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Literature summary for 6.3.4.5 extracted from

  • Saheki, T.; Kusumi, T.; Takada, S.; Katsunuma, T.
    Studies of rat liver argininosuccinate synthetase. I. Physicochemical, catalytic, and immunochemical properties (1977), J. Biochem., 81, 687-696.
    View publication on PubMed
Search for more literature for 6.3.4.5

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoate)
-
 Rattus norvegicus
CTP slight Rattus norvegicus
dGTP slight Rattus norvegicus
dTTP slight Rattus norvegicus
GTP slight Rattus norvegicus
UTP slight Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
 diphosphate
-
 Rattus norvegicus
0.02
-
 citrulline
-
 Rattus norvegicus
0.022
-
 AMP
-
 Rattus norvegicus
0.044
-
 citrulline Asp Rattus norvegicus
0.12
-
 argininosuccinate
-
 Rattus norvegicus
0.15
-
 ATP
-
 Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 4 * 48000, SDS-PAGE, PAGE with 6 M urea Rattus norvegicus
158000
-
 gel filtration Rattus norvegicus
207000
-
 sedimentation equilibrium centrifugation Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Storage Stability

Storage Stability Organism
-20°C, 0.05 M citrulline, 0.05 M Asp, or 0.01 M argininosuccinate, stable for more than 1 year Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + citrulline + aspartate
-
 Rattus norvegicus AMP + diphosphate + 2-(Nomega-L-arginino)succinate
-
 ?
ATP + L-citrulline + L-Asp r Rattus norvegicus AMP + diphosphate + L-argininosuccinate
-
 ?
dATP + L-citrulline + L-Asp 2.6% as active as ATP Rattus norvegicus dAMP + diphosphate + L-argininosuccinate
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 48000, SDS-PAGE, PAGE with 6 M urea Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2 6.3 reaction of AMP + diphosphate + L-argininosuccinate Rattus norvegicus
8.4
-
 reaction of ATP + citrulline + L-Asp Rattus norvegicus