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Literature summary for 6.3.4.3 extracted from
- Purification and properties of formyltetrahydrofolate synthetase (1980), Methods Enzymol., 66, 585-599.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Clostridium cylindrosporum |
General Stability
| General Stability | Organism |
|---|---|
| purified enzyme as crystalline suspension, 15% loss of activity after 1 month | Clostridium cylindrosporum |
| tetramer is stabilized by 0.1 M sulfate in absence of an active monovalent cation | Clostridium cylindrosporum |
| unstable in absence of monovalent cations | Clostridium cylindrosporum |
| unstable in presence of urea and guanidinium chloride | Clostridium cylindrosporum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,5'-dithiobis(2-nitrobenzoate) | - |
Clostridium cylindrosporum |  |
| Adenylyl imidodiphosphate | competitive to ATP | Clostridium cylindrosporum | |
| ADP | competitive to ATP | Clostridium cylindrosporum | |
| alpha,beta-methyleneadenosine 5'-triphosphate | competitive to ATP | Clostridium cylindrosporum | |
| beta,gamma-methyleneadenosine 5'-triphosphate | competitive to ATP | Clostridium cylindrosporum | |
| Formyltetrahydrofolate | - |
Clostridium cylindrosporum | |
| Mersalyl | - |
Clostridium cylindrosporum | |
| NEM | - |
Clostridium cylindrosporum | |
| p-hydroxymercuribenzoate | - |
Moorella thermoacetica | |
| phosphate | - |
Clostridium cylindrosporum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Oryctolagus cuniculus | |
| additional information | - |
additional information | - |
Moorella thermoacetica | |
| additional information | - |
additional information | monovalent cations decrease the Km for formate | Clostridium cylindrosporum | |
| 0.025 | - |
5,6,7,8-tetrahydropteroyltriglutamate | - |
Pisum sativum | |
| 0.025 | - |
5,6,7,8-tetrahydropteroyltriglutamate | - |
Clostridium cylindrosporum | |
| 0.12 | - |
ATP | - |
Saccharomyces cerevisiae | |
| 0.12 | - |
ATP | N10-formyltetrahydropteroyl-Glu3 | Clostridium cylindrosporum | |
| 0.13 | - |
ADP | formyltetrahydrofolate synthesis | Clostridium cylindrosporum | |
| 0.14 | - |
ATP | - |
Pisum sativum | |
| 0.14 | - |
ATP | - |
Clostridium cylindrosporum | |
| 0.22 | - |
ATP | - |
Clostridium cylindrosporum | |
| 5 | - |
phosphate | - |
Clostridium cylindrosporum | |
| 6.7 | - |
formate | wild-type enzyme | Clostridium cylindrosporum | |
| 10 | - |
N10-formyltetrahydrofolate | - |
Clostridium cylindrosporum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | the requirement for a divalent cation is most effectively satisfied by Mg2+. Mn2+ and Ca2+ can substitute for Mg2+ | Spinacia oleracea | |
| Ca2+ | the requirement for a divalent cation is most effectively satisfied by Mg2+. Mn2+ and Ca2+ can substitute for Mg2+ | Clostridium cylindrosporum | |
| Cs+ | - |
Spinacia oleracea | |
| Cs+ | specific monovalent cations required for maximal activity, order of effectiveness: NH4+, Tl+, Rb+ ~ K+, Cs+, Na+ ~ Li+ | Clostridium cylindrosporum | |
| divalent metal ion | the requirement for a divalent cation is most effectively satisfied by Mg2+. Mn2+ and Ca2+ can substitute for Mg2+ | Spinacia oleracea | |
| divalent metal ion | the requirement for a divalent cation is most effectively satisfied by Mg2+. Mn2+ and Ca2+ can substitute for Mg2+ | Clostridium cylindrosporum | |
| K+ | - |
Saccharomyces cerevisiae | |
| K+ | - |
Oryctolagus cuniculus | |
| K+ | - |
Spinacia oleracea | |
| K+ | - |
Moorella thermoacetica | |
| K+ | specific monovalent cations required for maximal activity, order of effectiveness: NH4+, Tl+, Rb+ ~ K+, Cs+, Na+ ~ Li+ | Clostridium cylindrosporum | |
| Li+ | specific monovalent cations required for maximal activity, order of effectiveness: NH4+, Tl+, Rb+ ~ K+, Cs+, Na+ ~ Li+ | Clostridium cylindrosporum | |
| Mg2+ | - |
Saccharomyces cerevisiae | |
| Mg2+ | - |
Pisum sativum | |
| Mg2+ | the requirement for a divalent cation is most effectively satisfied by Mg2+. Mn2+ and Ca2+ can substitute for Mg2+ | Spinacia oleracea | |
