Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.3.4.4 extracted from

  • Bass, M.B.; Fromm, H.J.; Stayton, M.M.
    Overproduction, purification, and characterization of adenylosuccinate synthetase from Escherichia coli (1987), Arch. Biochem. Biophys., 256, 335-342.
    View publication on PubMed
Search for more literature for 6.3.4.4

Cloned(Commentary)

Cloned (Comment) Organism
cloned into the temperature-inducible, high-copy-number plasmid vector, pMOB45. Upon temperature induction, cells containing this plasmid produce adenylosuccinate synthetase at approximately 40times the wild-type level Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.038
-
 GTP
-
 Escherichia coli
0.054
-
 IMP
-
 Escherichia coli
0.98
-
 L-Asp
-
 Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 2 * 48000, SDS-PAGE Escherichia coli
141000
-
 HPLC gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + IMP + L-Asp Escherichia coli plays an important role in the interconversion of purines ?
-
 ?
GTP + IMP + L-Asp Escherichia coli first committed step towards the de novo biosynthesis of AMP ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 cloned into the temperature-inducible, high-copy-number plasmid vector, pMOB45. Upon temperature induction, cells containing this plasmid produce adenylosuccinate synthetase at approximately 40times the wild-type level
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.731
-
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + IMP + L-Asp
-
 Escherichia coli GDP + phosphate + adenylosuccinate
-
 ?
GTP + IMP + L-Asp plays an important role in the interconversion of purines Escherichia coli ?
-
 ?
GTP + IMP + L-Asp first committed step towards the de novo biosynthesis of AMP Escherichia coli ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 48000, SDS-PAGE Escherichia coli