Cloned (Comment) | Organism |
---|---|
purA gene | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
- |
Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D21A | directed mutagenesis | Escherichia coli |
N38A | directed mutagenesis | Escherichia coli |
N38D | directed mutagenesis | Escherichia coli |
N38E | directed mutagenesis | Escherichia coli |
R419L | directed mutagenesis | Escherichia coli |
T42A | directed mutagenesis | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
GTP | pH 5.6, 25°C, wild-type | Escherichia coli | |
0.026 | - |
GTP | pH 7.7, 25°C, wild-type | Escherichia coli | |
0.028 | - |
IMP | pH 7.7, 25°C, wild-type and mutant N38E | Escherichia coli | |
0.041 | - |
GTP | pH 7.7, 25°C, mutant N38A | Escherichia coli | |
0.048 | - |
IMP | pH 7.7, 25°C, mutant D21A | Escherichia coli | |
0.049 | - |
IMP | pH 7.7, 25°C, mutant N38A | Escherichia coli | |
0.052 | - |
GTP | pH 7.7, 25°C, mutant D21A | Escherichia coli | |
0.054 | - |
GTP | pH 7.7, 25°C, mutant N38E | Escherichia coli | |
0.07 | - |
IMP | pH 5.6, 25°C, wild-type | Escherichia coli | |
0.116 | - |
GTP | pH 5.6, 25°C, mutant N38D | Escherichia coli | |
0.123 | - |
IMP | pH 7.7, 25°C, mutant T42A | Escherichia coli | |
0.13 | - |
GTP | pH 7.7, 25°C, mutant H41N | Escherichia coli | |
0.17 | - |
aspartate | pH 7.7, 25°C, mutant D21A | Escherichia coli | |
0.2 | - |
IMP | pH 7.7, 25°C, mutant R419L | Escherichia coli | |
0.23 | - |
aspartate | pH 7.7, 25°C, wild-type | Escherichia coli | |
0.24 | - |
aspartate | pH 7.7, 25°C, mutant N38E | Escherichia coli | |
0.25 | - |
GTP | pH 7.7, 25°C, mutant R419L | Escherichia coli | |
0.27 | - |
GTP | pH 7.7, 25°C, mutant N38D | Escherichia coli | |
0.28 | - |
GTP | pH 7.7, 25°C, mutant T42A | Escherichia coli | |
0.3 | - |
aspartate | pH 7.7, 25°C, mutant N38A | Escherichia coli | |
0.34 | - |
aspartate | pH 7.7, 25°C, mutant R419L | Escherichia coli | |
0.4 | - |
IMP | pH 7.7, 25°C, mutant H41N | Escherichia coli | |
0.5 | - |
aspartate | pH 5.6, 25°C, mutant N38D | Escherichia coli | |
0.9 | - |
aspartate | pH 5.6, 25°C, wild-type | Escherichia coli | |
1.4 | - |
aspartate | pH 7.7, 25°C, mutant T42A | Escherichia coli | |
1.6 | - |
IMP | pH 7.7, 25°C, mutant N38D | Escherichia coli | |
2 | - |
aspartate | pH 7.7, 25°C, mutant H41N | Escherichia coli | |
2.6 | - |
aspartate | pH 7.7, 25°C, mutant N38D | Escherichia coli | |
3.8 | - |
IMP | pH 5.6, 25°C, mutant N38D | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + IMP + L-aspartate | Escherichia coli | catalyzing the first committed step in the biosynthesis of AMP | GDP + phosphate + adenylosuccinate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + IMP + L-aspartate | - |
Escherichia coli | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | catalyzing the first committed step in the biosynthesis of AMP | Escherichia coli | GDP + phosphate + adenylosuccinate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
adenosylsuccinate synthetase | - |
Escherichia coli |
AMPSase | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0048 | - |
GTP | pH 7.7, 25°C, mutant N38A | Escherichia coli | |
0.0095 | - |
GTP | pH 7.7, 25°C, mutant H41N | Escherichia coli | |
0.0134 | - |
GTP | pH 7.7, 25°C, mutant N38D | Escherichia coli | |
0.034 | - |
GTP | pH 7.7, 25°C, mutant N38E | Escherichia coli | |
0.049 | - |
GTP | pH 7.7, 25°C, mutant D21A | Escherichia coli | |
0.096 | - |
GTP | pH 5.6, 25°C, wild-type | Escherichia coli | |
0.1 | - |
GTP | pH 7.7, 25°C, mutant R419L | Escherichia coli | |
0.171 | - |
GTP | pH 5.6, 25°C, mutant N38D | Escherichia coli | |
0.89 | - |
GTP | pH 7.7, 25°C, mutant T42A | Escherichia coli | |
1 | - |
GTP | pH 7.7, 25°C, wild-type | Escherichia coli | |
1.59 | - |
GTP | pH 7.8, 25°C, wild-type | Escherichia coli | |
2.94 | - |
GTP | pH 7.8, 25°C, wild-type | Escherichia coli | |
6.08 | - |
GTP | pH 7.7, 25°C, mutant T42A | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.2 | 8 | - |
Escherichia coli |