BRENDA - Enzyme Database
show all sequences of 6.3.4.4

Human adenylosuccinate synthetase. Partial purification, kinetic and regulatory properties of the enzyme from placenta

Van der Weyden, M.B.; Kelly, W.N.; J. Biol. Chem. 249, 7282-7289 (1974)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
3',5'-cyclic GMP
-
Homo sapiens
6-mercaptopurine ribonucleotide
-
Homo sapiens
adenosine 3',5'-AMP
-
Homo sapiens
adenylosuccinate
competitive with respect to IMP
Homo sapiens
ADP
-
Homo sapiens
AMP
competitive with respect to IMP
Homo sapiens
CDP
-
Homo sapiens
CMP
-
Homo sapiens
dAMP
-
Homo sapiens
dCMP
-
Homo sapiens
dGDP
-
Homo sapiens
dGMP
-
Homo sapiens
GDP
competitive with respect to GTP
Homo sapiens
GMP
competitive with respect to GTP
Homo sapiens
IDP
-
Homo sapiens
succinate
-
Homo sapiens
TDP
-
Homo sapiens
TMP
-
Homo sapiens
UDP
-
Homo sapiens
UMP
-
Homo sapiens
XMP
competitive with respect to IMP
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.031
-
GTP
-
Homo sapiens
0.037
-
IMP
-
Homo sapiens
0.95
-
Asp
-
Homo sapiens
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ba2+
can replace Mg2+ with lower efficiency
Homo sapiens
Ca2+
can replace Mg2+ with lower efficiency
Homo sapiens
Co2+
can replace Mg2+ with lower efficiency
Homo sapiens
Mg2+
divalent cation required, maximal activity obtained with Mg2+
Homo sapiens
Mn2+
can replace Mg2+ with lower efficiency
Homo sapiens
Ni2+
can replace Mg2+ with lower efficiency
Homo sapiens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Purification (Commentary)
Commentary
Organism
partial
Homo sapiens
Reaction
Reaction
Commentary
Organism
GTP + IMP + L-aspartate = GDP + phosphate + N6-(1,2-dicarboxyethyl)-AMP
sequential rapid equilibrium fully random mechanism
Homo sapiens
Source Tissue
Source Tissue
Commentary
Organism
Textmining
placenta
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00641
-
-
Homo sapiens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
GTP + IMP + L-Asp
-
1548
Homo sapiens
GDP + phosphate + adenylosuccinate
-
1548
Homo sapiens
-
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
7.4
-
Homo sapiens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
3',5'-cyclic GMP
-
Homo sapiens
6-mercaptopurine ribonucleotide
-
Homo sapiens
adenosine 3',5'-AMP
-
Homo sapiens
adenylosuccinate
competitive with respect to IMP
Homo sapiens
ADP
-
Homo sapiens
AMP
competitive with respect to IMP
Homo sapiens
CDP
-
Homo sapiens
CMP
-
Homo sapiens
dAMP
-
Homo sapiens
dCMP
-
Homo sapiens
dGDP
-
Homo sapiens
dGMP
-
Homo sapiens
GDP
competitive with respect to GTP
Homo sapiens
GMP
competitive with respect to GTP
Homo sapiens
IDP
-
Homo sapiens
succinate
-
Homo sapiens
TDP
-
Homo sapiens
TMP
-
Homo sapiens
UDP
-
Homo sapiens
UMP
-
Homo sapiens
XMP
competitive with respect to IMP
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.031
-
GTP
-
Homo sapiens
0.037
-
IMP
-
Homo sapiens
0.95
-
Asp
-
Homo sapiens
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ba2+
can replace Mg2+ with lower efficiency
Homo sapiens
Ca2+
can replace Mg2+ with lower efficiency
Homo sapiens
Co2+
can replace Mg2+ with lower efficiency
Homo sapiens
Mg2+
divalent cation required, maximal activity obtained with Mg2+
Homo sapiens
Mn2+
can replace Mg2+ with lower efficiency
Homo sapiens
Ni2+
can replace Mg2+ with lower efficiency
Homo sapiens
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Homo sapiens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
placenta
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00641
-
-
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
GTP + IMP + L-Asp
-
1548
Homo sapiens
GDP + phosphate + adenylosuccinate
-
1548
Homo sapiens
-
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
7.4
-
Homo sapiens
Other publictions for EC 6.3.4.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744350
Karnawat
Exquisite modulation of the a ...
Methanocaldococcus jannaschii, Methanocaldococcus jannaschii DSM 2661
Biochemistry
55
2491-2499
2016
-
-
-
1
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4
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1
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-
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-
745423
Wang
Directed evolution of adenylo ...
Bacillus subtilis 168, Bacillus subtilis
J. Biotechnol.
231
115-121
2016
-
1
-
-
1
-
-
-
-
-
-
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3
-
-
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744526
Nomura
Biochemical analyses of ppGpp ...
Bacillus subtilis 168, Bacillus subtilis, Escherichia coli, Oryza sativa
Biosci. Biotechnol. Biochem.
78
1022-1025
2014
-
-
-
-
-
-
6
4
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-
93
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4
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4
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8
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6
8
4
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4
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4
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4
4
726626
Blundell
Purification, crystallization ...