| Mg2+ | the requirement for a divalent cation is most effectively satisfied by Mg2+. Mn2+ and Ca2+ can substitute for Mg2+ | Clostridium cylindrosporum | |
| Mn2+ | the requirement for a divalent cation is most effectively satisfied by Mg2+. Mn2+ and Ca2+ can substitute for Mg2+ | Spinacia oleracea | |
| Mn2+ | the requirement for a divalent cation is most effectively satisfied by Mg2+. Mn2+ and Ca2+ can substitute for Mg2+ | Clostridium cylindrosporum | |
| monovalent cations | - |
Spinacia oleracea | |
| monovalent cations | specific monovalent cations required for maximal activity, order of effectiveness: NH4+, Tl+, Rb+ ~ K+, Cs+, Na+ ~ Li+ | Clostridium cylindrosporum | |
| Na+ | - |
Spinacia oleracea | |
| Na+ | specific monovalent cations required for maximal activity, order of effectiveness: NH4+, Tl+, Rb+ ~ K+, Cs+, Na+ ~ Li+ | Clostridium cylindrosporum | |
| NH4+ | - |
Oryctolagus cuniculus | |
| NH4+ | - |
Spinacia oleracea | |
| NH4+ | - |
Pisum sativum | |
| NH4+ | - |
Moorella thermoacetica | |
| NH4+ | specific monovalent cations required for maximal activity, order of effectiveness: NH4+, Tl+, Rb+ ~ K+, Cs+, Na+ ~ Li+ | Clostridium cylindrosporum | |
| Rb+ | - |
Spinacia oleracea | |
| Rb+ | specific monovalent cations required for maximal activity, order of effectiveness: NH4+, Tl+, Rb+ ~ K+, Cs+, Na+ ~ Li+ | Clostridium cylindrosporum | |
| Tl+ | specific monovalent cations required for maximal activity, order of effectiveness: NH4+, Tl+, Rb+ ~ K+, Cs+, Na+ ~ Li+ | Clostridium cylindrosporum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 240000 | - |
gel filtration, sedimentation analysis | Clostridium cylindrosporum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + formate + tetrahydrofolate | Gallus gallus | two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms | ? | - |
? | |
| ATP + formate + tetrahydrofolate | Escherichia coli | two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms | ? | - |
? | |
| ATP + formate + tetrahydrofolate | Homo sapiens | two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms | ? | - |
? | |
| ATP + formate + tetrahydrofolate | Saccharomyces cerevisiae | two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms | ? | - |
? | |
| ATP + formate + tetrahydrofolate | Oryctolagus cuniculus | two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms | ? | - |
? | |
| ATP + formate + tetrahydrofolate | Neurospora crassa | two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms | ? | - |
? | |
| ATP + formate + tetrahydrofolate | Ovis aries | two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms | ? | - |
? | |
| ATP + formate + tetrahydrofolate | Spinacia oleracea | two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms | ? | - |
? | |
| ATP + formate + tetrahydrofolate | Pisum sativum | two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms | ? | - |
? | |
| ATP + formate + tetrahydrofolate | Priestia megaterium | two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms | ? | - |
? | |
| ATP + formate + tetrahydrofolate | Pigeon | two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms | ? | - |
? | |
| ATP + formate + tetrahydrofolate | Proteus vulgaris | two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms | ? | - |
? | |
| ATP + formate + tetrahydrofolate | Clostridium cylindrosporum | two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms | ? | - |
? | |
| ATP + formate + tetrahydrofolate | Clostridium acidi-urici | two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms | ? | - |
? | |
| ATP + formate + tetrahydrofolate | Moorella thermoacetica | two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms | ? | - |
? | |
| ATP + formate + tetrahydrofolate | Micrococcus aerogenes | two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms | ? | - |
? | |
| ATP + formate + tetrahydrofolate | Veillonella parvula | two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium acidi-urici | - |
