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Literature summary for 6.3.4.4 extracted from

  • Mehrotra, S.; Balaram, H.
    Kinetic characterization of adenylosuccinate synthetase from the thermophilic archaea Methanocaldococcus jannaschii (2007), Biochemistry, 46, 12821-12832.
    View publication on PubMed
Search for more literature for 6.3.4.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 Methanocaldococcus jannaschii

Inhibitors

Inhibitors Comment Organism Structure
adenylosuccinate competitive inhibitor of IMP, noncompetitive inhibitor of GTP and aspartate Methanocaldococcus jannaschii
AMP competitive with respect to IMP and noncompetitive with GTP and aspartate Methanocaldococcus jannaschii
D-fructose 1,6 bisphosphate competitive with IMP and noncompetitive against GTP Methanocaldococcus jannaschii
GDP competitive inhibitor of GTP, noncompetitive inhibitor with IMP and aspartate Methanocaldococcus jannaschii
GMP competitive with respect to GTP and noncompetitive with IMP and aspartate Methanocaldococcus jannaschii
IMP inhibits the enzyme activity at subsaturating GTP concentrations Methanocaldococcus jannaschii
N-formyl-N-hydroxyglycine i.e. hadacidin. competitive inhibitor of aspartate, showed uncompetitive inhibition with IMP and GTP Methanocaldococcus jannaschii
phosphate potent inhibitor, biphasic inhibition, competitive with IMP and noncompetitive with GTP Methanocaldococcus jannaschii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0426
-
 GTP pH 6.0, 70°C Methanocaldococcus jannaschii
0.0755
-
 IMP pH 6.0, 70°C Methanocaldococcus jannaschii
1.079
-
 L-aspartate pH 6.0, 70°C Methanocaldococcus jannaschii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requires Mg2+ for catalysis with maximum activity obtained at 15 mM magnesium acetate Methanocaldococcus jannaschii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37855
-
 2 * 37855, MALDI mass spectrometric analysis, equilibrium mixture of dimers and tetramers with the tetramer being the catalytically active form. The tetramer dissociates into dimers with a minor increase in ionic strength of the buffer, while the dimer is extremely stable and does not dissociate even at 1.2 M NaCl Methanocaldococcus jannaschii
37855
-
 4 * 37855, MALDI mass spectrometric analysis, equilibrium mixture of dimers and tetramers with the tetramer being the catalytically active form. The tetramer dissociates into dimers with a minor increase in ionic strength of the buffer, while the dimer is extremely stable and does not dissociate even at 1.2 M NaCl Methanocaldococcus jannaschii

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii Q57981
-
-
Methanocaldococcus jannaschii DSM 2661 Q57981
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Methanocaldococcus jannaschii

Reaction

Reaction Comment Organism Reaction ID
GTP + IMP + L-aspartate = GDP + phosphate + N6-(1,2-dicarboxyethyl)-AMP rapid equilibrium random AB steady-state ordered C kinetic mechanism Methanocaldococcus jannaschii
a

Storage Stability

Storage Stability Organism
-20°C, stable without any decrease in activity for at least 6 months Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + IMP + L-aspartate
-
 Methanocaldococcus jannaschii GDP + phosphate + N6-(1,2-dicarboxyethyl)-AMP i.e. adenylosuccinate ?
GTP + IMP + L-aspartate
-
 Methanocaldococcus jannaschii DSM 2661 GDP + phosphate + N6-(1,2-dicarboxyethyl)-AMP i.e. adenylosuccinate ?

Subunits

Subunits Comment Organism
dimer 2 * 37855, MALDI mass spectrometric analysis, equilibrium mixture of dimers and tetramers with the tetramer being the catalytically active form. The tetramer dissociates into dimers with a minor increase in ionic strength of the buffer, while the dimer is extremely stable and does not dissociate even at 1.2 M NaCl Methanocaldococcus jannaschii
tetramer 4 * 37855, MALDI mass spectrometric analysis, equilibrium mixture of dimers and tetramers with the tetramer being the catalytically active form. The tetramer dissociates into dimers with a minor increase in ionic strength of the buffer, while the dimer is extremely stable and does not dissociate even at 1.2 M NaCl Methanocaldococcus jannaschii

Synonyms

Synonyms Comment Organism
AdSS
-
 Methanocaldococcus jannaschii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
 asasy at Methanocaldococcus jannaschii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.45
-
 GTP pH 6.0, 70°C Methanocaldococcus jannaschii
0.45
-
 L-aspartate pH 6.0, 70°C Methanocaldococcus jannaschii
0.45
-
 IMP pH 6.0, 70°C Methanocaldococcus jannaschii
4.2
-
 GTP pH 6.0, 70°C Methanocaldococcus jannaschii
4.2
-
 L-aspartate pH 6.0, 70°C Methanocaldococcus jannaschii
4.2
-
 IMP pH 6.0, 70°C Methanocaldococcus jannaschii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 assay at Methanocaldococcus jannaschii

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.007
-
 GDP pH 6.0, 70°C, competitive inhibition of GTP Methanocaldococcus jannaschii
0.0094
-
 adenylosuccinate pH 6.0, 70°C, competitive inhibition of IMP Methanocaldococcus jannaschii
0.0105
-
 N-formyl-N-hydroxyglycine pH 6.0, 70°C, competitive inhibition of asparate Methanocaldococcus jannaschii
0.0137
-
 GMP pH 6.0, 70°C, noncompetitive inhibition of GTP Methanocaldococcus jannaschii
0.0264
-
 N-formyl-N-hydroxyglycine pH 6.0, 70°C, uncompetitive inhibition of IMP Methanocaldococcus jannaschii
0.0269
-
 N-formyl-N-hydroxyglycine pH 6.0, 70°C, uncompetitive inhibition of GTP Methanocaldococcus jannaschii
0.0426
-
 adenylosuccinate pH 6.0, 70°C, noncompetitive inhibition of aspartate Methanocaldococcus jannaschii
0.0429
-
 adenylosuccinate pH 6.0, 70°C, noncompetitive inhibition of GTP Methanocaldococcus jannaschii
0.0582
-
 AMP pH 6.0, 70°C, competitive inhibition of IMP Methanocaldococcus jannaschii
0.0821
-
 GDP pH 6.0, 70°C, noncompetitive inhibition of aspartate Methanocaldococcus jannaschii
0.0822
-
 GDP pH 6.0, 70°C, noncompetitive inhibition of IMP Methanocaldococcus jannaschii
0.11
-
 GMP pH 6.0, 70°C, noncompetitive inhibition of IMP Methanocaldococcus jannaschii
0.12
-
 GMP pH 6.0, 70°C, noncompetitive inhibition of asparate Methanocaldococcus jannaschii
0.324
-
 AMP pH 6.0, 70°C, noncompetitive inhibition of aspartate Methanocaldococcus jannaschii
0.402
-
 AMP pH 6.0, 70°C, noncompetitive inhibition of GTP Methanocaldococcus jannaschii
1.5
-
 D-fructose 1,6 bisphosphate pH 6.0, 70°C, competitive inhibition of IMP Methanocaldococcus jannaschii
1.9
-
 phosphate pH 6.0, 70°C, competitive inhibition of IMP Methanocaldococcus jannaschii
4.8
-
 D-fructose 1,6 bisphosphate pH 6.0, 70°C, competitive inhibition of GTP Methanocaldococcus jannaschii
8.3
-
 phosphate pH 6.0, 70°C, competitive inhibition of GTP Methanocaldococcus jannaschii