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Literature summary for 6.3.4.5 extracted from
- Enzymes of arginine and urea synthesis (1973), Adv. Enzymol. Relat. Areas Mol. Biol., 39, 1-90.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AMP | competitive with respect to ATP | Bos taurus |  |
| D-Asp | - |
Bos taurus | |
| diphosphate | - |
Bos taurus | |
| erythro-3-Hydroxy-3-methylaspartate | - |
Bos taurus | |
| L-argininosuccinate | competitive with respect to citrulline, Asp, and ATP | Bos taurus | |
| threo-3-Hydroxy-3-methylaspartate | - |
Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.035 | - |
L-Asp | - |
Bos taurus | |
| 0.88 | - |
threo-beta-methyl-L-Asp | - |
Bos taurus | |
| 4.5 | - |
threo-beta-hydroxy-L-Asp | - |
Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
4 * 45000, two pairs of monomers crosslinked by disulfide bonds, SDS-PAGE in presence of reducing agent | Bos taurus |
| 90000 | - |
2 * 90000, SDS-PAGE in absence of reducing agent | Bos taurus |
| 175000 | - |
gel filtration | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + L-citrulline + L-aspartate = AMP + diphosphate + 2-(Nomega-L-arginino)succinate | mechanism | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Bos taurus | - |
| liver | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.83 | - |
- |
Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-citrulline + L-Asp | r | Bos taurus | AMP + diphosphate + L-argininosuccinate | - |
? | |
| ATP + L-citrulline + L-Asp | D-Asp is inactive as substrate | Bos taurus | AMP + diphosphate + L-argininosuccinate | - |
? | |
| ATP + L-citrulline + threo-beta-hydroxy-DL-Asp | - |
Bos taurus | ? | - |
? | |
| ATP + L-citrulline + threo-beta-methyl-L-Asp | - |
Bos taurus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 2 * 90000, SDS-PAGE in absence of reducing agent | Bos taurus |
| tetramer | 4 * 45000, two pairs of monomers crosslinked by disulfide bonds, SDS-PAGE in presence of reducing agent | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
reaction of AMP + diphosphate + L-argininosuccinate | Bos taurus |
| 8.7 | - |
reaction of ATP + L-citulline + L-Asp | Bos taurus |
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Release 2023.1 (January 2023)
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