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Literature summary for 6.3.4.3 extracted from
- Formyl-methenyl-methylenetetrahydrofolate synthetase (combined): a multifunctional protein in eukaryotic folate metabolism (1980), Methods Enzymol., 66, 616-626.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.056 | - |
ATP | - |
Sus scrofa |  |
| 0.086 | - |
ATP | - |
Ovis aries | |
| 0.12 | - |
ATP | - |
Saccharomyces cerevisiae | |
| 0.23 | - |
tetrahydrofolate | - |
Sus scrofa | |
| 0.46 | - |
tetrahydrofolate | - |
Ovis aries | |
| 2.3 | - |
tetrahydrofolate | - |
Saccharomyces cerevisiae | |
| 5.4 | - |
formate | - |
Ovis aries | |
| 16 | - |
formate | - |
Sus scrofa | |
| 38 | - |
formate | - |
Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 100000 | - |
x * 100000, SDS-PAGE | Sus scrofa |
| 100000 | - |
x * 100000, SDS-PAGE | Saccharomyces cerevisiae |
| 100000 | - |
x * 100000, SDS-PAGE | Ovis aries |
| 150000 | - |
gel filtration | Sus scrofa |
| 201000 | - |
gel filtration | Saccharomyces cerevisiae |
| 226000 | - |
gel filtration | Ovis aries |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + formate + tetrahydrofolate | Sus scrofa | the trifunctional enzyme is involved in the interconversion of one-carbon adducts of tetrahydrofolate | ? | - |
? | |
| ATP + formate + tetrahydrofolate | Saccharomyces cerevisiae | the trifunctional enzyme is involved in the interconversion of one-carbon adducts of tetrahydrofolate | ? | - |
? | |
| ATP + formate + tetrahydrofolate | Ovis aries | the trifunctional enzyme is involved in the interconversion of one-carbon adducts of tetrahydrofolate | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ovis aries | - |
trifunctional enzyme with 10-formyltetrahydrofolate synthetase, EC 6.3.4.3, 5,10-methenyltetrahydrofolate cyclohydrolase, EC 3.5.4.9, and 5,10-methylenetetrahydrofolate dehydrogenase activity, EC 1.5.1.5 | - |
| Saccharomyces cerevisiae | - |
trifunctional enzyme with 10-formyltetrahydrofolate synthetase, EC 6.3.4.3, 5,10-methenyltetrahydrofolate cyclohydrolase, EC 3.5.4.9, and 5,10-methylenetetrahydrofolate dehydrogenase activity, EC 1.5.1.5 | - |
| Sus scrofa | - |
trifunctional enzyme with 10-formyltetrahydrofolate synthetase, EC 6.3.4.3, 5,10-methenyltetrahydrofolate cyclohydrolase, EC 3.5.4.9, and 5,10-methylenetetrahydrofolate dehydrogenase activity, EC 1.5.1.5 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Ovis aries |
| large-scale purification | Saccharomyces cerevisiae |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Sus scrofa | - |
| liver | - |
Ovis aries | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Ovis aries |
| 51.3 | - |
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + formate + tetrahydrofolate | - |
Sus scrofa | ADP + phosphate + 10-formyltetrahydrofolate | - |
? | |
| ATP + formate + tetrahydrofolate | - |
Saccharomyces cerevisiae | ADP + phosphate + 10-formyltetrahydrofolate | - |
? | |
| ATP + formate + tetrahydrofolate | - |
Ovis aries | ADP + phosphate + 10-formyltetrahydrofolate | - |
? | |
| ATP + formate + tetrahydrofolate | ATP in form of MgATP2- | Sus scrofa | ADP + phosphate + 10-formyltetrahydrofolate | - |
? | |
| ATP + formate + tetrahydrofolate | ATP in form of MgATP2- | Saccharomyces cerevisiae | ADP + phosphate + 10-formyltetrahydrofolate | - |
? | |
| ATP + formate + tetrahydrofolate | ATP in form of MgATP2- | Ovis aries | ADP + phosphate + 10-formyltetrahydrofolate | - |
? | |
| ATP + formate + tetrahydrofolate | the trifunctional enzyme is involved in the interconversion of one-carbon adducts of tetrahydrofolate | Sus scrofa | ? | - |
? | |
| ATP + formate + tetrahydrofolate | the trifunctional enzyme is involved in the interconversion of one-carbon adducts of tetrahydrofolate | Saccharomyces cerevisiae | ? | - |
? | |
| ATP + formate + tetrahydrofolate | the trifunctional enzyme is involved in the interconversion of one-carbon adducts of tetrahydrofolate | Ovis aries | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 100000, SDS-PAGE | Sus scrofa |
| ? | x * 100000, SDS-PAGE | Saccharomyces cerevisiae |
| ? | x * 100000, SDS-PAGE | Ovis aries |
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