Cryptococcus neoformans, Cryptococcus neoformans H99
Acta Crystallogr. Sect. F
69
1033-1036
2013
-
-
1
1
-
-
-
-
-
1
1
-
-
6
-
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1
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1
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1
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1
1
1
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1
1
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1
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727078
Mehrotra
Mutational analysis of cystein ...
Plasmodium falciparum
Biochim. Biophys. Acta
1824
589-597
2012
-
-
1
-
5
1
2
17
-
1
3
1
-
3
-
-
1
-
-
-
-
-
1
1
-
-
-
6
-
-
-
1
-
-
-
-
-
1
1
-
5
1
-
2
-
17
-
1
3
1
-
-
-
1
-
-
-
-
1
1
-
-
-
6
-
-
-
-
-
-
-
-
-
-
715099
Hitomi
Inosine triphosphate protects ...
Homo sapiens
Gastroenterology
140
1314-1321
2011
-
1
-
-
-
-
-
-
-
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3
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1
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1
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1
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1
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1
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1
1
-
-
-
721202
Wang
Overexpression, purification, ...
Pyrococcus horikoshii, Pyrococcus horikoshii OT-3
Acta Crystallogr. Sect. F
67
1551-1555
2011
-
-
1
1
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-
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1
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4
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1
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1
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1
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1
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1
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1
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-
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-
727072
Vemparala
Role of loop dynamics in therm ...
Escherichia coli, Pyrococcus horikoshii
Biochim. Biophys. Acta
1814
630-637
2011
-
-
-
-
-
-
-
-
-
-
-
2
-
4
-
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2
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2
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2
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2
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2
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714324
Mehrotra
Studies on active site mutants ...
Plasmodium falciparum
Biochim. Biophys. Acta
1804
1996-2002
2010
1
-
1
-
7
-
1
20
-
-
3
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4
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1
2
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8
-
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1
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1
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1
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7
-
-
1
1
20
-
-
3
-
-
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-
-
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-
-
1
2
-
-
-
8
-
-
-
-
-
-
-
-
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-
709915
Miller
Adenylosuccinate synthetase 1 ...
Mus musculus
Mol. Carcinog.
48
1116-1122
2009
-
-
-
-
-
-
-
-
-
-
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4
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1
1
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-
689055
Pimkin
The CBS subdomain of inosine 5 ...
Escherichia coli
Mol. Microbiol.
68
342-359
2008
-
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1
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1
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3
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2
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1
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690685
Zhang
An association study of ADSS g ...
Homo sapiens
Behav. Brain Funct.
4
39
2008
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1
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1
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1
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691098
Mehrotra
Methanocaldococcus jannaschii ...
Methanocaldococcus jannaschii
Biochim. Biophys. Acta
1784
2019-2028
2008
-
-
1
-
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1
4
-
1
3
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1
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1
2
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1
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1
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1
4
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1
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1
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1
2
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1
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692460
Oestreicher
The nadA gene of Aspergillus n ...
Aspergillus nidulans, Aspergillus nidulans pabaA1
Fungal Genet. Biol.
45
760-775
2008
2
-
-
-
-
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-
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3
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2
-
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695062
Honore
Identification of differential ...
Mus musculus
Proteome Sci.
6
18
2008
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1
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4
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1
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1
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673163
Li
-
Comparative molecular characte ...
Sus scrofa
Comp. Biochem. Physiol. B
147B
271-277
2007
-
-
1
-
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-
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2
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11
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1
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2
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13
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1
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726945
Mehrotra
Kinetic characterization of ad ...
Methanocaldococcus jannaschii, Methanocaldococcus jannaschii DSM 2661
Biochemistry
46
12821-12832
2007
-
-
1
-
-
-
8
3
-
1
1
-
-
6
-
-
1
1
-
-
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1
2
2
1
-
-
6
1
-
-
-
19
-
-
-
-
1
-
-
-
-
-
8
19
3
-
1
1
-
-
-
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1
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1
2
2
1
-
-
6
1
-
-
-
-
-
-
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-
672068
Iancu
Cavitation as a mechanism of s ...
Escherichia coli, Mus musculus
Biochemistry
45
11703-11711
2006
-
-
-
2
-
-
2
9
-
-
-
-
-
2
-
-
-
-
-
1
-
-
4
-
-
-
-
9
-
-
-
-
4
-
-
-
-
-
-
2
-
-
-
2
4
9
-
-
-
-
-
-
-
-
-
1
-
-
4
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
662843
Sun
Molecular cloning and characte ...
Homo sapiens
Mol. Cell. Biochem.
269
85-94
2005
-
-
1
-
-
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-
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1
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1
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4
-
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1
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6
-
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1
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-
-
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-
-
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-
-
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-
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-
1
-
-
-
-
-
-
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1
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1
-
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1
-
6
-
-
1
-
-
-
-
-
-
-
-
-
-
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-
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-
-
652929
Eaazhisai
Crystal structure of fully lig ...
Plasmodium falciparum
J. Mol. Biol.
335
1251-1264
2004
-
1
1
1
-
-
1
-
-
-
-
1
-
5
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
1
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-
1
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-
1
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-
-
1
-
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652965
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8
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2
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1
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3
1
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1550
Nagy
Regulation of the biosynthesis ...
Schizosaccharomyces pombe
Biochim. Biophys. Acta
309
1-10
1973
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