Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
T128A | site-directed mutagenesis | Escherichia coli |
T129A | site-directed mutagenesis | Escherichia coli |
T300A | site-directed mutagenesis | Escherichia coli |
T301A | site-directed mutagenesis | Escherichia coli |
V273A | site-directed mutagenesis | Escherichia coli |
V273N | site-directed mutagenesis | Escherichia coli |
V273T | site-directed mutagenesis | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
fumarate | - |
Escherichia coli | |
Maleate | - |
Escherichia coli | |
succinate | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
IMP | pH 7.7, 25°C, mutant V273A | Escherichia coli | |
0.019 | - |
IMP | pH 7.7, 25°C, mutant T300A | Escherichia coli | |
0.021 | - |
GTP | pH 7.7, 25°C, mutant V273N | Escherichia coli | |
0.024 | - |
IMP | pH 7.7, 25°C, mutant V273N | Escherichia coli | |
0.026 | - |
GTP | pH 7.7, 25°C, wild-type | Escherichia coli | |
0.028 | - |
IMP | pH 7.7, 25°C, wild-type | Escherichia coli | |
0.031 | - |
GTP | pH 7.7, 25°C, mutant V273A | Escherichia coli | |
0.033 | - |
IMP | pH 7.7, 25°C, mutant T300V | Escherichia coli | |
0.043 | - |
IMP | pH 7.7, 25°C, mutant V273T | Escherichia coli | |
0.045 | - |
GTP | pH 7.7, 25°C, mutant V273T | Escherichia coli | |
0.054 | - |
GTP | pH 7.7, 25°C, mutant T128A | Escherichia coli | |
0.056 | - |
GTP | pH 7.7, 25°C, mutant T300V | Escherichia coli | |
0.057 | - |
GTP | pH 7.7, 25°C, mutant T301A, hydroxylamine as substrate | Escherichia coli | |
0.066 | - |
IMP | pH 7.7, 25°C, wild-type, hydroxylamine as substrate | Escherichia coli | |
0.074 | - |
GTP | pH 7.7, 25°C, mutant V273T, hydroxylamine as substrate | Escherichia coli | |
0.077 | - |
IMP | pH 7.7, 25°C, mutant V273T, hydroxylamine as substrate | Escherichia coli | |
0.113 | - |
IMP | pH 7.7, 25°C, mutant V273N, hydroxylamine as substrate | Escherichia coli | |
0.14 | - |
GTP | pH 7.7, 25°C, mutant V273A, hydroxylamine as substrate | Escherichia coli | |
0.14 | - |
IMP | pH 7.7, 25°C, mutant V273A, hydroxylamine as substrate | Escherichia coli | |
0.17 | - |
L-aspartate | pH 7.7, 25°C, mutant T128A | Escherichia coli | |
0.17 | - |
GTP | pH 7.7, 25°C, mutant T300A | Escherichia coli | |
0.18 | - |
GTP | pH 7.7, 25°C, mutant V273N, hydroxylamine as substrate | Escherichia coli | |
0.19 | - |
GTP | pH 7.7, 25°C, wild-type, hydroxylamine as substrate | Escherichia coli | |
0.23 | - |
L-aspartate | pH 7.7, 25°C, wild-type | Escherichia coli | |
0.24 | - |
L-aspartate | pH 7.7, 25°C, mutant T129A | Escherichia coli | |
0.31 | - |
IMP | pH 7.7, 25°C, mutant T128A | Escherichia coli | |
0.32 | - |
IMP | pH 7.7, 25°C, mutant T301A, hydroxylamine as substrate | Escherichia coli | |
0.82 | - |
L-aspartate | pH 7.7, 25°C, mutant T300V | Escherichia coli | |
0.89 | - |
IMP | pH 7.7, 25°C, mutant T129A | Escherichia coli | |
1.7 | - |
GTP | pH 7.7, 25°C, mutant T129A | Escherichia coli | |
3.4 | - |
L-aspartate | pH 7.7, 25°C, mutant V273T | Escherichia coli | |
6.2 | - |
L-aspartate | pH 7.7, 25°C, mutant T300A | Escherichia coli | |
7 | - |
L-aspartate | pH 7.7, 25°C, mutant V273A | Escherichia coli | |
9 | - |
