevisiae 1278b | AMP + diphosphate + L-argininosuccinate | - |
? | |
ATP + L-citrulline + L-Asp | anabolic function in biosynthesis of Arg and catabolic function as the first enzyme of citrulline utilization as nitrogen source | Saccharomyces cerevisiae | ? | - |
? | |
ATP + L-citrulline + L-Asp | anabolic function in biosynthesis of Arg and catabolic function as the first enzyme of citrulline utilization as nitrogen source | Saccharomyces cerevisiae 1278b | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | , 4 * 49000, SDS-PAGE in presence of 2-mercaptoethanol | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 6.5 | reaction of AMP + diphosphate + L-argininosuccinate | Saccharomyces cerevisiae |
7.5 | 7.8 | reaction of ATP + citrulline + L-argininosuccinate | Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7 | about 60% of maximal activity at pH 5.5 and 7 | Saccharomyces cerevisiae |
6.5 | 8.5 | 6.5: about 55% of maximal activity, 8.5: about 70% of maximal activity, reaction of ATP + citrulline + L-argininosuccinate | Saccharomyces cerevisiae |