- |
- |
| Clostridium cylindrosporum | - |
- |
- |
| Escherichia coli | - |
- |
- |
| Gallus gallus | - |
- |
- |
| Homo sapiens | - |
trifunctional enzyme with 10-formyltetrahydrofolate synthetase, EC 6.3.4.3, 5,10-methenyltetrahydrofolate cyclohydrolase, EC 3.5.4.9, and 5,10-methylenetetrahydrofolate dehydrogenase activity, EC 1.5.1.5 | - |
| Micrococcus aerogenes | - |
- |
- |
| Moorella thermoacetica | - |
- |
- |
| Neurospora crassa | - |
- |
- |
| Oryctolagus cuniculus | - |
- |
- |
| Ovis aries | - |
trifunctional enzyme with 10-formyltetrahydrofolate synthetase, EC 6.3.4.3, 5,10-methenyltetrahydrofolate cyclohydrolase, EC 3.5.4.9, and 5,10-methylenetetrahydrofolate dehydrogenase activity, EC 1.5.1.5 | - |
| Pigeon | - |
- |
- |
| Pisum sativum | - |
- |
- |
| Priestia megaterium | - |
- |
- |
| Proteus vulgaris | - |
- |
- |
| Saccharomyces cerevisiae | - |
trifunctional enzyme with 10-formyltetrahydrofolate synthetase, EC 6.3.4.3, 5,10-methenyltetrahydrofolate cyclohydrolase, EC 3.5.4.9, and 5,10-methylenetetrahydrofolate dehydrogenase activity, EC 1.5.1.5 | - |
| Spinacia oleracea | - |
spinach | - |
| Veillonella parvula | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Clostridium cylindrosporum |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate | substrate binds to the enzyme in a random fashion, products are not released until all substrates are bound | Clostridium cylindrosporum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Spinacia oleracea | - |
| leaf | - |
Pisum sativum | - |
| leukocyte | - |
Homo sapiens | - |
| liver | - |
Gallus gallus | - |
| liver | - |
Oryctolagus cuniculus | - |
| liver | - |
Ovis aries | - |
| liver | - |
Pigeon | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Oryctolagus cuniculus |
| additional information | - |
- |
Pisum sativum |
| additional information | - |
- |
Clostridium cylindrosporum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + formate + 5,6,7,8-tetrahydropteroyltriglutamate | - |
Pisum sativum | ADP + phosphate + 10-formyltetrahydrofolyltriglutamate | - |
? | |
| ATP + formate + 5,6,7,8-tetrahydropteroyltriglutamate | - |
Clostridium cylindrosporum | ADP + phosphate + 10-formyltetrahydrofolyltriglutamate | - |
? | |
| ATP + formate + tetrahydrofolate | - |
Gallus gallus | ADP + phosphate + 10-formyltetrahydrofolate | - |
? | |
| ATP + formate + tetrahydrofolate | - |
Escherichia coli | ADP + phosphate + 10-formyltetrahydrofolate | - |
? | |
| ATP + formate + tetrahydrofolate | - |
Homo sapiens | ADP + phosphate + 10-formyltetrahydrofolate | - |
? | |
| ATP + formate + tetrahydrofolate | - |
Saccharomyces cerevisiae | ADP + phosphate + 10-formyltetrahydrofolate | - |
? | |
| ATP + formate + tetrahydrofolate | - |
Oryctolagus cuniculus | ADP + phosphate + 10-formyltetrahydrofolate | - |
? | |
| ATP + formate + tetrahydrofolate | - |
Neurospora crassa | ADP + phosphate + 10-formyltetrahydrofolate | - |
? | |
| ATP + formate + tetrahydrofolate | - |
Ovis aries | ADP + phosphate + 10-formyltetrahydrofolate | - |
? | |
| ATP + formate + tetrahydrofolate | - |
Spinacia oleracea | ADP + phosphate + 10-formyltetrahydrofolate | - |
? | |
| ATP + formate + tetrahydrofolate | - |
Pisum sativum | ADP + phosphate + 10-formyltetrahydrofolate | - |
? | |
| ATP + formate + tetrahydrofolate | - |
Priestia megaterium | ADP + phosphate + 10-formyltetrahydrofolate | - |
? | |
| ATP + formate + tetrahydrofolate | - |
Pigeon | ADP + phosphate + 10-formyltetrahydrofolate | - |
? | |
| ATP + formate + tetrahydrofolate | - |
Proteus vulgaris | ADP + phosphate + 10-formyltetrahydrofolate | - |
? | |
| ATP + formate + tetrahydrofolate | - |
Clostridium acidi-urici | ADP + phosphate + 10-formyltetrahydrofolate | - |
? | |
| ATP + formate + tetrahydrofolate | - |
Micrococcus aerogenes | ADP + phosphate + 10-formyltetrahydrofolate | - |
? | |
| ATP + formate + tetrahydrofolate | - |
Veillonella parvula | ADP + phosphate + 10-formyltetrahydrofolate | - |
? | |