L-aspartate | pH 7.7, 25°C, mutant V273N | Escherichia coli | |
91 | - |
hydroxylamine | pH 7.7, 25°C, mutant T301A | Escherichia coli | |
110 | - |
hydroxylamine | pH 7.7, 25°C, mutant V273N | Escherichia coli | |
140 | - |
hydroxylamine | pH 7.7, 25°C, mutant V273T | Escherichia coli | |
230 | - |
hydroxylamine | pH 7.7, 25°C, wild-type | Escherichia coli | |
255 | - |
hydroxylamine | pH 7.7, 25°C, mutant V273A | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + IMP + L-aspartate | Escherichia coli | governs the first committed step in de novo biosynthesis of AMP plays a significant role in de novo purine nucleotide biosynthesis, the purine nucleotide cycle, and/or salvage pathway for nucleotides | GDP + phosphate + adenylosuccinate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A7D4 | - |
- |
Purification (Comment) | Organism |
---|---|
wild-type and mutant enzymes | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + IMP + hydroxylamine | - |
Escherichia coli | GDP + phosphate + ? | - |
? | |
GTP + IMP + L-aspartate | - |
Escherichia coli | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | governs the first committed step in de novo biosynthesis of AMP plays a significant role in de novo purine nucleotide biosynthesis, the purine nucleotide cycle, and/or salvage pathway for nucleotides | Escherichia coli | GDP + phosphate + adenylosuccinate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
Adenylosuccinate synthetase | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
GTP | pH 7.7, 25°C, mutant V273N | Escherichia coli | |
0.08 | - |
hydroxylamine | pH 7.7, 25°C, mutant V273T | Escherichia coli | |
0.21 | - |
hydroxylamine | pH 7.7, 25°C, mutant V273A | Escherichia coli | |
0.24 | - |
hydroxylamine | pH 7.7, 25°C, mutant T301A | Escherichia coli | |
0.29 | - |
hydroxylamine | pH 7.7, 25°C, mutant V273N | Escherichia coli | |
0.42 | - |
GTP | pH 7.7, 25°C, mutant T129A | Escherichia coli | |
0.46 | - |
GTP | pH 7.7, 25°C, mutant T128A | Escherichia coli | |
0.5 | - |
hydroxylamine | pH 7.7, 25°C, wild-type | Escherichia coli | |
0.64 | - |
GTP | pH 7.7, 25°C, mutant T300A | Escherichia coli | |
1 | - |
GTP | pH 7.7, 25°C, mutant V273A | Escherichia coli | |
1 | - |
GTP | pH 7.7, 25°C, wild-type | Escherichia coli | |
1.99 | - |
GTP | pH 7.7, 25°C, mutant V273T | Escherichia coli | |
2.94 | - |
GTP | pH 7.7, 25°C, mutant V273T | Escherichia coli | |
3.3 | - |
GTP | pH 7.7, 25°C, mutant T300V | Escherichia coli | |
6.08 | - |
GTP | pH 7.7, 25°C, mutant T300A | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
fumarate | pH 7.7, 25°C, mutant V273N | Escherichia coli | |
0.078 | - |
Maleate | pH 7.7, 25°C, mutant V273T | Escherichia coli | |
0.08 | - |
succinate | pH 7.7, 25°C, mutant V273T | Escherichia coli | |
0.18 | - |
fumarate | pH 7.7, 25°C, mutant V273A | Escherichia coli | |
0.21 | - |
Maleate | pH 7.7, 25°C, mutant V273N | Escherichia coli | |
0.64 | - |
fumarate | pH 7.7, 25°C, mutant V273T | Escherichia coli | |
0.89 | - |
succinate | pH 7.7, 25°C, wild-type | Escherichia coli | |
1 | - |
Maleate | pH 7.7, 25°C, mutant V273A | Escherichia coli | |
3.1 | - |
Maleate | pH 7.7, 25°C, wild-type | Escherichia coli |