| ATP + formate + tetrahydrofolate | r | Clostridium cylindrosporum | ADP + phosphate + 10-formyltetrahydrofolate | - |
? | |
| ATP + formate + tetrahydrofolate | r | Moorella thermoacetica | ADP + phosphate + 10-formyltetrahydrofolate | - |
? | |
| ATP + formate + tetrahydrofolate | ATP in form of MgATP2- | Saccharomyces cerevisiae | ADP + phosphate + 10-formyltetrahydrofolate | - |
? | |
| ATP + formate + tetrahydrofolate | ATP in form of MgATP2- | Pisum sativum | ADP + phosphate + 10-formyltetrahydrofolate | - |
? | |
| ATP + formate + tetrahydrofolate | ATP in form of MgATP2- | Clostridium cylindrosporum | ADP + phosphate + 10-formyltetrahydrofolate | - |
? | |
| ATP + formate + tetrahydrofolate | two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms | Gallus gallus | ? | - |
? | |
| ATP + formate + tetrahydrofolate | two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms | Escherichia coli | ? | - |
? | |
| ATP + formate + tetrahydrofolate | two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms | Homo sapiens | ? | - |
? | |
| ATP + formate + tetrahydrofolate | two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms | Saccharomyces cerevisiae | ? | - |
? | |
| ATP + formate + tetrahydrofolate | two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms | Oryctolagus cuniculus | ? | - |
? | |
| ATP + formate + tetrahydrofolate | two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms | Neurospora crassa | ? | - |
? | |
| ATP + formate + tetrahydrofolate | two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms | Ovis aries | ? | - |
? | |
| ATP + formate + tetrahydrofolate | two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms | Spinacia oleracea | ? | - |
? | |
| ATP + formate + tetrahydrofolate | two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms | Pisum sativum | ? | - |
? | |
| ATP + formate + tetrahydrofolate | two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms | Priestia megaterium | ? | - |
? | |
| ATP + formate + tetrahydrofolate | two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms | Pigeon | ? | - |
? | |
| ATP + formate + tetrahydrofolate | two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms | Proteus vulgaris | ? | - |
? | |
| ATP + formate + tetrahydrofolate | two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms | Clostridium cylindrosporum | ? | - |
? | |
| ATP + formate + tetrahydrofolate | two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms | Clostridium acidi-urici | ? | - |
? | |
| ATP + formate + tetrahydrofolate | two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms | Moorella thermoacetica | ? | - |
? | |
| ATP + formate + tetrahydrofolate | two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms | Micrococcus aerogenes | ? | - |
? | |
| ATP + formate + tetrahydrofolate | two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms | Veillonella parvula | ? | - |
? | |
| ATP + formate + tetrahydropteroyl-(Glu)n | - |
Oryctolagus cuniculus | ADP + phosphate + 10-formyltetrahydropteroyl-(Glu)n | - |
? | |
| ATP + formate + tetrahydropteroyl-(Glu)n | - |
Pisum sativum | ADP + phosphate + 10-formyltetrahydropteroyl-(Glu)n | - |
? | |
| ATP + formate + tetrahydropteroyl-(Glu)n | r, n: 3 | Clostridium cylindrosporum | ADP + phosphate + 10-formyltetrahydropteroyl-(Glu)n | - |
? | |
| Carbamoyl phosphate + ADP | - |
Clostridium cylindrosporum | ? + ATP | - |
? | |
| dATP + formate + tetrahydrofolate | 37% of the activity relative to ATP | Clostridium cylindrosporum | dADP + phosphate + 10-formyltetrahydrofolate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42 | - |
- |
Clostridium cylindrosporum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | - |
Clostridium cylindrosporum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9 | unstable below pH 6.5 and above pH 9.0 | Clostridium cylindrosporum |
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Release 2023.1 (January 2023)
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